Background
Methods
Parasites and culture
Sequence and bioinformatic analysis
Preparation of cDNA and real-time quantitative PCR
Genes ID | Forward primer | Reverse primer |
---|---|---|
PF3D7_1238600 | TTCCGGATGTATTTTGTTCC | CCCATTTGCTTAATTCATCG |
PF3D7_0107200 | CAGTTTATCCTTTTGAATATAATTAT | TGTTCAGGAGTATGTAAGATAAAAT |
PF3D7_1363500 | AGAAAACAACTTAATGCTATGTC | TGTCGAAAGTGGGTAAATTG |
PF3D7_0305600 | TAGACACTCAGGAATCGCAAGAAT | CCATAAGTTTGGTTTCTTTGTGAC |
PF3D7_0319200 | ACGGCTAGCCAAAGTAAC | GAATTCTGTTCCTCGTTTTT |
PF3D7_0519500 | AGTCCGGATATAGTATGTTTGC | CCCTCCTCTCCTTTTTCCT |
PF3D7_1430600 | AGCTGGAATGTAAATGGTTG | ACTGATTCGTTCGTTTTGGT |
Expression and purification of His-tagged and GST-tagged recombinant proteins
Genes ID | Primers | Recombinant plasmid | |
---|---|---|---|
PF3D7_1238600 | Forward primer | GGATCC TATGATATTGATATATTAGTTCT | pET-28a |
Reverse primer | CTCGAG ATTTGCAACTAGGATATAATCTA | ||
PF3D7_0107200 | Forward primer | GGATCC TTTAATAGCGCTTGGTATACA | pET-28a |
Reverse primer | CTCGAG ATGATCTGATGGGAAATGCTC | ||
PF3D7_1363500 | Forward primer | GGATCC ATACCTTCTGTGCGTAATATAAA | pET-28a |
Reverse primer | CTCGAG TAAGAGCTCAAATACTTTGG | ||
PF3D7_0305600 | Forward primer | GGATCC AATAATAATTCTCCCTTTAGTCA | pET-28a |
Reverse primer | CTCGAG TAAACTAAAATAAGCATTATAATC | ||
PF3D7_0319200 | Forward primer | GGATCC AAAAGTTATACATTTCCATATATA | pET-28a |
Reverse primer | TCTGAG TAAAAGCTTTAATTCCTTGTGAT |
Genes ID | Primers | Recombinant plasmid | |
---|---|---|---|
PF3D7_1238600 | Forward primer | GGATCC TATGATATTGATATATTAGTTCT | pGEX-4T-1 |
Reverse primer | CTCGAG ATTTGCAACTAGGATATAATCTA | ||
PF3D7_0107200 | Forward primer | GGATCC TTTAATAGCGCTTGGTATACA | pGEX-4T-1 |
Reverse primer | CTCGAG ATGATCTGATGGGAAATGCTC | ||
PF3D7_1363500 | Forward primer | GGATCC AAGGAATTTTCCGTTTTCTCTTT | pGEX-4T-1 |
Reverse primer | CTCGAG GTACGAATAAAATATATAATCTACAC | ||
PF3D7_0305600 | Forward primer | GGATCC ATTATTGTTACATGGAATATGAAT | pGEX-4T-1 |
Reverse primer | CTCGAG TTCATTTTTGAGGTATAATATAAC | ||
PF3D7_0319200 | Forward primer | GGATCC CGTATTCTATCGTATAATATTTTAGCA | pGEX-4T-1 |
Reverse primer | CTCGAG TAATTCTACCTCAGCAGCTATG |