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Journal of Cancer Research and Clinical Oncology

Erschienen in:

01.04.2008 | Original Paper

Identification and expression analysis of novel LAGE-1 alleles with single nucleotide polymorphisms in cancer patients

verfasst von: Yi Shao, Zhen-yuan Sun, Shi-wei Sun, Yi Zhao, Wan Yee Sin, Yan-hua Yuan, Andrew J. Simpson, Lloyd J. Old, Xin-ting Sang, Yi-lei Mao, Yong Xie, Jie-fu Huang, Hai-tao Zhao

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 4/2008

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Abstract

Objective

The purpose of this study is to identify single nucleotide polymorphisms (SNPs) in the coding region alleles of the X chromosomal LAGE-1 gene, and investigate the frequency of such SNPs in both cancer patients and healthy controls, and thus determine the potential significance of these SNPs with respect to cancer vaccine therapy.

Methods

In this study, different mRNAs transcribed from the LAGE-1 gene were identified by RT-PCR from healthy donors and cancer patients samples.

Results

A new LAGE-1 allele containing three coding region SNPs (69A/G, 317C/G, and 397T/G) were identified. The allele is highly expressed as the LAGE-1a mRNA variant AY679089 in some of the cancer patients. The three SNPs altered the LAGE-1 gene sequence to that of NYESO-1 at both the nucleotide and amino acid level.

Conclusion

There is a high frequency of the LAGE-1 gene allele with SNPs in coding regions in cancer patients. There was a clear relationship between the variant AY679089 and gastric cancer. The SNPs may lead to accelerated progress of poorly differentiated gastric cancer. The SNPs found in these alleles may also alter the immunological characteristics of LAGE-1a and should be taken into account if this antigen is adopted as a cancer vaccine component.

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Titel: Identification and expression analysis of novel LAGE-1 alleles with single nucleotide polymorphisms in cancer patients
verfasst von: Yi Shao
Zhen-yuan Sun
Shi-wei Sun
Yi Zhao
Wan Yee Sin
Yan-hua Yuan
Andrew J. Simpson
Lloyd J. Old
Xin-ting Sang
Yi-lei Mao
Yong Xie
Jie-fu Huang
Hai-tao Zhao
Publikationsdatum: 01.04.2008
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 4/2008
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-007-0312-z

Springer Medizin

Identification and expression analysis of novel LAGE-1 alleles with single nucleotide polymorphisms in cancer patients