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Clinical Neuroradiology
Erschienen in: Clinical Neuroradiology 1/2019

05.10.2017 | Original Article

In vivo Metabolic Profiles as Determined by 31P and short TE 1H MR-Spectroscopy

No Difference Between Patients with IDH Wildtype and IDH Mutant Gliomas

verfasst von: Katharina J. Wenger, Elke Hattingen, Kea Franz, Joachim Steinbach, Oliver Bähr, Ulrich Pilatus

Erschienen in: Clinical Neuroradiology | Ausgabe 1/2019

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Abstract

Purpose

Previous ex vivo spectroscopic data from tissue samples revealed differences in phospholipid metabolites between isocitrate dehydrogenase mutated (IDHmut) and IDH wildtype (IDHwt) gliomas. We investigated whether these changes can be found in vivo using 1H-decoupled 31P magnetic resonance spectroscopic imaging (MRSI) with 3D chemical shift imaging (CSI) at 3 T in patients with low and high-grade gliomas.

Methods

The study included 33 prospectively enrolled, mostly untreated patients who met spectral quality criteria according to the World Health Organization (WHO II n = 7, WHO III n = 17, WHO IV n = 9; 25 patients IDHmut, 8 patients IDHwt). The MRSI protocol included 1H decoupled 31P MRSI with 3D CSI (3D 31P CSI), 2D 1H CSI and a 1H single voxel spectroscopy sequence (TE 30 ms) from the tumor area. For 1H MRS, absolute metabolite concentration values were calculated (phantom replacement method). For 31P MRS, metabolite intensity ratios were calculated for the choline (C) and ethanolamine (E)-containing metabolites.

Results

In our patient cohort we did not find significant differences for the ratio of phosphocholine (PC) and phosphoethanolamine (PE), PC/PE, (p = 0.24) for IDHmut compared to IDHwt gliomas. Furthermore, we found no elevated ratios of glycerophosphocholine (GPC) and glycerophosphoethanolamine (GPE), GPC/GPE, (p = 0.68) or GPC/PE (p = 0.12) for IDHmut gliomas. Even the ratio (PC+GPC)/(PE+GPE) showed no significant differences with respect to mutation status (p = 0.16). Nonetheless, changes related to tumor grade regarding intracellular pH (pHi) and phospholipid metabolism as well as absolute metabolite concentrations of co-registered 2D 1H CSI data for tumor and control tissue showed the anticipated results.

Conclusion

Using 3D-CSI data acquisition, in vivo 31P MR spectroscopic measurement of phospholipid metabolites could not distinguish between IDHmut and IDHwt.
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Metadaten
Titel
In vivo Metabolic Profiles as Determined by 31P and short TE 1H MR-Spectroscopy
No Difference Between Patients with IDH Wildtype and IDH Mutant Gliomas
verfasst von
Katharina J. Wenger
Elke Hattingen
Kea Franz
Joachim Steinbach
Oliver Bähr
Ulrich Pilatus
Publikationsdatum
05.10.2017
Verlag
Springer Berlin Heidelberg
Erschienen in
Clinical Neuroradiology / Ausgabe 1/2019
Print ISSN: 1869-1439
Elektronische ISSN: 1869-1447
DOI
https://doi.org/10.1007/s00062-017-0630-8

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