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Journal of Cancer Research and Clinical Oncology

Erschienen in:

01.04.2009 | Original Paper

HPV-16 E6 L83V variant in squamous cell carcinomas of the upper aerodigestive tract

verfasst von: Paolo Boscolo-Rizzo, Maria Cristina Da Mosto, Roberto Fuson, Helena Frayle-Salamanca, Rossana Trevisan, Annarosa Del Mistro

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 4/2009

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Abstract

Purpose

The aim of this prospective case series study was to determine the prevalence of HPV-DNA, analyze the E6 mRNA expression, identify intra-type variation in the E6 oncogene in upper aerodigestive tract (UADT) squamous cell carcinoma (SCC), and correlate the presence of HPV-DNA with several clinical parameters and outcome.

Methods

Frozen samples of UADT-SCC were analyzed for the presence and characterization of HPV-DNA and RNA sequences by means of polymerase chain reaction (PCR), reverse transcriptase-PCR, and direct sequencing of amplified products.

Results

HPV-DNA sequences were detected in 10% of the tumors, all of which were typed as HPV-16. Positivity for HPV-16 E6/E7 mRNA was observed in five of the eight HPV-positive tumors (62.5%). The HPV-16 E6 L83V variant was present in five cases. Multivariate analysis identified a history of absence of smoking (P = 0.009) as a predictor of HPV-positive tumor. No significant differences in overall and disease free survival curves were observed between patients with HPV-positive tumors and patients with tumors without detectable HPV-DNA.

Conclusion

Our findings support the etiological participation of HPV-16 in a subset of UADT-SCCs from patients lacking traditional risk factors. The potential prognostic significance of HPV-16 E6 L83V variant in HPV-16 positive UADT-SCCs should be more extensively investigated.

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Titel: HPV-16 E6 L83V variant in squamous cell carcinomas of the upper aerodigestive tract
verfasst von: Paolo Boscolo-Rizzo
Maria Cristina Da Mosto
Roberto Fuson
Helena Frayle-Salamanca
Rossana Trevisan
Annarosa Del Mistro
Publikationsdatum: 01.04.2009
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 4/2009
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-008-0490-3

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HPV-16 E6 L83V variant in squamous cell carcinomas of the upper aerodigestive tract