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02.12.2019 | Original Article

Identification and imaging of indole-3-carboxamide cannabinoids in hair using matrix-assisted laser-desorption/ionization mass spectrometry

verfasst von: Houwei Lin, Xiaoyan Zeng, Qian Wang, Yinnan Li, Bin Sun, Ying Wang, Hang Wang

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Abstract

Purpose

Different kinds of new synthetic cannabinoids (SCs) have been continuously developed to evade drug monitoring. Segmental hair analysis offers a longer period for retrospective drug detection compared with blood or urine. In this study, matrix-assisted laser-desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometric imaging (MALDI-FT ICR MSI) was developed for direct identification and imaging of synthetic indole-3-carboxamide cannabinoids in hair samples using the positive ion mode.

Methods

The target SCs include N-(adamantan-1-yl)-1-pentyl-1H-indole-3-carboxamide (APICA), N-(1-amino-3-methyl-1-oxobutan-2-yl)-1-(5-fluoropentyl)-1H-indole-3-carboxamide (5F-AB-PICA), N-(1-amino-3,3-dimethyl-1-oxobutan-2-yl)-1-pentyl-1H-indazole-3-carboxamide (ADB-PINACA) and N-(1-amino -3,3-dimethyl-1-oxobutan-2-yl)-1-(5-fluoropentyl)-1H-indole-3-carboxamide (5F-ADBICA). The MALDI-MS and MS/MS were first performed on the scraped hair soaked in a mixture of the four SCs after matrix sublimation. This method may provide a detection power for SCs to the 0.1 ng level per 2 cm hair. Target cannabinoids were identified by MS¹ and MS². Matrix deposition methods including airbrush sprayer and sublimation were compared.

Results

The method was then applied in revealing the spatial distribution of APICA and 5F-ADBICA in real hair samples from two drug abusers by comparing MS¹ and MS² spectra. The metabolites of APICA and 5F-ADBICA were also presumed to be present in the positive hair samples. Furthermore, a comprehensive comparison between a MALDI-FT ICR MS and a MALDI time-of-flight–MS instrument was performed in detection-sensitivity and specificity for positive real samples.

Conclusions

The proposed method provides a powerful tool for drug supervision and forensic medicine analysis in a wide time window, and the sample amount required was also decreased.

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Titel: Identification and imaging of indole-3-carboxamide cannabinoids in hair using matrix-assisted laser-desorption/ionization mass spectrometry
verfasst von: Houwei Lin
Xiaoyan Zeng
Qian Wang
Yinnan Li
Bin Sun
Ying Wang
Hang Wang
Publikationsdatum: 02.12.2019
Verlag: Springer Singapore
Erschienen in: Forensic Toxicology
Print ISSN: 1860-8965
Elektronische ISSN: 1860-8973
DOI: https://doi.org/10.1007/s11419-019-00510-0

Springer Medizin

Identification and imaging of indole-3-carboxamide cannabinoids in hair using matrix-assisted laser-desorption/ionization mass spectrometry

Abstract

Purpose

Methods

Results

Conclusions

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

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Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie