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01.03.2014 | Original Contribution

Grape seed extract suppresses MDA-MB231 breast cancer cell migration and invasion

verfasst von: Simona Dinicola, Alessia Pasqualato, Alessandra Cucina, Pierpaolo Coluccia, Francesca Ferranti, Rita Canipari, Angela Catizone, Sara Proietti, Fabrizio D’Anselmi, Giulia Ricci, Alessandro Palombo, Mariano Bizzarri

Erschienen in: European Journal of Nutrition | Ausgabe 2/2014

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Abstract

Background and aim

Breast cancer remains a leading cause of mortality among women. In metastasis, cascade migration of cancer cells and invasion of extracellular matrix (ECM) represent critical steps. Urokinase-type plasminogen activator (uPA), as well as metalloproteinases MMP-2 and MMP-9, strongly contribute to ECM remodelling, thus becoming associated with tumour migration and invasion. In addition, the high expression of cytoskeletal (CSK) proteins, as fascin, has been correlated with clinically aggressive metastatic tumours, and CSK proteins are thought to affect the migration of cancer cells. Consumption of fruits and vegetables, characterized by high procyanidin content, has been associated to a reduced mortality for breast cancer. Therefore, we investigated the biological effect of grape seed extract (GSE) on the highly metastatic MDA-MB231 breast cancer cell line, focusing on studying GSE ability in inhibiting two main metastatic processes, i.e., cell migration and invasion.

Methods

After MDA-MB231 breast cancer cells stimulated with GSE migration and invasion were evaluated by means of trans-well assays and uPA as well as MMPs activity was detected by gelatin zymography. Fascin, β-catenin and nuclear factor-κB (NF-κB) expression were determined using western blot technique. β-Catenin localization was observed by confocal microscopy.

Results

We observed that high concentrations of GSE inhibited cell proliferation and apoptosis. Conversely, low GSE concentration decreased cell migration and invasion, likely by hampering β-catenin expression and localization, fascin and NF-κB expression, as well as by decreasing the activity of uPA, MMP-2 and MMP-9.

Conclusions

These results make GSE a powerful candidate for developing preventive agents against cancer metastasis.

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Titel: Grape seed extract suppresses MDA-MB231 breast cancer cell migration and invasion
verfasst von: Simona Dinicola
Alessia Pasqualato
Alessandra Cucina
Pierpaolo Coluccia
Francesca Ferranti
Rita Canipari
Angela Catizone
Sara Proietti
Fabrizio D’Anselmi
Giulia Ricci
Alessandro Palombo
Mariano Bizzarri
Publikationsdatum: 01.03.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 2/2014
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-013-0542-6

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Grape seed extract suppresses MDA-MB231 breast cancer cell migration and invasion

Abstract

Background and aim

Methods

Results

Conclusions

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