nach oben

Pediatric Surgery International

Erschienen in:

01.02.2010 | Original Article

In vitro construction of scaffold-free cylindrical cartilage using cell sheet-based tissue engineering

verfasst von: Gakuto Tani, Noriaki Usui, Masafumi Kamiyama, Takaharu Oue, Masahiro Fukuzawa

Erschienen in: Pediatric Surgery International | Ausgabe 2/2010

Einloggen, um Zugang zu erhalten

Abstract

Purpose

Tissue-engineered cartilage may offer a solution for the treatment of serious airway disease. This study developed a novel procedure to fabricate a scaffold-free cylindrical cartilage under in vitro conditions, while also evaluating the effect of a dynamic culture on the engineered construct.

Methods

Auricular chondrocytes were harvested from New Zealand white rabbits and cultivated under high-density conditions to form a chondrocyte sheet. The sheet was looped around a silicon tube and cultivated for 6 weeks in dynamic or static conditions. The engineered cylindrical cartilages were evaluated macroscopically and histologically. The expression of collagen, glycosaminoglycan content and mechanical properties were determined.

Results

The cylindrical cartilage was sufficiently elastic and stiff to maintain the structure without disruption. Histologically, the construct contained a Safranin-O positive cartilaginous matrix accompanied by the expression of type II collagen. The glycosaminoglycan content increased and reached 72% of the native tracheal cartilage after 6 weeks of cultivation.

Conclusion

A novel procedure was developed for fabricating engineered cartilage, which maintained the shape and a proper level of rigidity and flexibility, under in vitro conditions using sheet-based tissue engineering techniques. This procedure may allow for the development of a tailor-made autograft and a functionally engineered trachea.

Watanabe T, Okuyama H, Kubota A, Kawahara H, Hasegawa T, Ueno T, Saka R, Morishita Y (2008) A case of tracheal agenesis surviving without mechanical ventilation after external esophageal stenting. J Pediatr Surg 43:1906–1908CrossRefPubMed

Martinod E, Seguin A, Pfeuty K, Fornes P, Kambouchner M, Azorin JF, Carpentier AF (2003) Long-term evaluation of the replacement of the trachea with an autologous aortic graft. Ann Thorac Surg 75:1572–1578CrossRefPubMed

Azorin JF, Bertin F, Martinod E, Laskar M (2006) Tracheal replacement with an aortic autograft. Eur J Cardiothorac Surg 29:261–263CrossRefPubMed

Tojo T, Niwaya K, Sawabata N, Kushibe K, Nezu K, Taniguchi S, Kitamura S (1998) Tracheal replacement with cryopreserved tracheal allograft: experiment in dogs. Ann Thorac Surg 66:209–213CrossRefPubMed

Vacanti CA, Paige KT, Kim WS, Sakata J, Upton J, Vacanti JP (1994) Experimental tracheal replacement using tissue-engineered cartilage. J Pediatr Surg 29:201–205CrossRefPubMed

Kojima K, Bonassar LJ, Roy AK, Vacanti CA, Cortiella J (2002) Autologous tissue-engineered trachea with sheep nasal chondrocytes. J Thorac Cardiovasc Surg 123:1177–1184CrossRefPubMed

Rotter N, Ung F, Roy AK, Vacanti M, Eavey RD, Vacanti CA, Bonassar LJ (2005) Role for interleukin 1alpha in the inhibition of chondrogenesis in autologous implants using polyglycolic acid-polylactic acid scaffolds. Tissue Eng 11:192–200CrossRefPubMed

Asplund B, Sperens J, Mathisen T, Hilborn J (2006) Effects of hydrolysis on a new biodegradable co-polymer. J Biomater Sci Polym Ed 17:615–630CrossRefPubMed

Grayson AC, Voskerician G, Lynn A, Anderson JM, Cima MJ, Langer R (2004) Differential degradation rates in vivo and in vitro of biocompatible poly (lactic acid) and poly (glycolic acid) homo- and co-polymers for a polymeric drug-delivery microchip. J Biomater Sci Polym Ed 15:1281–1304CrossRefPubMed

10.

Okano T, Yamada N, Sakai H, Sakurai Y (1993) A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropylacrylamide). J Biomed Mater Res 27:1243–1251CrossRefPubMed

11.

Nishida K, Yamato M, Hayashida Y, Watanabe K, Yamamoto K, Adachi E, Nagai S, Kikuchi A, Maeda N, Watanabe H, Okano T, Tano Y (2004) Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. N Engl J Med 351:1187–1196CrossRefPubMed

12.

Furukawa KS, Imura K, Tateishi T, Ushida T (2008) Scaffold-free cartilage by rotational culture for tissue engineering. J Biotechnol 133:134–145CrossRefPubMed

13.

Kaneshiro N, Sato M, Ishihara M, Mitani G, Sakai H, Kikuchi T, Mochida J (2007) Cultured articular chondrocytes sheets for partial thickness cartilage defects utilizing temperature-responsive culture dishes. Eur Cell Mater 13:87–92PubMed

14.

Naumann A, Dennis JE, Aigner J, Coticchia J, Arnold J, Berghaus A, Kastenbauer ER, Caplan AI (2004) Tissue engineering of autologous cartilage grafts in three-dimensional in vitro macroaggregate culture system. Tissue Eng 10:1695–1706CrossRefPubMed

15.

L’Heureux N, Dusserre N, Konig G, Victor B, Keire P, Wight TN, Chronos NA, Kyles AE, Gregory CR, Hoyt G, Robbins RC, McAllister TN (2006) Human tissue-engineered blood vessels for adult arterial revascularization. Nat Med 12:361–365CrossRefPubMed

16.

Park K, Huang J, Azar F, Jin RL, Min BH, Han DK, Hasty K (2006) Scaffold-free, engineered porcine cartilage construct for cartilage defect repair—in vitro and in vivo study. Artif Organs 30:586–596CrossRefPubMed

17.

Ando W, Tateishi K, Hart DA, Katakai D, Tanaka Y, Nakata K, Hashimoto J, Fujie H, Shino K, Yoshikawa H, Nakamura N (2007) Cartilage repair using an in vitro generated scaffold-free tissue-engineered construct derived from porcine synovial mesenchymal stem cells. Biomaterials 28:5462–5470CrossRefPubMed

18.

Wu W, Cheng X, Zhao Y, Chen F, Feng X, Mao T (2007) Tissue engineering of trachea-like cartilage grafts by using chondrocyte macroaggregate: experimental study in rabbits. Artif Organs 31:826–834CrossRefPubMed

19.

Weidenbecher M, Tucker HM, Awadallah A, Dennis JE (2008) Fabrication of a neotrachea using engineered cartilage. Laryngoscope 118:593–598CrossRefPubMed

20.

Nagai T, Furukawa KS, Sato M, Ushida T, Mochida J (2008) Characteristics of a scaffold-free articular chondrocyte plate grown in rotational culture. Tissue Eng Part A 14:1183–1193CrossRefPubMed

21.

Omori K, Nakamura T, Kanemaru S, Asato R, Yamashita M, Tanaka S, Magrufov A, Ito J, Shimizu Y (2005) Regenerative medicine of the trachea, the first human case. Ann Otol Rhinol Laryngol 114:429–433PubMed

22.

Yang J, Yamato M, Kohno C, Nishimoto A, Sekine H, Fukai F, Okano T (2005) Cell sheet engineering: recreating tissues without biodegradable scaffolds. Biomaterials 26:6415–6422CrossRefPubMed

23.

Watt FM (1988) Effect of seeding density on stability of the differentiated phenotype of pig articular chondrocytes in culture. J Cell Sci 89:373–378PubMed

24.

Schulze-Tanzil G, de Souza P, Villegas Castrejon H, John T, Merker HJ, Scheid A, Shakibaei M (2002) Redifferentiation of dedifferentiated human chondrocytes in high-density cultures. Cell Tissue Res 308:371–379CrossRefPubMed

25.

Kanzaki M, Yamato M, Hatakeyama H, Kohno C, Yang J, Umemoto T, Kikuchi A, Okano T, Onuki T (2006) Tissue engineering epithelial cell sheets for the creation of bioartificial trachea. Tissue Eng 12:1275–1283CrossRefPubMed

26.

Yang J, Yamato M, Shimizu T, Sekine H, Ohashi K, Kanzaki M, Ohki T, Nishida K, Okano T (2007) Reconstruction of functional tissue with cell sheet engineering. Biomaterials 28:5033–5043CrossRefPubMed

Titel: In vitro construction of scaffold-free cylindrical cartilage using cell sheet-based tissue engineering
verfasst von: Gakuto Tani
Noriaki Usui
Masafumi Kamiyama
Takaharu Oue
Masahiro Fukuzawa
Publikationsdatum: 01.02.2010
Verlag: Springer-Verlag
Erschienen in: Pediatric Surgery International / Ausgabe 2/2010
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-009-2543-3

Neu im Fachgebiet Pädiatrie

ADHS-Medikation erhöht das kardiovaskuläre Risiko

16.05.2024 Herzinsuffizienz Nachrichten

Erwachsene, die Medikamente gegen das Aufmerksamkeitsdefizit-Hyperaktivitätssyndrom einnehmen, laufen offenbar erhöhte Gefahr, an Herzschwäche zu erkranken oder einen Schlaganfall zu erleiden. Es scheint eine Dosis-Wirkungs-Beziehung zu bestehen.

Erstmanifestation eines Diabetes-Typ-1 bei Kindern: Ein Notfall!

16.05.2024 DDG-Jahrestagung 2024 Kongressbericht

Manifestiert sich ein Typ-1-Diabetes bei Kindern, ist das ein Notfall – ebenso wie eine diabetische Ketoazidose. Die Grundsäulen der Therapie bestehen aus Rehydratation, Insulin und Kaliumgabe. Insulin ist das Medikament der Wahl zur Behandlung der Ketoazidose.

Frühe Hypertonie erhöht späteres kardiovaskuläres Risiko

16.05.2024 Arterielle Hypertonie in der Pädiatrie Nachrichten

Wie wichtig es ist, pädiatrische Patienten auf Bluthochdruck zu screenen, zeigt eine kanadische Studie: Hypertone Druckwerte in Kindheit und Jugend steigern das Risiko für spätere kardiovaskuläre Komplikationen.

Betalaktam-Allergie: praxisnahes Vorgehen beim Delabeling

16.05.2024 Pädiatrische Allergologie Nachrichten

Die große Mehrheit der vermeintlichen Penicillinallergien sind keine. Da das „Etikett“ Betalaktam-Allergie oft schon in der Kindheit erworben wird, kann ein frühzeitiges Delabeling lebenslange Vorteile bringen. Ein Team von Pädiaterinnen und Pädiatern aus Kanada stellt vor, wie sie dabei vorgehen.

Update Pädiatrie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusion

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2010

Paediatric sutureless circumcision: a systematic literature review

The protective effect of erythropoietin on the acute phase of corrosive esophageal burns in a rat model

Submucosal fibrin glue injection for closure of recurrent tracheoesophageal fistula

Effect of leflunomide on liver regeneration after partial hepatectomy in rats

Long-term complications following operative intervention for intestinal malrotation: a 10-year review