Background
Methods
Patients
Construction of the spiral array block and pathological Studies
Immunohistochemical staining
Cell culture
SERPINE2 knockdown by small interfering RNAs (siRNAs)
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR)
Effects of SERPINE2 in cell number
Cell migration assay and cell invasion assay
Fas ligand stimulation
Western blotting
Evidence from TCGA database
Statistical analysis
Results
Relationship between SERPINE2 expression and clinicopathologic factors
SERPINE2 | High | Low | P value |
---|---|---|---|
Number of case | 19 | 55 | |
Age [median (range)] | 69 (54–83) | 72 (49–85) | |
Male | 13 | 28 | 0.284 |
Smoking history | 14 | 30 | 0.181 |
Lobectomy | 16 | 40 | 0.372 |
Partial resection | 3 | 15 | |
pT1 | 10 | 26 | 0.847 |
pT2 | 9 | 28 | |
pT3 | 0 | 1 | |
pN0 | 16 | 43 | 0.121 |
pN1 | 0 | 8 | |
pN2 | 3 | 4 | |
pM0 | 19 | 55 | |
Pleural invasion | 8 | 19 | 0.589 |
Lymphatic invasion | 10 | 12 | 0.0188 |
Vascular invasion | 9 | 20 | 0.425 |
pStage IA | 11 | 25 | 0.163 |
pStage IB | 5 | 13 | |
pStage IIA | 0 | 11 | |
pStage IIB | 0 | 2 | |
pStage IIIA | 3 | 4 | |
R0 | 19 | 55 | |
EGFR mutation | |||
Ex21 L858R | 5 | 7 | 0.416 |
Ex19 del | 1 | 4 | |
Negative | 9 | 23 | |
Unknown | 12 | 13 | |
Adjuvant chemotherapy | 4 | 19 | 0.391 |
Relationship between SERPINE2 expression in immunostaining and overall survival
Variables | Cut off | Hazard ratio | 95% CI for HR | P value |
---|---|---|---|---|
pT factor | < 2 vs. ≥ 2 | 3.08 | 0.596–15.91 | 0.1795 |
pN factor | 0 vs. ≥ 1 | 2.787 | 0.6227–12.48 | 0.1801 |
Lymphatic invasion | 0 vs. 1 | 2.375 | 0.5291–10.66 | 0.93750 |
Pleural invasion | 0 vs. ≥ 1 | 3.383 | 0.7553–15.15 | 0.1111 |
Vascular invasion | 0 vs. 1 | 2.623 | 0.5863–11.73 | 0.2072 |
SERPINE2 | Low vs. high | 5.273 | 1.151–24.16 | 0.03228 |
Variables | Cut off | Hazard ratio | 95% CI for HR | P value |
---|---|---|---|---|
pT factor | < 2 vs. ≥ 2 | 2.2610 | 0.13140–38.900 | 0.57420 |
pN factor | 0 vs. ≥ 1 | 3.5320 | 0.34880–35.770 | 0.28540 |
Lymphatic invasion | 0 vs. 1 | 0.4981 | 0.06594–3.763 | 0.49940 |
Pleural invasion | 0 vs. ≥ 1 | 0.6023 | 0.04930–7.358 | 0.69130 |
Vascular invasion | 0 vs. 1 | 2.8550 | 0.31770–25.650 | 0.34910 |
SERPINE2 | Low vs. high | 9.0520 | 1.20300–68.090 | 0.03237 |