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Digestive Diseases and Sciences

Erschienen in:

01.10.2007 | Original Article

Gene Expression Profile Analysis of the Spontaneous Reversal of Rat Hepatic Fibrosis by cDNA Microarray

verfasst von: Qin Pan, Zhong-Bing Zhang, Xin Zhang, Jian Shi, Yue-Xiang Chen, Ze-Guang Han, Wei-Fen Xie

Erschienen in: Digestive Diseases and Sciences | Ausgabe 10/2007

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Abstract

Our aim was to gain insight into the gene expression profile during hepatic fibrosis autoreversal. Spontaneous recovery from hepatic fibrosis was created in SD rats by CCl₄ exposure for 8 weeks and then withdrawal for 6 weeks. Then differentially expressed genes during regression of fibrosis were analyzed using cDNA microarray. Results obtained were further subjected to hierarchical clustering and validated by semiquantitative RT-PCR. Expression of Mapk1 and Rps6ka1, which are critical members of the mitogen-activated protein kinase (MAPK) signaling pathway, was also investigated by Northern blot and immunohistochemistry. Microarray hybridization identified 254 genes differentially expressed throughout resolution of fibrosis. Being verified by RT-PCR, up- or down-regulated genes were classified into various groups according to clustering and function: (1) metabolic enzymes, (2) facilitated diffusion proteins/transporters/symporters, (3) gastrointestinal hormones/receptors, (4) lipoproteins/fatty acid binding proteins, (5) transcription factors/nuclear factors, and (6) the MAPK signaling pathway. The mRNA level of Mapk1 increased greatly as hepatic fibrosis reversed. Meanwhile Mapk1 and Rps6ka1 were proven to be expressed in hepatocytes and absent from mesenchymal cells. Six groups of genes exhibit a close relation to the recovery of CCl₄-induced hepatic fibrosis. The MAPK signaling-dependent pathway, representing one of the gene groups, may contribute to the reversal of hepatic fibrosis.

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Titel: Gene Expression Profile Analysis of the Spontaneous Reversal of Rat Hepatic Fibrosis by cDNA Microarray
verfasst von: Qin Pan
Zhong-Bing Zhang
Xin Zhang
Jian Shi
Yue-Xiang Chen
Ze-Guang Han
Wei-Fen Xie
Publikationsdatum: 01.10.2007
Verlag: Springer US
Erschienen in: Digestive Diseases and Sciences / Ausgabe 10/2007
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-006-9676-1

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