Skip to main content
main-content

26.02.2019 | Original Article | Ausgabe 2/2019

Forensic Science, Medicine and Pathology 2/2019

Identification of a broad spectrum of mammalian and avian species using the short fragment of the mitochondrially encoded cytochrome b gene

Zeitschrift:
Forensic Science, Medicine and Pathology > Ausgabe 2/2019
Autoren:
Marko Andrejevic, Milica Keckarevic Markovic, Biljana Bursac, Milica Mihajlovic, Vanja Tanasic, Miljana Kecmanovic, Dusan Keckarevic
Wichtige Hinweise

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Abstract

Mitochondrial DNA (mtDNA), especially the gene for cytochrome b (MT-CYB), has been found to be highly informative for species identification. In this study, we present the results of the analysis of a 127 bp long fragment of MT-CYB, amplified using universal primers, variable enough to be used for species identification and discrimination, even in highly degraded animal samples. The total number of analyzed species in this study was 30, including 17 mammalian and 13 bird species. Using a newly created primer pair, we successfully amplified and sequenced the target sequence in almost all tested species. The amplification was incomplete in just two species, and as a result, partial, but still variable sequences, were obtained. Using the target fragment we successfully identified all tested samples. Initial results suggested that the intraspecies genetic diversity of the target region, in all tested species, was low – from 0 to 4.72%. The interspecies genetic diversity of the target region, crucial for successful discrimination, showed relatively high diversity, ranging from 8.36% to 42.52%. Given its short length, the target region should be used for species determination, particularly in samples that are degraded or are low in DNA quantity.

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

★ PREMIUM-INHALT
e.Med Interdisziplinär

Für Ihren Erfolg in Klinik und Praxis - Die beste Hilfe in Ihrem Arbeitsalltag als Mediziner

Mit e.Med Interdisziplinär erhalten Sie Zugang zu allen CME-Fortbildungen und Fachzeitschriften auf SpringerMedizin.de.

Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 2/2019

Forensic Science, Medicine and Pathology 2/2019 Zur Ausgabe

Neu im Fachgebiet Pathologie

26.01.2021 | Pneumonie | Schwerpunkt: Nicht-neoplastischen Lungenerkrankungen | Ausgabe 1/2021

Radiologische Besonderheiten interstitieller Lungenerkrankungen

21.01.2021 | Sarkoidose | Schwerpunkt: Nicht-neoplastischen Lungenerkrankungen | Ausgabe 1/2021

Übersicht: Granulomatöse Erkrankungen der Lunge

21.01.2021 | Tuberkulose | Schwerpunkt: Nicht-neoplastische Lungenerkrankungen | Ausgabe 1/2021

Molekularpathologie der Tuberkulose

Stellenwert, Methodik und Grenzen