Skip to main content
Hauptrubriken
CME Facharzt-Training Webinare Zeitschriften Bücher e.Medpedia Podcast
Service
Jobbörse Info & Hilfe
Fachgebiete
Anästhesiologie Allgemeinmedizin Arbeitsmedizin Augenheilkunde Chirurgie Dermatologie Gynäkologie und Geburtshilfe HNO Innere Medizin Kardiologie Neurologie Onkologie und Hämatologie Orthopädie und Unfallchirurgie Pädiatrie Pathologie Psychiatrie Radiologie Rechtsmedizin Urologie Zahnmedizin
Themen
Klimawandel und Gesundheit Neues aus dem Markt COVID-19 Praxis und Beruf Seltene Erkrankungen GOÄ & EBM Panorama
Sammlungen
Kasuistiken Algorithmen & Infografiken Blickdiagnosen Arthropedia FlapFinder (für Dermatochirurgen) Videos Kongressberichterstattung Medizin für Apothekerinnen und Apotheker Für Ärztinnen und Ärzte in Weiterbildung Für Medizinstudierende
Erweiterte Suche
Anmelden
nach oben
Urolithiasis
Erschienen in: Urolithiasis 6/2016

09.06.2016 | Original Paper

Hyperoxaluria-induced tubular ischemia: the effects of verapamil on the antioxidant capacity of the affected kidneys

verfasst von: Kemal Sarica, Alper Kafkasli, Fehmi Narter, Oguz Ozturk, Ozgur Yazici, Bilal Hamarat, Cahit Sahin, Bilal Eryildirim

Erschienen in: Urolithiasis | Ausgabe 6/2016

Einloggen, um Zugang zu erhalten

Abstract

To evaluate the potential protective effects of a calcium channel blocker (Verapamil) on the oxidative stress related changes with an emphasis on the antioxidant capacity of the kidneys an experimental study in rats was performed. A total of 44 rats have been included. Hyperoxaluria was induced in Group 1 by continuous administration of ethylene glycol (EG). Animals in Group 2 received Verapamil in addition to EG. Animals in Group 3 constituted the control group. In addition to the evaluation of tissue and serum levels of three scavenging enzymes, NO, MDA and T-AOC; the presence and degree of crystal formation in renal parenchyma were evaluated in all animals after 7 and 28 days. Our data demonstrated that in addition to the lower level of all three scavenging enzymes (SOD, CAT and GSH) particularly during late phase evaluation (4 weeks); the total antioxidant capacity (T-AOC) of these kidneys were also higher when compared with the animals receiving EG only. Tissue and serum levels of both NO and MDA indicated the preventive effect of Verapamil on the oxidative stress induced changes. Very limited or no crystallization in the kidneys treated with verapamil during early and late phase examination was observed when compared with considerable crystal formation in Group 2 animals. Verapamil treatment may preserve the oxidant capacity of the kidneys and subsequently limit the crystal deposition induced by hyperoxaluria. Verapamil could therefore be considered in the management of kidney stone formation particularly in cases with recurrent kidney stone disease.
Literatur
1.
2.
Hackett RL, Shevock PN, Khan SR (1994) Madin-Darby canine kidney cells are injured by exposure to oxalate and to calcium oxalate crystals. Urol Res 22:197CrossRefPubMed
3.
Thamilselvan S, Khan SR (1998) Oxalate and calcium oxalate crystals are injurious to renal epithelial cells: results of in vivo and in vitro studies. J Nephrol 11:66PubMed
4.
Khan SR (1995) Calcium oxalate crystal interaction with renal tubular epithelium, mechanism of crystal adhesion and its impact on stone development. Urol Res 23:71CrossRefPubMed
5.
Khaskhali MH, Byer KJ, Khan SR (2009) The effect of calcium on calcium oxalate monohydrate crystal induced renal epithelial injury. Urol Res 37(1):1–6CrossRefPubMed
6.
Koul H, Kennington L, Honeyman T et al (1996) Activation of c-myc gene mediates the mitogenic effects of oxalate in LLC-PK1 cells, a line of renal epithelial cells. Kidney Int 50:1525–1530CrossRefPubMed
7.
Scheid C, Koul H, Hill WA et al (1996) Oxalate toxicity in LLC-PK1 cells, a line of renal epithelial cells. J Urol 155:1112–1116CrossRefPubMed
8.
Sarica K, Yagcı F, Bakır K, Erturhan S, Ucak R (2001) Renal tubular injury induced by hyperoxaluria: evaluation of apoptotic changes. Urol Res 29:34CrossRefPubMed
9.
Hackett RL, Shevock PN, Khan SR (1995) Alterations in MDCK and LLC-PK1 cells exposed to oxalate and calcium oxalate monohydrate crystals. Scanning Micorosc 9:587
10.
Davalos M, Konno S, Eshghi M, Choudhury M (2010) Oxidative renal cell injury induced by calcium oxalate crystal and reno protection with antioxidants: a possible role of oxidative stress in nephrolithiasis. J Endourol 24(3):339–345CrossRefPubMed
11.
Khan SR (2004) Crystal-induced inflammation of the kidneys: results from human studies, animal models and tissue-culture studies. Clin Exp Nephrol 8:75–88CrossRefPubMed
12.
Khan SR (2006) Renal tubular damage/dysfunction: key to the formation of kidney stones. Urol Res 34:86–91CrossRefPubMed
13.
Thamilselvan S, Khan SR, Menon M (2003) Oxalate and calcium oxalate mediated free radical toxicity in renal epithelial cells: effect of antioxidants. Urol Res 31:3–9PubMed
14.
Bhandari A, Koul S, Sekhon A et al (2002) Effects of oxalate on HK-2 cells, a line of proximal tubular epithelial cells from normal human kidney. J Urol 168:253–259CrossRefPubMed
15.
Khan SR (2014) Reactive oxygen species, inflammation and calcium oxalate nephrolithiasis. Transl Androl Urol 3(3):256–276PubMedPubMedCentral
16.
17.
Silva JP, Coutinho OP (2010) Free radicals in the regulation of damage and cell death – basicmechanisms and prevention. Drug Discov Ther 4(3):144–167PubMed
18.
Wilcox JK, Ash SL, Catignani GL (2004) Antioxidants and prevention of chronic disease. Crit Rev Food Sci Nutr 44:275–295CrossRef
19.
Halliwell B, Aeschbach R, Loliger J, Aruoma OI (1995) The characterization of antioxidants. Food Chem Toxicol 33:601–617CrossRefPubMed
20.
Biri H, Ozturk HS, Buyukkocak S, Kacmaz M (1998) Antioxidant defense potential of rabbit renal tissues after ESWL: protective effect of antioxidant vitamins. Nephron 79:181CrossRefPubMed
21.
Thamilselvan S, Byer KJ, Hackett RL, Khan SR (2000) Free radical scavengers, catalase and superoxide dismutase provide protection from oxalate associated injury to LLC-PK1 and MDCK cells. J Urol 164:224–229CrossRefPubMed
22.
Park HK, Jeong BC, Sung MK et al (2008) Reduction of oxidative stress in cultured renal tubular cells and preventive effects on renal stone formation by the bioflavonoid quercetin. J Urol 179(4):1620–1626CrossRefPubMed
23.
Khan SR, Shevock PN, Hackett RL (1993) Magnesium oxide administration and prevention of calcium oxalate nephrolithiasis. J Urol 149:412PubMed
24.
Sahin C, Sarikaya S, Sarica K et al (2015) Limitation of apoptotic changes and crystal deposition by Tutukon following hyperoxaluria-induced tubular cell injury in rat model. Urolithiasis 43(4):313–322CrossRefPubMed
25.
Tanriverdi O, Telci D, Aydin M, Ekici ID, Miroglu C, Sarıca K (2012) Hyperoxaluria-induced tubular ischemia: the effects of verapamil and vitamin E on apoptotic changes with an emphasis on renal papilla in rat model. Urol Res 40(1):17–25CrossRefPubMed
26.
Benyi L, Weilherg Z, Puyun L (1995) Protective effects of nifedipine and allopurinol on high energy shock wave induced acute changes of renal function. J Urol 153:596CrossRef
27.
Sarica K, Bakır K, Yagcı F, Topcu O, Akbay C, Sayın N, Korkmaz C (1999) Limitation of possible enhanced crystal deposition by verapamil in renal parenchyma after shock wave application in rabbit model. J Endourol 13:343CrossRefPubMed
28.
Strohmaier WL, Abelius A, Billes J, Grossmann T, Wilbert DM, Bichler KH (1994) Verapamil limits shock wave-induced renal tubular damage in vivo. J Endourol 8:269CrossRefPubMed
29.
Yencilek F, Sarica K, Eryildirim B, Erturhan S, Karakok M, Kuyumcuoglu U (2010) Hyperoxaluria-induced tubular ischemia: the effect of verapamil on the limitation of tissue HIF-1 alpha levels in renal parenchyma. Int Urol Nephrol 42(2):361–367CrossRefPubMed
30.
Sarica K, Erturhan S, Altay B (2007) Effect of verapamil on urinary stone-forming risk factors. Urol Res 35(1):23–27CrossRefPubMed
31.
Byer K, Khan SR (2005) Citrate provides protection against oxalate and calcium oxalate crystal induced oxidative damage to renal epithelium. J Urol 173(2):640–646CrossRefPubMed
32.
Tracy CR, Pearle MS (2009) Update on the medical management of stone disease. Curr Opin Urol 19(2):200–204CrossRefPubMed
33.
Huang HS, Chen J, Chen CF et al (2006) Vitamin E attenuates crystal formation in rat kidneys: roles of renal tubular cell death and crystallization inhibitors. Kidney Int 70:699–710CrossRefPubMed
34.
Huang HS, Chen J, Chen CF, Ma MC (2006) Vitamin E attenuates crystal formation in rat kidneys: roles of renal tubular cell death and crystallization inhibitors. Kidney Int 70(4):699–710 (erratum (2007) 71(7):712)
35.
Thamilselvan S, Hackett RL, Khan SR (1997) Lipid peroxidation in ethylene glycol induced hyperoxaluria and calcium Oxalate nephrolithiasis. J Urol 157:1059–1063CrossRefPubMed
36.
Muhtukumar A, Selvam R (1997) Renal injury mediated calcium oxalate nephrolithiasis: role of lipid peroxidation. Ren Fail 19:401–408CrossRef
37.
Huang HS, Ma MC, Chen CF et al (2003) Lipid peroxidation and its correlations with urinary levels of oxalate, citric acid, and osteopontin in patients with renal calcium oxalate stones. Urology 62:1123–1128CrossRefPubMed
38.
Strohmaier WL, Bichler KH, Koch J, Balk KN, Wilbert DM (1993) Protective effect of verapamil on shock wave-induced renal tubular dysfunction. J Urol 150:27PubMed
39.
Huang HS, Ma MC, Chen J et al (2002) Changes in the oxidant antioxidant balance in the kidney of rats with nephrolithiasis induced by ethylene glycol. J Urol 167:2584–2593CrossRefPubMed
40.
Dobashi K, Ghosh B, Orak JK, Singh I, Singh AK (2000) Kidney ischemia-reperfusion: modulation of antioxidant defenses. Mol Cell Biochem 205(1–2):1–11CrossRefPubMed
41.
Baggio B, Gambaro G, Ossi E et al (1983) Increased urinary excretion of renal enzymes in idiopathic calcium oxalate nephrolithiasis. J Urol 129:1161–1162PubMed
42.
Boonla C, Wunsuwan R, Tungsanga K et al (2007) Urinary 8-hydroxy-deoxyguanosine is elevated in patients with nephrolithiasis. Urol Res 35:185–191CrossRefPubMed
43.
Thamilselvan S, Byer KJ, Hackett RL et al (2000) Free radical scavengers, catalase and superoxide dismutase provide protection from oxalate-associated injury to LLC-PK1 and MDCK cells. J Urol 164:224–229CrossRefPubMed
44.
Gáspár S, Niculiţe C, Cucu D et al (2010) Effect of calcium oxalate on renal cells as revealed by real-time measurement of extracellular oxidative burst. Biosens Bioelectron 25:1729–1734CrossRefPubMed
45.
Messiha BA, Abo-Youssef AM (2015) Protective effects of fish oil, allopurinol, and verapamil on hepatic ischemia-reperfusion injury in rats. J Nat Sci Biol Med. Jul-Dec 6(2):351–355CrossRef
46.
Zhang J, Cao H, Zhang Y, Zhang Y et al (2013) Nephroprotective effect of calcium channel blockers against toxicity of lead exposure in mice. Toxicol Lett 218(3):273–280
47.
Bhattacharya SK, Rathi N, Mahajan P et al (2009) Effect of Ocimum sanctum, ascorbic acid, and verapamil on macrophage function and oxidative stress in mice exposed to cocaine. Indian J Pharmacol 41(3):134–9
48.
Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A (1993) A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84:407–412CrossRefPubMed
49.
Kusano C, Ferrari B (2008) Total antioxidant capacity: a biomarker in biomedical and nutritional studies. J. Cel. Mol. Biol. 7(1):1–15
50.
Iguchi M, Ikegami M, Kiwamoto H et al (1993) Effect of verapamil on urinary calcium and oxalate excretion in renal stone formers. Hinyokika Kiyo 39(5):425–431PubMed
51.
Jan CR, Chen WC, Wu SN et al (1998) Nifedipine, verapamil and diltiazem block shock-wave-induced rises in cytosolic calcium in MDCK cells. Chin J Physiol 41(4):181–188PubMed
52.
Tanaka T, Nangaku M, Miyata T et al (2004) Blockade of calcium influx through L-type calcium channels attenuates mitochondrial injury and apoptosis in hypoxic renal tubular cells. J Am Soc Nephrol 15(9):2320–2333CrossRefPubMed
53.
Ogawa Y, Hayashi K, Nakahama T et al (2001) Role of T-type calcium channels in renal microcirculation: studies in the isolated perfused hydronephrotic kidney. Hypertension 38:343–347CrossRef
54.
Epstein M (2002) Calcium antagonists in the mamagement of hypertension. Calcium antagonists in clinical medicine. Hanley and Belfus, Philadelphia, pp 293–313
55.
Goaling P (1986) Analytical reviews in clinical biochemistry: calcium measurement. Ann Clin Bichem 23:146CrossRef
56.
Krieg M, Gunsser KJ, Steinhagen-Tessen E, Becker H (1986) Comparative quantitative clinico-chemical analyses of the characteristics of 24-hour urine and morning urine. J Clin Chem Clin Biochem 24:863PubMed
57.
Frishman WH (1994) Current status of calcium channel blockers. Curr Probl Cardiol 19:637–688 (50)
58.
Carmines PK, Fowler BC, Bell PD (1993) Segmentally distinct effects of depolarization on intracellular [Ca2+] in renal arterioles. Am J Physiol 265(5 Pt 2):677–685
59.
Hayashi K, Nagahama T, Oka K, Epstein M, Saruta T (1996) Disparate effects of calcium antagonists on renal microcirculation. Hypertens Res 19(1):31–36CrossRefPubMed
60.
Grossman E, Messerli FH (1998) Effect of calcium antagonists on sympathetic activity. Eur Heart J 19(Suppl F):F27–F31PubMed
61.
Mason RP, Marche P, Hintze TH (2003) Novel vascular biology of third-generation L-type calcium channel antagonists: ancillary actions of amlodipine. Arterioscler Thromb Vasc Biol 23(12):2155–2163CrossRefPubMed
62.
Epstein M (1992) Calcium antagonists and renal protection: current status and future perspectives. Arch Intern Med 152:1573–1584CrossRefPubMed
63.
Epstein M (2002) Calcium antagonists and the kidney: implications for renal protection. Calcium antagonists in clinical medicine. Hanley and Belfus, Philadelphia, pp 629–664
64.
Epstein M (2002) Calcium antagonists in the management of hypertension. In: Calcium antagonists in clinical medicine. Hanley and Belfus, Philadelphia, pp 293–313
65.
Frishman WH (1994) Current status of calcium channel blockers. Curr Probl Cardiol 19:637–688CrossRefPubMed
66.
Danon A, Zenser TV, Thomasson DL et al (1990) Effect of verapamil on prostaglandin 2 synthesis by hydronephrotic rabbit cortical interstitial cells in primary culture. J Pharmacol Exp Ther 238:125–130
67.
Iguchi M, Ikegami M, Kiwamoto H et al (1993) Effect of verapamil on urinary calcium and oxalate excretion in renal stone formers. 39(5):425–431
Metadaten
Titel
Hyperoxaluria-induced tubular ischemia: the effects of verapamil on the antioxidant capacity of the affected kidneys
verfasst von
Kemal Sarica
Alper Kafkasli
Fehmi Narter
Oguz Ozturk
Ozgur Yazici
Bilal Hamarat
Cahit Sahin
Bilal Eryildirim
Publikationsdatum
09.06.2016
Verlag
Springer Berlin Heidelberg
Erschienen in
Urolithiasis / Ausgabe 6/2016
Print ISSN: 2194-7228
Elektronische ISSN: 2194-7236
DOI
https://doi.org/10.1007/s00240-016-0894-5

Weitere Artikel der Ausgabe 6/2016

Urolithiasis 6/2016 Zur Ausgabe

Review

Comparison of laparoscopic stone surgery and percutaneous nephrolithotomy in the management of large upper urinary stones: a meta-analysis

Original Paper

Stone volume is best predictor of operative time required in retrograde intrarenal surgery for renal calculi: implications for surgical planning and quality improvement

Original Paper

The influence of maternal and paternal history on stone composition and clinical course of calcium nephrolithiasis in subjects aged between 15 and 25

Original Paper

Effect of anxiety and pain on success of shockwave lithotripsy (SWL) for treatment of proximal ureteral and renal pelvic stones

Original Paper

The management of large staghorn renal stones by percutaneous versus laparoscopic versus open nephrolithotomy: a comparative analysis of clinical efficacy and functional outcome

Original Paper

Pulsed versus continuous mode fluoroscopy during PCNL: safety and effectiveness comparison in a case series study

Neu im Fachgebiet Urologie

Adjuvante Immuntherapie verlängert Leben bei RCC

25.04.2024 Nierenkarzinom Nachrichten

Nun gibt es auch Resultate zum Gesamtüberleben: Eine adjuvante Pembrolizumab-Therapie konnte in einer Phase-3-Studie das Leben von Menschen mit Nierenzellkarzinom deutlich verlängern. Die Sterberate war im Vergleich zu Placebo um 38% geringer.

Bei Senioren mit Prostatakarzinom auf Anämie achten!

24.04.2024 DGIM 2024 Nachrichten

Patienten, die zur Behandlung ihres Prostatakarzinoms eine Androgendeprivationstherapie erhalten, entwickeln nicht selten eine Anämie. Wer ältere Patienten internistisch mitbetreut, sollte auf diese Nebenwirkung achten.

Stufenschema weist Prostatakarzinom zuverlässig nach

22.04.2024 Prostatakarzinom Nachrichten

Erst PSA-Test, dann Kallikrein-Score, schließlich MRT und Biopsie – ein vierstufiges Screening-Schema kann die Zahl der unnötigen Prostatabiopsien erheblich reduzieren: Die Hälfte der Männer, die in einer finnischen Studie eine Biopsie benötigten, hatte einen hochgradigen Tumor.

Harnwegsinfektprophylaxe: Es geht auch ohne Antibiotika

20.04.2024 EAU 2024 Kongressbericht

Beim chronischen Harnwegsinfekt bei Frauen wird bisher meist eine Antibiotikaprophylaxe eingesetzt. Angesichts der zunehmenden Antibiotikaresistenz erweist sich das Antiseptikum Methenamin-Hippurat als vielversprechende Alternative, so die Auswertung einer randomisierten kontrollierten Studie.

Update Urologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen
Bildnachweise