Skip to main content
Erschienen in: Archives of Virology 6/2013

01.06.2013 | Annotated Sequence Record

Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China

verfasst von: Jianfei Chen, Xiaozhen Liu, Hongwu Lang, Zhongtian Wang, Da Shi, Hongyan Shi, Xin Zhang, Li Feng

Erschienen in: Archives of Virology | Ausgabe 6/2013

Einloggen, um Zugang zu erhalten

Abstract

Porcine epidemic diarrhea virus (PEDV) poses a great threat to the Chinese swine industry. During 2006–2011, 74.0 % (94/127) of samples from 32 swine-raising farms in 15 provinces were positive for PEDV by reverse transcription nested polymerase chain reaction (RT-nested PCR). The sequences of nucleocapsid (N) genes of 32 representative field strains were determined, and the open reading frames (ORFs) of these N genes were 1326 nucleotides long. The N gene sequences were found to be more than 95 % identical to each other. The number of potential phosphorylation sites in the N protein varied from 5 to 12. A phylogenetic tree based on the N genes showed that the 32 Chinese field strains formed three groups.
Literatur
1.
Zurück zum Zitat Pensaert MB, de Bouck P (1978) A new coronavirus-like particles associated with diarrhea in swine. Arch Virol 58:243–247PubMedCrossRef Pensaert MB, de Bouck P (1978) A new coronavirus-like particles associated with diarrhea in swine. Arch Virol 58:243–247PubMedCrossRef
2.
Zurück zum Zitat Xuan H, Xing D, Wang D, Zhu W, Zhao F, Gong H (1984) Study on the culture of porcine epidemic diarrhea virus adapted to fetal porcine intestine primary cell monolayer. Chin J Vet Sci 4(3):202–208 Xuan H, Xing D, Wang D, Zhu W, Zhao F, Gong H (1984) Study on the culture of porcine epidemic diarrhea virus adapted to fetal porcine intestine primary cell monolayer. Chin J Vet Sci 4(3):202–208
3.
Zurück zum Zitat Tong Y, Feng L, Li W, Zhu Y, Wang M, Ma S (1999) Development of Bi-combined attenuated vaccine against transmissible gastroenteritis virus and porcine epidemic diarrhea virus. Chin J Pre Vet Med 21(6):406–410 Tong Y, Feng L, Li W, Zhu Y, Wang M, Ma S (1999) Development of Bi-combined attenuated vaccine against transmissible gastroenteritis virus and porcine epidemic diarrhea virus. Chin J Pre Vet Med 21(6):406–410
4.
Zurück zum Zitat Lecomte J, Cainelli-Gebara V, Mercier V, Mansour S, Talbot PJ, Lussier G, Oth D (1987) Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody. Arch Virol 97:123–130PubMedCrossRef Lecomte J, Cainelli-Gebara V, Mercier V, Mansour S, Talbot PJ, Lussier G, Oth D (1987) Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody. Arch Virol 97:123–130PubMedCrossRef
5.
Zurück zum Zitat Boots HAM, Benaissa-Trouw JB, Hesselink W, Rijke E, Schrier C, Hensen JE (1992) Induction of anti-viral immune responses by immunization with recombinant-DNA encoded avian coronavirus nucleocapsid protein. Vaccine 10:119–124PubMedCrossRef Boots HAM, Benaissa-Trouw JB, Hesselink W, Rijke E, Schrier C, Hensen JE (1992) Induction of anti-viral immune responses by immunization with recombinant-DNA encoded avian coronavirus nucleocapsid protein. Vaccine 10:119–124PubMedCrossRef
6.
Zurück zum Zitat Bridgen A, Duarte M, Tobler K, Laude H, Ackermann M (1993) Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus. J Gen Virol 74(9):1795–1804PubMedCrossRef Bridgen A, Duarte M, Tobler K, Laude H, Ackermann M (1993) Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus. J Gen Virol 74(9):1795–1804PubMedCrossRef
7.
Zurück zum Zitat Kubota S, Sasaki O, Amimoto K, Okada N, Kitazima T, Yasuhara H (1999) Detection of porcine epidemic diarrhea virus using polymerase chain reaction and comparison of the nucleocapsid protein genes among strains of the virus. J Vet Med Sci 61:827–830PubMedCrossRef Kubota S, Sasaki O, Amimoto K, Okada N, Kitazima T, Yasuhara H (1999) Detection of porcine epidemic diarrhea virus using polymerase chain reaction and comparison of the nucleocapsid protein genes among strains of the virus. J Vet Med Sci 61:827–830PubMedCrossRef
8.
Zurück zum Zitat Guscetti F, Bernasconi C, Tobler K, Van Reeth K, Pospischil A, Ackerman M (1998) Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods. Clin Diag Lab Immuno 5:412–414 Guscetti F, Bernasconi C, Tobler K, Van Reeth K, Pospischil A, Ackerman M (1998) Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods. Clin Diag Lab Immuno 5:412–414
9.
Zurück zum Zitat Duarte M, Tobler K, Bridgen A, Rasschaert D, Ackermann M, Laude H (1994) Sequence analysis of the porcine epidemic diarrhea virus genome between the nucleocapsid and spike protein genes reveals a polymorphic ORF. Virology 198:466–476PubMedCrossRef Duarte M, Tobler K, Bridgen A, Rasschaert D, Ackermann M, Laude H (1994) Sequence analysis of the porcine epidemic diarrhea virus genome between the nucleocapsid and spike protein genes reveals a polymorphic ORF. Virology 198:466–476PubMedCrossRef
10.
Zurück zum Zitat Chen H, Gill A, Dove BK, Emmett SR, Kemp CF, Ritchie MA, Dee M, Hiscox JA (2005) Mass spectroscopic characterization of the coronavirus infectious bronchitis virus nucleoprotein and elucidation of the role of phosphorylation in RNA binding by using surface plasmon resonance. J Virol 79:1164–1179PubMedCrossRef Chen H, Gill A, Dove BK, Emmett SR, Kemp CF, Ritchie MA, Dee M, Hiscox JA (2005) Mass spectroscopic characterization of the coronavirus infectious bronchitis virus nucleoprotein and elucidation of the role of phosphorylation in RNA binding by using surface plasmon resonance. J Virol 79:1164–1179PubMedCrossRef
11.
Zurück zum Zitat Park SJ, Moon HJ, Yang JS, Lee CS, Song DS, Kang BK, Park BK (2007) Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea. Virus Genes 35:321–332PubMedCrossRef Park SJ, Moon HJ, Yang JS, Lee CS, Song DS, Kang BK, Park BK (2007) Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea. Virus Genes 35:321–332PubMedCrossRef
12.
Zurück zum Zitat Lee D, Park CK, Kim SH, Lee C (2010) Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea. Virus Res 149:175–182PubMedCrossRef Lee D, Park CK, Kim SH, Lee C (2010) Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea. Virus Res 149:175–182PubMedCrossRef
13.
Zurück zum Zitat Park JY, Pak SI, Sung HW, Kim JH, Song CS, Lee CW, Kwon HM (2005) Variations in the nucleocapsid protein gene of infectious bronchitis viruses isolated in Korea. Virus Genes 31:153–162PubMedCrossRef Park JY, Pak SI, Sung HW, Kim JH, Song CS, Lee CW, Kwon HM (2005) Variations in the nucleocapsid protein gene of infectious bronchitis viruses isolated in Korea. Virus Genes 31:153–162PubMedCrossRef
14.
Zurück zum Zitat Kuo SM, Wang CH, Hou MH, Huang YP, Kao HW, Su HL (2010) Evolution of infectious bronchitis virus in Taiwan: characterisation of RNA recombination in the nucleocapsid gene. Vet Microbiol 144(3–4):293–302PubMedCrossRef Kuo SM, Wang CH, Hou MH, Huang YP, Kao HW, Su HL (2010) Evolution of infectious bronchitis virus in Taiwan: characterisation of RNA recombination in the nucleocapsid gene. Vet Microbiol 144(3–4):293–302PubMedCrossRef
15.
Zurück zum Zitat Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. MBE 28:2731–2739CrossRef Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. MBE 28:2731–2739CrossRef
Metadaten
Titel
Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China
verfasst von
Jianfei Chen
Xiaozhen Liu
Hongwu Lang
Zhongtian Wang
Da Shi
Hongyan Shi
Xin Zhang
Li Feng
Publikationsdatum
01.06.2013
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 6/2013
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-013-1608-8

Weitere Artikel der Ausgabe 6/2013

Archives of Virology 6/2013 Zur Ausgabe

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

„Jeder Fall von plötzlichem Tod muss obduziert werden!“

17.05.2024 Plötzlicher Herztod Nachrichten

Ein signifikanter Anteil der Fälle von plötzlichem Herztod ist genetisch bedingt. Um ihre Verwandten vor diesem Schicksal zu bewahren, sollten jüngere Personen, die plötzlich unerwartet versterben, ausnahmslos einer Autopsie unterzogen werden.

Hirnblutung unter DOAK und VKA ähnlich bedrohlich

17.05.2024 Direkte orale Antikoagulanzien Nachrichten

Kommt es zu einer nichttraumatischen Hirnblutung, spielt es keine große Rolle, ob die Betroffenen zuvor direkt wirksame orale Antikoagulanzien oder Marcumar bekommen haben: Die Prognose ist ähnlich schlecht.

Schlechtere Vorhofflimmern-Prognose bei kleinem linken Ventrikel

17.05.2024 Vorhofflimmern Nachrichten

Nicht nur ein vergrößerter, sondern auch ein kleiner linker Ventrikel ist bei Vorhofflimmern mit einer erhöhten Komplikationsrate assoziiert. Der Zusammenhang besteht nach Daten aus China unabhängig von anderen Risikofaktoren.

Semaglutid bei Herzinsuffizienz: Wie erklärt sich die Wirksamkeit?

17.05.2024 Herzinsuffizienz Nachrichten

Bei adipösen Patienten mit Herzinsuffizienz des HFpEF-Phänotyps ist Semaglutid von symptomatischem Nutzen. Resultiert dieser Benefit allein aus der Gewichtsreduktion oder auch aus spezifischen Effekten auf die Herzinsuffizienz-Pathogenese? Eine neue Analyse gibt Aufschluss.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.