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Erschienen in: Comparative Clinical Pathology 5/2017

27.05.2017 | Original Article

Inhibitory effects of lactic acid bacteria isolated from traditional fermented foods against aflatoxigenic Aspergillus spp.

verfasst von: Maryam Ebrahimi, Morteza Khomeiri, Ali Masoudi-Nejad, Alireza Sadeghi, Balal Sadeghi, Mahdi Kashaninejad

Erschienen in: Comparative Clinical Pathology | Ausgabe 5/2017

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Abstract

In the present study, after molecular identification of dominant lactic acid bacteria (LAB) isolated from traditional fermented foods, antifungal activity of the isolates was investigated against aflatoxigenic Aspergillus spp. Based on screening results, among the isolated LAB, Pediococus lolii had the highest inhibitory effect against Aspergillus flavus (70.32%) and Aspergillus niger (98.8%). Furthermore, antifungal activity of P. lolii stationary phase cell-free supernatant (CFS) was significantly (P < 0.05) higher than the effect of the logarithmic phase CFS. MIC values of P. lolii CFSs from logarithmic and stationary phases against A. flavus were 2 and 1%, and against A. niger were 4 and 1% (v/v), respectively. The media containing ≥4% of CFSs from logarithmic and stationary phases (v/v) had also totally inhibited from germination of A. flavus and A. niger spores. Safety assessment during 28-day oral administration of P. lolii revealed that there was no noticeable difference in specific growth rate, activity, behavior, hair luster, clinical chemistry, and hematological indices in treated rats in comparison to control group.
Literatur
Zurück zum Zitat Annan NT, Poll L, Sefa-Dedeh S, Plahar WA, Jakobsen M (2003) Volatile compounds produced by Lactobacillus fermentum, Saccharomyces cerevisiae and Candida krusei in single starter culture fermentations of Ghanaian maize dough. J Appl Microbiol 94:462–474CrossRefPubMed Annan NT, Poll L, Sefa-Dedeh S, Plahar WA, Jakobsen M (2003) Volatile compounds produced by Lactobacillus fermentum, Saccharomyces cerevisiae and Candida krusei in single starter culture fermentations of Ghanaian maize dough. J Appl Microbiol 94:462–474CrossRefPubMed
Zurück zum Zitat Avis TJ, Belanger RR (2001) Specificity and mode of action of the antifungal fatty acid cis-9-heptadecenoic acid produced by Pseudozyma flocculosa. Appl Environ Microb 67:956–960CrossRef Avis TJ, Belanger RR (2001) Specificity and mode of action of the antifungal fatty acid cis-9-heptadecenoic acid produced by Pseudozyma flocculosa. Appl Environ Microb 67:956–960CrossRef
Zurück zum Zitat Belguesmia Y, Choiset Y, Rabesona H, Baudy-Floc'h M, Le Blay G, Haertle T, Chobert JM (2013) Antifungal properties of durancins isolated from Enterococcus durans A5-11 and of its synthetic fragments. Lett Appl Microbiol 56:237–244CrossRefPubMed Belguesmia Y, Choiset Y, Rabesona H, Baudy-Floc'h M, Le Blay G, Haertle T, Chobert JM (2013) Antifungal properties of durancins isolated from Enterococcus durans A5-11 and of its synthetic fragments. Lett Appl Microbiol 56:237–244CrossRefPubMed
Zurück zum Zitat Bian X, Muhammad Z, Evivie SE, Luo WXM, Huo GC (2016) Screening of antifungal potentials of Lactobacillus helveticus KLDS 1.8701 against spoilage microorganism and their effects on physicochemical properties and shelf life of fermented soybean milk during preservation. Food Control 66:183–189CrossRef Bian X, Muhammad Z, Evivie SE, Luo WXM, Huo GC (2016) Screening of antifungal potentials of Lactobacillus helveticus KLDS 1.8701 against spoilage microorganism and their effects on physicochemical properties and shelf life of fermented soybean milk during preservation. Food Control 66:183–189CrossRef
Zurück zum Zitat Calvo AM, Gardner HW, Keller NP (2001) Genetic connection between fatty acid metabolism and sporulation in Aspergillus nidulans. J Biol Chem 276:25766–25774CrossRefPubMed Calvo AM, Gardner HW, Keller NP (2001) Genetic connection between fatty acid metabolism and sporulation in Aspergillus nidulans. J Biol Chem 276:25766–25774CrossRefPubMed
Zurück zum Zitat Dalie DKD, Deschamps AM, Atanasova-Penichon V, Richard-Forget F (2010) Potential of Pediococcus pentosaceus (L006) isolated from maize leaf to suppress fumonisin-producing fungal growth. J Food Protect 73:1129–1137CrossRef Dalie DKD, Deschamps AM, Atanasova-Penichon V, Richard-Forget F (2010) Potential of Pediococcus pentosaceus (L006) isolated from maize leaf to suppress fumonisin-producing fungal growth. J Food Protect 73:1129–1137CrossRef
Zurück zum Zitat Damodharan K, Lee YS, Palaniyandi SA, Yang SH, Suh JW (2015) Preliminary probiotic and technological characterization of Pediococcus pentosaceus strain KID7 and in vivo assessment of its cholesterol-lowering activity. Front Microbiol 6. doi:10.3389/fmicb.2015.00768 Damodharan K, Lee YS, Palaniyandi SA, Yang SH, Suh JW (2015) Preliminary probiotic and technological characterization of Pediococcus pentosaceus strain KID7 and in vivo assessment of its cholesterol-lowering activity. Front Microbiol 6. doi:10.​3389/​fmicb.​2015.​00768
Zurück zum Zitat Delavenne E, Cliquet S, Trunet C, Barbier G, Mounier J, Blay GL (2015) Characterization of the antifungal activity of Lactobacillus harbinensis K.V9.3.1Np and Lactobaciilus rhamnosus K.C8.3.11 in yogurt. Food Microbiol 45:10–17CrossRefPubMed Delavenne E, Cliquet S, Trunet C, Barbier G, Mounier J, Blay GL (2015) Characterization of the antifungal activity of Lactobacillus harbinensis K.V9.3.1Np and Lactobaciilus rhamnosus K.C8.3.11 in yogurt. Food Microbiol 45:10–17CrossRefPubMed
Zurück zum Zitat Digaitiene A, Hansen AS, Juodeikiene G, Eidukonyte D, Josephsen J (2012) Lactic acid bacteria isolated from rye sourdoughs produce bacteriocin-like inhibitory substances active against Bacillus subtilis and fungi. J Appl Microbiol 112:732–742CrossRefPubMed Digaitiene A, Hansen AS, Juodeikiene G, Eidukonyte D, Josephsen J (2012) Lactic acid bacteria isolated from rye sourdoughs produce bacteriocin-like inhibitory substances active against Bacillus subtilis and fungi. J Appl Microbiol 112:732–742CrossRefPubMed
Zurück zum Zitat Dong AR, Ho TT, Lo R, Bansal N, Turner MS (2017) A genetic diversity study of antifungal Lactobacillus plantarum isolates. Food Control 72:83–89CrossRef Dong AR, Ho TT, Lo R, Bansal N, Turner MS (2017) A genetic diversity study of antifungal Lactobacillus plantarum isolates. Food Control 72:83–89CrossRef
Zurück zum Zitat Gerbaldo GA, Barberis C, Pascual L, Dalcero A, Barberis L (2012) Antifungal activity of two Lactobacillus strains with potential probiotic properties. FEMS Microbiol Lett 332:27–33CrossRefPubMed Gerbaldo GA, Barberis C, Pascual L, Dalcero A, Barberis L (2012) Antifungal activity of two Lactobacillus strains with potential probiotic properties. FEMS Microbiol Lett 332:27–33CrossRefPubMed
Zurück zum Zitat Gulahmadov SG, Abdullaeva NF, Guseinova NF, Kuliev AA, Ivanova IV, Dalgalarondo M, Chobert JM, Haertlee T (2009) Isolation and characterization of bacteriocin-like inhibitory substances from lactic acid bacteria isolated from Azerbaijan cheeses. Appl Biochem Microbiol 45:266–271CrossRef Gulahmadov SG, Abdullaeva NF, Guseinova NF, Kuliev AA, Ivanova IV, Dalgalarondo M, Chobert JM, Haertlee T (2009) Isolation and characterization of bacteriocin-like inhibitory substances from lactic acid bacteria isolated from Azerbaijan cheeses. Appl Biochem Microbiol 45:266–271CrossRef
Zurück zum Zitat Jones ML, Martoni CJ, Tamber S, Parent M, Prakash S (2012) Evaluation of safety and tolerance of microencapsulated Lactobacillus reuteri NCIMB 30242 in a yogurt formulation: a randomized, placebo-controlled, double-blind study. Food Chem Toxicol 50:2216–2223CrossRefPubMed Jones ML, Martoni CJ, Tamber S, Parent M, Prakash S (2012) Evaluation of safety and tolerance of microencapsulated Lactobacillus reuteri NCIMB 30242 in a yogurt formulation: a randomized, placebo-controlled, double-blind study. Food Chem Toxicol 50:2216–2223CrossRefPubMed
Zurück zum Zitat Ju H, Youn H, Kwon J, Hong W, Song C (2016) Protective efficacy of Pediococcus lolii against Influenza a virus. Conference proceeding. Paper presented at International Scientific Conference on Probiotics and Prebiotics 2016, Budapest. In Kysucke Nove Mesto, by PAMIDA International. ISBN-978-80-89589-14-2 Ju H, Youn H, Kwon J, Hong W, Song C (2016) Protective efficacy of Pediococcus lolii against Influenza a virus. Conference proceeding. Paper presented at International Scientific Conference on Probiotics and Prebiotics 2016, Budapest. In Kysucke Nove Mesto, by PAMIDA International. ISBN-978-80-89589-14-2
Zurück zum Zitat Kabak B, Brandon EFA, Var I, Blokland M, Sips AJAM (2009) Effects of probiotic bacteria on the bioaccessibility of aflatoxin B1 and ochratoxin A using an in vitro digestion model under fed conditions. Journal of Environmental Science and Health 44:472–480CrossRefPubMed Kabak B, Brandon EFA, Var I, Blokland M, Sips AJAM (2009) Effects of probiotic bacteria on the bioaccessibility of aflatoxin B1 and ochratoxin A using an in vitro digestion model under fed conditions. Journal of Environmental Science and Health 44:472–480CrossRefPubMed
Zurück zum Zitat Kato N, Suyama S, Shirokane M, Kato M, Kobayashi T, Tsukagoshi T (2002) Novel α-glucosidase from Aspergillus nidulans with strong transglycosylation activity. Appl Environ Microb 68:1250–1256CrossRef Kato N, Suyama S, Shirokane M, Kato M, Kobayashi T, Tsukagoshi T (2002) Novel α-glucosidase from Aspergillus nidulans with strong transglycosylation activity. Appl Environ Microb 68:1250–1256CrossRef
Zurück zum Zitat Khanafari A, Soudi H, Miraboulfathi M (2007) Biocontrol of Aspergillus flavus and aflatoxin B1 production corn. Iran J Environ Health Sci Eng 4:163–168 Khanafari A, Soudi H, Miraboulfathi M (2007) Biocontrol of Aspergillus flavus and aflatoxin B1 production corn. Iran J Environ Health Sci Eng 4:163–168
Zurück zum Zitat Kohl J, Postma J, Nicot P, Ruocco M, Blum B (2011) Stepwise screening of microorganisms for commercial use in biological control of plant-pathogenic fungi and bacteria. Biol Control 57:1–12CrossRef Kohl J, Postma J, Nicot P, Ruocco M, Blum B (2011) Stepwise screening of microorganisms for commercial use in biological control of plant-pathogenic fungi and bacteria. Biol Control 57:1–12CrossRef
Zurück zum Zitat Lara-Villoslada F, Sierra S, Martín R, Delgado S, Rodríguez JM, Olivares M, Xaus J (2007) Safety assessment of two probiotic strains, Lactobacillus coryniformis CECT5711 and Lactobacillus gasseri CECT5714. J Appl Microbiol 103:175–184CrossRefPubMed Lara-Villoslada F, Sierra S, Martín R, Delgado S, Rodríguez JM, Olivares M, Xaus J (2007) Safety assessment of two probiotic strains, Lactobacillus coryniformis CECT5711 and Lactobacillus gasseri CECT5714. J Appl Microbiol 103:175–184CrossRefPubMed
Zurück zum Zitat Lavermicocca P, Valerio F, Evidente A, Lazzaroni S, Corsetti A, Gobbetti M (2000) Purification and characterization of novel antifungal compounds from the sourdough Lactobacillus plantarum strain 21B. Appl Environ Microb 66:4084–4090CrossRef Lavermicocca P, Valerio F, Evidente A, Lazzaroni S, Corsetti A, Gobbetti M (2000) Purification and characterization of novel antifungal compounds from the sourdough Lactobacillus plantarum strain 21B. Appl Environ Microb 66:4084–4090CrossRef
Zurück zum Zitat Lee JY, Nguyenb DT, Parka YS, Hwanga KY, Choa YS, Kanga KD, Yoonb J, Yuc J, Yeed SD, Ahne YH, Leeb G, Seonga SL, Paikd MJ (2012) Organic acid profiling analysis in culture media of lactic acid bacteria by gas chromatography-mass spectrometry. Mass Spectrom Lett 3:74–77CrossRef Lee JY, Nguyenb DT, Parka YS, Hwanga KY, Choa YS, Kanga KD, Yoonb J, Yuc J, Yeed SD, Ahne YH, Leeb G, Seonga SL, Paikd MJ (2012) Organic acid profiling analysis in culture media of lactic acid bacteria by gas chromatography-mass spectrometry. Mass Spectrom Lett 3:74–77CrossRef
Zurück zum Zitat Li H, Liu L, Zhang S, Cui W, Lv J (2012) Identification of antifungal compounds produced by Lactobacillus casei AST18. Curr Microbiol 65:156–161CrossRefPubMed Li H, Liu L, Zhang S, Cui W, Lv J (2012) Identification of antifungal compounds produced by Lactobacillus casei AST18. Curr Microbiol 65:156–161CrossRefPubMed
Zurück zum Zitat Magnusson J, Schnurer J (2001) Lactobacillus coryniformis subsp. coryniformis strain Si3 produces a broad-spectrum proteinaceous antifungal. Appl Environ Microb 67:1–5CrossRef Magnusson J, Schnurer J (2001) Lactobacillus coryniformis subsp. coryniformis strain Si3 produces a broad-spectrum proteinaceous antifungal. Appl Environ Microb 67:1–5CrossRef
Zurück zum Zitat Muhialdin BJ, Hassan Z, Bakar FA, Saari N (2016) Identification of antifungal peptides produced by Lactobacillus plantarum IS10 grown in the MRS broth. Food Control 59:27–30CrossRef Muhialdin BJ, Hassan Z, Bakar FA, Saari N (2016) Identification of antifungal peptides produced by Lactobacillus plantarum IS10 grown in the MRS broth. Food Control 59:27–30CrossRef
Zurück zum Zitat Özcelik S, Kuley E, Özogul F (2016) Formation of lactic, acetic, succinic, propionic, formic and butyric acid by lactic acid bacteria. LWT - Food Sci Technol 73:536–542CrossRef Özcelik S, Kuley E, Özogul F (2016) Formation of lactic, acetic, succinic, propionic, formic and butyric acid by lactic acid bacteria. LWT - Food Sci Technol 73:536–542CrossRef
Zurück zum Zitat Quiles JM, Saladino F, Manes J, Fernandez-Franzon M, Meca G (2016) Occurrence of mycotoxins in refrigerated pizza dough and risk assessment of exposure for the Spanish population. Food Chem Toxicol 94:19–24CrossRefPubMed Quiles JM, Saladino F, Manes J, Fernandez-Franzon M, Meca G (2016) Occurrence of mycotoxins in refrigerated pizza dough and risk assessment of exposure for the Spanish population. Food Chem Toxicol 94:19–24CrossRefPubMed
Zurück zum Zitat Rather IA, Seo BJ, Kumar VJR, Choi UH, Choi KH, Lim J, Park YH (2014) Biopreservative potential of Lactobacillus plantarum YML007 and efficacy as a replacement for chemical preservatives in animal feed. Food Sci Biotechnol 23:195–200CrossRef Rather IA, Seo BJ, Kumar VJR, Choi UH, Choi KH, Lim J, Park YH (2014) Biopreservative potential of Lactobacillus plantarum YML007 and efficacy as a replacement for chemical preservatives in animal feed. Food Sci Biotechnol 23:195–200CrossRef
Zurück zum Zitat Roy U, Batish VK, Grover S, Neelakantan S (1996) Production of antifungal substance by Lactococcus lactis subsp. lactis CHD-28.3. Int J Food Microbiol 32:27–34CrossRefPubMed Roy U, Batish VK, Grover S, Neelakantan S (1996) Production of antifungal substance by Lactococcus lactis subsp. lactis CHD-28.3. Int J Food Microbiol 32:27–34CrossRefPubMed
Zurück zum Zitat Russo P, Arena MP, Fiocco D, Capozzi V, Drider D, Spano G (2016) Lactobacillus plantarum with broad antifungal activity: a promising approach to increase safety and shelf-life of cereal-based products. Int J Food Microbiol In Press, Corrected Proof Russo P, Arena MP, Fiocco D, Capozzi V, Drider D, Spano G (2016) Lactobacillus plantarum with broad antifungal activity: a promising approach to increase safety and shelf-life of cereal-based products. Int J Food Microbiol In Press, Corrected Proof
Zurück zum Zitat Sadeghi A, Raeisi M, Ebrahimi M, Sadeghi B (2016) Antifungal activity of Pediococcus pentosaceus isolated from whole barley sourdough. J Food Qual Hazards Control 3:30–36 Sadeghi A, Raeisi M, Ebrahimi M, Sadeghi B (2016) Antifungal activity of Pediococcus pentosaceus isolated from whole barley sourdough. J Food Qual Hazards Control 3:30–36
Zurück zum Zitat Sangmanee P, Hongpattarakere T (2014) Inhibitory of multiple antifungal components produced by Lactobacillus plantarum K35 on growth, aflatoxin production and ultrastructure alterations of Aspergillus flavus and Aspergillus parasiticus. Food Control 40:224–233CrossRef Sangmanee P, Hongpattarakere T (2014) Inhibitory of multiple antifungal components produced by Lactobacillus plantarum K35 on growth, aflatoxin production and ultrastructure alterations of Aspergillus flavus and Aspergillus parasiticus. Food Control 40:224–233CrossRef
Zurück zum Zitat Sellamani M, Kalagatur NK, Siddaiah C, Mudili V, Krishna K, Natarajan G, Rao Putcha VL (2016) Antifungal and zearalenone inhibitory activity of Pediococcus pentosaceus isolated from dairy products on Fusarium graminearum. Front Microbiol 7: doi: 10.3389/fmicb.2016.00890 Sellamani M, Kalagatur NK, Siddaiah C, Mudili V, Krishna K, Natarajan G, Rao Putcha VL (2016) Antifungal and zearalenone inhibitory activity of Pediococcus pentosaceus isolated from dairy products on Fusarium graminearum. Front Microbiol 7: doi: 10.​3389/​fmicb.​2016.​00890
Zurück zum Zitat Sjogren J, Magnusson J, Broberg A, Schnürer J, Kenne L (2003) Antifungal 3-hydroxy fatty acids from Lactobacillus plantarum MiLAB 14. Appl Environ Microb 69:7554–7557CrossRef Sjogren J, Magnusson J, Broberg A, Schnürer J, Kenne L (2003) Antifungal 3-hydroxy fatty acids from Lactobacillus plantarum MiLAB 14. Appl Environ Microb 69:7554–7557CrossRef
Zurück zum Zitat Strom K, Sjogren J, Broberg A, Schnurer J (2002) Lactobacillus plantarum MiLAB 393 produces the antifungal cyclic dipeptides cyclo(L-Phe-L-Pro) and cyclo(L-Phe-trans-4-OH-L-Pro) and 3-phenyllactic acid. Appl Environ microb 68:4322–4327CrossRef Strom K, Sjogren J, Broberg A, Schnurer J (2002) Lactobacillus plantarum MiLAB 393 produces the antifungal cyclic dipeptides cyclo(L-Phe-L-Pro) and cyclo(L-Phe-trans-4-OH-L-Pro) and 3-phenyllactic acid. Appl Environ microb 68:4322–4327CrossRef
Zurück zum Zitat Sulemankhil I, Parent M, Jones ML, Feng Z, Labbe A, Prakash S (2012) In vitro and in vivo characterization and strain safety of Lactobacillus reuteri NCIMB 30253 for probiotic applications. Can J Microbiol 58:776–787CrossRefPubMed Sulemankhil I, Parent M, Jones ML, Feng Z, Labbe A, Prakash S (2012) In vitro and in vivo characterization and strain safety of Lactobacillus reuteri NCIMB 30253 for probiotic applications. Can J Microbiol 58:776–787CrossRefPubMed
Zurück zum Zitat Szabo NJ, DolanLC BGA, Shibano T, Sato S, Suzuki H, Uesugi T, Yamahira S, Toba M, Ueno H (2011) Safety evaluation of Lactobacillus pentosus strain b240. Food Chem Toxicol 49:251–258CrossRefPubMed Szabo NJ, DolanLC BGA, Shibano T, Sato S, Suzuki H, Uesugi T, Yamahira S, Toba M, Ueno H (2011) Safety evaluation of Lactobacillus pentosus strain b240. Food Chem Toxicol 49:251–258CrossRefPubMed
Zurück zum Zitat Tsai CC, Liu TH, Chen MH, Tsen HY (2004) Toxicity evaluation for an Enterococcus faecium strain TM39 in vitro and in vivo. Food Chem Toxicol 42:1601–1609CrossRefPubMed Tsai CC, Liu TH, Chen MH, Tsen HY (2004) Toxicity evaluation for an Enterococcus faecium strain TM39 in vitro and in vivo. Food Chem Toxicol 42:1601–1609CrossRefPubMed
Zurück zum Zitat Tsai CC, Leu SF, Huang QR, Chou LC, Huang CC (2014) Safety evaluation of multiple strains of Lactobacillus plantarum and Pediococcus pentosaceus in Wistar rats based on the Ames test and a 28-day feeding study. Scientific World J 2014: Article ID 928652, doi:10.1155/2014/928652 Tsai CC, Leu SF, Huang QR, Chou LC, Huang CC (2014) Safety evaluation of multiple strains of Lactobacillus plantarum and Pediococcus pentosaceus in Wistar rats based on the Ames test and a 28-day feeding study. Scientific World J 2014: Article ID 928652, doi:10.​1155/​2014/​928652
Zurück zum Zitat Varsha KK, Nishant G, Sneha SM, Shilpa G, Devendra L, Priya S, Nampoothiri KM (2016) Antifungal, anticancer and aminopeptidase inhibitory potential of a Phenazine compound produced by Lactococcus BSN307. Indian J Microbiol 56:411–416CrossRefPubMed Varsha KK, Nishant G, Sneha SM, Shilpa G, Devendra L, Priya S, Nampoothiri KM (2016) Antifungal, anticancer and aminopeptidase inhibitory potential of a Phenazine compound produced by Lactococcus BSN307. Indian J Microbiol 56:411–416CrossRefPubMed
Zurück zum Zitat Wang H, Yan Y, Wang J, Zhang H, Qi W (2012) Production and characterization of antifungal compounds produced by Lactobacillus plantarum IMAU10014. PLoS One 7:e29452CrossRefPubMedPubMedCentral Wang H, Yan Y, Wang J, Zhang H, Qi W (2012) Production and characterization of antifungal compounds produced by Lactobacillus plantarum IMAU10014. PLoS One 7:e29452CrossRefPubMedPubMedCentral
Zurück zum Zitat Yang EJ, Chang HC (2010) Purification of a new antifungal compound produced by Lactobacillus plantarum AF1 isolated from kimchi. Int J Food Microbiol 139:56–63CrossRefPubMed Yang EJ, Chang HC (2010) Purification of a new antifungal compound produced by Lactobacillus plantarum AF1 isolated from kimchi. Int J Food Microbiol 139:56–63CrossRefPubMed
Zurück zum Zitat Yang E, Fan L, Yueming J, Doucette C, Fillmore S (2012) Antimicrobial activity of bacteriocin-producing lactic acid bacteria isolated from cheeses and yogurts. AMB Express 2:48–60CrossRefPubMedPubMedCentral Yang E, Fan L, Yueming J, Doucette C, Fillmore S (2012) Antimicrobial activity of bacteriocin-producing lactic acid bacteria isolated from cheeses and yogurts. AMB Express 2:48–60CrossRefPubMedPubMedCentral
Metadaten
Titel
Inhibitory effects of lactic acid bacteria isolated from traditional fermented foods against aflatoxigenic Aspergillus spp.
verfasst von
Maryam Ebrahimi
Morteza Khomeiri
Ali Masoudi-Nejad
Alireza Sadeghi
Balal Sadeghi
Mahdi Kashaninejad
Publikationsdatum
27.05.2017
Verlag
Springer London
Erschienen in
Comparative Clinical Pathology / Ausgabe 5/2017
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-017-2489-0

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