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Archives of Gynecology and Obstetrics

Erschienen in:

03.08.2016 | Gynecologic Endocrinology and Reproductive Medicine

Growth hormone co-treatment within a GnRH agonist long protocol improves implantation and pregnancy rates in patients undergoing IVF-ET

verfasst von: Xiao-fang Du, Xin-hong Yang, Jing Li, Mengmeng Hao, Yi-hong Guo

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 4/2016

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Abstract

Objective

The efficacy of growth hormone (GH) co-treatment within a GnRH agonist long regimen, in women with a normal ovarian response to controlled ovarian hyperstimulation (COH), for IVF was assessed.

Methods

This retrospective clinical trial was performed in a private-assisted reproduction centre. The study involved 1114 patients who responded normally to high-dose gonadotropin treatment. The study group of 556 patients was given in a daily subcutaneous injection of 4.5 IU of GH co-treatment, starting from the initial day of gonadotropin treatment and lasting for 5 days. The control group of 558 patients received the same treatment protocol without the GH co-treatment. The participants were further divided into two subgroups: age ≥35 years and age <35 years. The primary endpoint of the study was IVF-ET outcomes.

Results

The demographic characteristics did not significantly differ between the groups. The implantation rate (36.7 vs. 20.4 %, P < 0.05) and clinical pregnancy rate (57.3 vs. 30.1 %, P < 0.05) were significantly higher in the study group than in the control group. An analysis using a multivariate logistic regression model showed that GH was a significant factor for predicting pregnancy outcomes (OR 3.125, 95 % CI 2.441–4.000). Furthermore, for the ≥35-year-old group, the endometrial thickness was significantly greater (11.99 ± 2.21 vs. 11.62 ± 2.45, P < 0.05) in the study group than in the control group; in contrast, for the <35-year-old group, the high-quality embryo rate was significantly higher (71.7 vs. 68.3 %, P < 0.05) in the study group than in the control group.

Conclusion

Our study showed that co-treatment with GH in a GnRH agonist long protocol in patients who responded normally while undergoing IVF-ET could increase the implantation and pregnancy rates.

Nishiyama K, Sugimoto T, Kaji H, Kanatani M, Kobayashi T, Chihara K (1996) Stimulatory effect of growth hormone on bone resorption and osteoclast differentiation. Endocrinology 137:35–41. doi:10.1210/endo PubMed

Herrington J, Carter-Su C (2001) Signaling pathways activated by the growth hormone receptor. Trends Endocrinol Metab 12:252–257. doi:10.1016/S1043-2760(01)00423-4 CrossRefPubMed

Hull KL, Harvey S (2001) Growth hormone: roles in female reproduction. J Endocrinol 168:1–23. doi:10.1677/joe CrossRefPubMed

Homburg R, Eshel A, Abdalla HI, Jacobs HS (1988) Growth hormone facilitates ovulation induction by gonadotrophins. Clin Endocrinol (Oxf) 29:113–117. doi:10.1111/j CrossRef

Nakamura E, Otsuka F, Inagaki K, Miyoshi T, Matsumoto Y, Ogura K, Tsukamoto N, Takeda M, Makino H (2012) Mutual regulation of growth hormone and bone morpho genetic protein system in steroidogenesis by rat granulosa cells. Endocrinology 153:469–480. doi:10.1210/en CrossRefPubMed

Slot KA, Kastelijn J, Bachelot A, Kelly PA, Binart N, Teerds KJ (2006) Reduced recruitment and survival of primordial and growing follicles in GH receptor-deficient mice. Reproduction 131:525–532. doi:10.1530/rep CrossRefPubMed

Bachelot A, Monget P, Imbert-Bolloré P, Coshigano K, Kopchick JJ, Kelly PA, Binart N (2002) Growth hormone is required for ovarian follicular growth. Endocrinology 143:4104–4112. doi:10.1210/en CrossRefPubMed

Yoshimura Y, Nakamura Y, Koyama N, Iwashita M, Adachi T, Takeda Y (1993) Effects of growth hormone on follicle growth, oocyte maturation, and ovarian steroidogenesis. Fertil Steril 59:917–923CrossRefPubMed

Bevers MM, Izadyar F (2002) Role of growth hormone and growth hormone receptor in oocyte maturation. Mol Cell Endocrinol 197:173–178. doi:10.1016/S0303-7207(02)00270-8 CrossRefPubMed

10.

Yungai X, Li T (2006) The effect of growth hormone on endometrial receptivity in mice. J Med Forum 27:35–37

11.

Díaz-Gimeno P, Horcajadas JA, Martínez-Conejero JA, Esteban FJ, Alamá P, Pellicer A, Simón C (2011) A genomic diagnostic tool for human endometrial receptivity based on the transcriptomic signature. Fertil Steril 95:50–60. doi:10.1016/j CrossRefPubMed

12.

Achache H, Revel A (2006) Endometrial receptivity markers, the journey to successful embryo implantation. Hum Reprod Update 12:731–746. doi:10.1093/humupd/dml004 CrossRefPubMed

13.

Kolibianakis EM, Venetis CA, Diedrich K, Tarlatzis BC, Griesinger G (2009) Addition of growth hormone to gonadotrophins in ovarian stimulation of poor responders treated by in vitro fertilization: a systematic review and meta-analysis. Hum Reprod Update 15:613–622. doi:10.1093/humupd/dmp026 CrossRefPubMed

14.

Kyrou D, Kolibianakis EM, Venetis CA, Papanikolaou EG, Bontis J, Tarlatzis BC (2009) How to improve the probability of pregnancy in poor responders undergoing in vitro fertilization: a systematic review and meta-analysis. Fertil Steril 91:749–766. doi:10.1016/j CrossRefPubMed

15.

Wu MY, Chen HF, Ho HN, Chen SU, Chao KH, Huang SC, Lee TY, Yang YS (1996) The value of human growth hormone as an adjuvant for ovarian stimulation in a human in vitro fertilization program. J Obstet Gynaecol Res 22:443–450. doi:10.1111/j CrossRefPubMed

16.

Hughes SM, Huang ZH, Morris ID, Matson PL, Buck P, Lieberman BA (1994) A double-blind cross-over controlled study to evaluate the effect of human biosynthetic growth hormone on ovarian stimulation in previous poor responders to in vitro fertilization. Hum Reprod 9:13–18PubMed

17.

Boynukalın K, Güler T (2013) The use of growth hormone in in vitro fertilization. Basic Clin Sci 2:123–127CrossRef

18.

Var T, Tonguc E, Dogan M, Mollamahmutoglu L (2011) Relationship between the oestradiol/oocyte ratio and the outcome of assisted reproductive technology cycles with gonadotropin releasing hormone agonist. Gynecol Endocrinol 27:558–561. doi:10.3109/09513590 CrossRefPubMed

19.

Pereira GR, Lorenzo PL, Carneiro GF, Ball BA, Bilodeau-Goeseels S, Kastelic J, Pegoraro LM, Pimentel CA, Esteller-Vico A, Illera JC, Granado GS, Casey P, Liu IK (2013) The involvement of growth hormone in equine oocyte maturation, receptor localization and steroid production by cumulus-oocyte complexes in vitro. Res Vet Sci 95:667–674. doi:10.1016/j CrossRefPubMed

20.

Mendoza C, Ruiz-Requena E, Ortega E, Cremades N, Martinez F, Bernabeu R, Greco E, Tesarik J (2002) Follicular fluid markers of oocyte developmental potential. Hum Reprod 17:1017–1022. doi:10.1093/humrep/17 CrossRefPubMed

21.

Folch J, Ramón JP, Cocero MJ, Alabart JL, Beckers JF (2001) Exogenous growth hormone improves the number of transferable embryos in superovulated ewes. Theriogenology 55:1777–1785. doi:10.1016/S0093-691X(01)00520-9 CrossRefPubMed

22.

Moreira F, Badinga L, Burnley C, Thatcher WW (2002) Bovine somatotropin increases embryonic development in superovulated cows and improves post-transfer pregnancy rates when given to lactating recipient cows. Theriogenology 57:1371–1378. doi:10.1016/S0093-691X(01)00719-1 CrossRefPubMed

23.

Lanzone A, Fortini A, Fulghesu AM, Soranna L, Caruso A, Mancuso S (1996) Growth hormone enhances estradiol production follicle-stimulating hormone-induced in the early stage of the follicular maturation. Fertil Steril 66:948–953CrossRefPubMed

24.

Silva JRV, Figueiredo JR, van den Hurk R (2009) Involvement of growth hormone (GH) and insulin-likegrowth factor (IGF) system in ovarian folliculogenesis. Theriogenology 71:1193–1208. doi:10.1016/j CrossRefPubMed

25.

Kiapekou E, Loutradis D, Drakakis P, Zapanti E, Mastorakos G, Antsaklis A (2005) Effects of GH and IGF-I on the in vitro maturation of mouse oocytes. Hormones 4:155–160. doi:10.14310/horm CrossRefPubMed

26.

Yovich JL, Stanger JD (2010) Growth hormone supplementation improves implantation and pregnancy productivity rates for poor-prognosis patients undertaking IVF. Reprod Biomed Online 21:37–49. doi:10.1016/j CrossRefPubMed

27.

Kölle S, Stojkovic M, Reese S, Reichenbach HD, Wolf E, Sinowatz F (2004) Effects of growth hormone on the ultrastructure of bovine preimplantation embryos. Cell Tissue Res 317:101–108. doi:10.1007/s00441-004-0898-2 CrossRefPubMed

28.

Kidson A, Rubio-Pomar FJ, Van Knegsel A, Van Tol HT, Hazeleger W, Ducro-Steverink DW, Colenbrander B, Dieleman SJ, Bevers MM (2004) Quality of porcine blastocysts produced in vitro in the presence or absence of GH. Reproduction 127:165–177. doi:10.1530/rep CrossRefPubMed

29.

Xingxiang M, Yingchun Su, Yingpu Sun (2009) A meta analysis of effect of growth hormone on the development of endometrium. Prog Obstet Gynecol 18:330–332

30.

Sbracia M, Scarpellini F, Poverini R, Alò PL, Rossi G, Di Tondo U (2004) Immunohistochemical localization of the growth hormone in human endometrium and decidua. Am J Reprod Immunol 51:112–116. doi:10.1046/j CrossRefPubMed

31.

Zhang J, Tan L (2006) Effect of growth hormone on the thickness of proliferative endometrium and the expression of VEGF, EGF, IGF-I in mouse. J Med Forum 27:13–15

32.

Yang C, Fenghua L (2012) Effect of growth hormone on the endometrium receptivity in SD mice. J Biomed Eng 3:1674–1927

33.

Guijin Z, Bei X (2013) Progesterone regulate endometrial receptivity. J Reprod Med 22:272–275

34.

Spiliotis BE (2003) Growth hormone insufficiency and its impact on ovarian function. Ann N Y Acad Sci 997:77–84. doi:10.1196/annals CrossRefPubMed

Titel: Growth hormone co-treatment within a GnRH agonist long protocol improves implantation and pregnancy rates in patients undergoing IVF-ET
verfasst von: Xiao-fang Du
Xin-hong Yang
Jing Li
Mengmeng Hao
Yi-hong Guo
Publikationsdatum: 03.08.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 4/2016
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-016-4163-1

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Growth hormone co-treatment within a GnRH agonist long protocol improves implantation and pregnancy rates in patients undergoing IVF-ET