4.1 Intranasal Insulin Effects in Humans with Mild Cognitive Impairment and AD
Interest in the role of brain insulin signaling in the development of AD and in methods to improve insulin action in the CNS to prevent disease progression has intensified in recent years [e.g.,
129‐
131]. This interest has been stoked by pioneering studies conducted by Suzanne Craft and colleagues indicating that the beneficial effects of IN insulin on declarative memory outlined above are not restricted to healthy participants but can also be found in people with mild cognitive impairment (MCI) or (early) AD (see [
132] for a systematic review covering relevant research up to October 2017).
In a study in 23 men and women with AD and 14 aged-matched healthy controls who were all non-diabetic, intravenous insulin in comparison with placebo improved story recall, a measure of declarative memory function, and selective attention assessed with the Stroop interference test [
133]. Subsequent trials made use of the IN paradigm. In a comparison of 13 adult men and women with early AD and 13 men and women with MCI, matched with 35 controls, the acute effect of IN insulin was investigated in three conditions (placebo [saline], 20 IU and 40 IU of insulin administered 15 min before cognitive assessments) [
134]. The cognitive test battery assessed verbal declarative memory (story recall and word-list recall), visual working memory (self-ordered pointing task), selective attention (Stroop test), and visual search. Intranasal insulin compared with placebo improved both measures of recall only in memory-impaired apoE ε4-negative participants, whereas healthy controls did not benefit and memory-impaired apoE ε4 carriers even showed signs of insulin-induced deterioration of word-list recall. Follow-up studies found comparable patterns: apoE ε4-negative participants with memory impairments benefited from acute IN insulin vs placebo (saline) delivery in terms of memory improvement whereas apoE ε4 carriers demonstrated a relative decline [
135]. Adults with MCI including amnestic symptoms (e.g., due to AD) who were treated with IN insulin for 3 weeks (2 × 20 IU/day,
n = 13) showed significantly increased story recall compared with participants treated with a placebo (saline;
n = 12) [
136]. The observation of apoE ε4-dependent differences in the impact of IN insulin raises the possibility that brain insulin signaling may only be impaired, and therefore a particularly worthwhile target of interventions, in patients without the apoE ε4 allele [
137], which has received further support in subsequent trials [
41,
70] (for conflicting data see e.g.,
138).
In a pilot clinical trial lasting 4 months [
139], women and men diagnosed with MCI or mild-to-moderate AD received 40 IU of regular insulin, placebo (saline), or 40 IU of insulin detemir (each
n = 12), a long-acting insulin analog with relatively high lipophilicity that has been assumed to exert stronger effects on brain functions than regular insulin [
138,
140]. Cognitive tests included delayed story recall, the Alzheimer Disease Assessment Scale-cognitive subscale 12 (ADAS-Cog-12 [
141]), and the Dementia Severity Rating Scale [
142]. Intranasal delivery of regular insulin compared with placebo improved memory scores after 2 and 4 months of treatment and was associated with preserved magnetic resonance imaging (MRI)-assessed brain volumes in the left superior parietal cortex, right middle cingulum, left cuneus, and right parahippocampal gyrus. Surprisingly, insulin detemir administration remained without effects. In a related 4-month trial [
143], male and female adults with amnestic MCI or mild-to-moderate AD received placebo (saline;
n = 30) or 20 IU (
n = 36) or 40 IU (
n = 38) of regular insulin/day. In comparisons with the placebo group, story recall after a delay of 20 min was enhanced in the 20-IU but not in the 40-IU group, while caregiver-rated functional ability was preserved in both insulin-treated groups; moreover, the progression of hypometabolism assessed via FDG-PET was dampened in both insulin groups. Findings like these suggest that there may be an optimal regimen of IN insulin administration between doses that are too low and, notably, too high, i.e., a inverted U-shaped function of beneficial insulin effects. This assumption has received support in acute experiments by Suzanne Craft’s group [
135] and might imply that above a certain threshold (which is yet to be identified) insulin may impair cognitive function, potentially by inducing inflammatory effects (see Sect.
5) [
144].
The results of the first multi-site phase II/III clinical trial of IN insulin for MCI and AD, conducted at 27 sites of the Alzheimer’s Therapeutic Research Institute and including 289 participants (155 of them men) between 55 and 85 years of age with a diagnosis of amnestic MCI or AD, have been recently published [
145]. The ViaNase device (Kurve Technology), which had been effectively used in previous studies on IN insulin [
138,
139,
143], proved unreliable in the first 49 participants because of problems with a newly added electronic timer. Therefore, the remaining 240 participants (designated the primary intention-to-treat population) received a daily dose of 40 IU of insulin or placebo (diluent) with the I109 Precision Olfactory Delivery device (Impel NeuroPharma) for 12 months followed by a 6-month open-label extension phase. Mean score change on the ADAS-Cog-12 [
141], evaluated at 3-month intervals, was the primary outcome measure. In contrast to the promising effects discussed above, no differences between insulin and placebo were observed in the primary measure or in other clinical (e.g., Alzheimer Disease Cooperative Study Activities of Daily Living Scale for MCI, ADLMCI [
146]) or CSF parameters (e.g., Aβ42 and Aβ40, total tau protein, tau p-181, CSF insulin concentrations). Very small reductions in hippocampal and entorhinal cortex volume were identified by MRI in the insulin- compared with the placebo-treated participants. Interestingly, in secondary analyses of the participants who used the ViaNase device, signs of improved ADAS-Cog-12 scores were observed in the insulin (
n = 23) compared with the placebo group (
n = 22) during the blinded as well as during the open-label extension phase along with increased Aβ42–Aβ40 and Aβ42 to total tau ratios as well as an insulin-induced decrease in enthorinal cortex volume. Considering that the participants were allowed to receive background therapy such as cholinesterase inhibitors or memantine, these improvements have been judged to be clinically relevant [
25].
4.2 Brain Insulin Resistance in AD and Related Memory Impairments
Given that the CNS administration (via the IN pathway) of insulin, a major factor in the control of peripheral glucose homeostasis, ameliorates cognitive function in amnestic patients, it is not surprising that impairments in systemic and brain insulin sensitivity have been found to be interrelated and that they may jointly contribute to the pathogenesis and progression of AD. “Brain insulin resistance,” defined as the failure of brain cells to respond to insulin [
24,
86], on a functional level implies that the CNS insulin signal does not effectively support cognitive processes (or the control of metabolism), and could involve downregulation or failure of insulin receptors as well as impairments of downstream signaling. Brain insulin resistance may be a cooccurrence or, potentially, a consequence of peripheral insulin resistance, which is for example in line with Fernanda de Felice’s cumulative hypothesis that the additive impact of unhealthy lifestyles (e.g., low physical activity, inadequate nutrition) eventually results in defects of brain metabolism and brain insulin signaling that trigger cognitive decline [
92]. Notably, impairments in peripheral insulin signaling in individuals with AD were suggested more than 25 years ago [
147]. Frazier and colleagues have recently come up with an inspiring account of research into brain insulin resistance, putting forward the idea that whereas brain insulin signaling may be impaired in AD, type 2 diabetes, and aging, insulin sensitivity per se may be preserved in these conditions [
103]. Indicators of brain insulin resistance have also been found in the relative absence of systemic insulin resistance (see below). As pointed out recently [
25], however, it is unclear whether insulin resistance can develop in the brain independently from systemic insulin resistance. Additionally, brain insulin resistance so far has only been determined in relation to supposedly normal insulin effects on the brain, whereas discrete functional, neurophysiological, or neuroimaging-derived criteria have not been established [
25]. A number of cognitive domains have been consistently observed to be affected in individuals with type 2 diabetes (e.g., memory, psychomotor speed, executive function, processing speed, verbal fluency, attention [
137]) and respective organ deficits include white matter lesions [
148] as well as ischemic impairments, cerebral atrophy, and cortical hypometabolism [
86]. In animal experiments, chronic hyperinsulinemia as found in obesity and diabetes was demonstrated to decrease the number of insulin receptors at the BBB [
35], thereby attenuating brain insulin uptake. Aggregation of advanced glycation end-products due to hyperglycemia likewise compromises BBB functionality [
149]. Such impairments might contribute to the increased incidence of AD in patients with metabolic impairments like diabetes that is indicated by epidemiological as well as experimental findings [e.g.,
150,
151] (for reviews see [
152,
153]), and that may have unfavorable therapeutic consequences when it comes to diabetes self-management [
154]. A recently completed clinical trial (NCT02415556) has investigated the impact of long-term administration (24 weeks and 24 weeks follow-up) of IN insulin (40 IU/day vs saline) on measures of cognition (e.g., spatial working memory, paired associate learning), daily functionality, and gait speed in adults with type 2 diabetes and controls of aged 50–85 years [
155]; its results are expected to potentially identify clinical phenotypes that predict the response to IN insulin.
Using high spatial resolution, arterial spin labeling MRI at rest and during mild hypercapnia, Frosch and colleagues [
156] compared lean controls and obese or overweight adults with and without insulin resistance and found a reduction in cerebrovascular reactivity to mild hypercapnia in obesity compared with normal weight. In the obese subjects with insulin resistance, cerebrovascular reactivity and insulin sensitivity as reflected by QUICKI values [
157] were significantly related, suggesting that impairments in cerebrovascular reactivity might precede full-blown diabetes and eventually result in a vicious circle of central and peripheral insulin resistance. Notably, individuals with systemic insulin resistance also display a decrease in hippocampal volume [
158] and hippocampal atrophy, a marker of neurodegeneration [
159]. Hyperphosphorylated tau in CSF and brain parenchyma [
160,
161] and increased deposition of Aβ [
162,
163] have been found to be associated with signs of insulin resistance in some studies. Although this and related evidence [
164] points to an association of systemic insulin resistance or type 2 diabetes and molecular symptoms of neurodegenerative diseases, many studies have failed to establish such a relationship [e.g., [
165] (for in-depth discussions of in-vivo and post-mortem studies as well as genetic risk factors, see [
25,
86]). Recent investigations that assessed brain Aβ accumulation via
11C-Pittsburgh compound B (PiB)-PET scans in 41 individuals with type 2 diabetes of the Finnish Geriatric Intervention Study to Prevent Cognitive Impairment and Disability (FINGER) likewise revealed only weak indicators of a relationship between blood markers of insulin resistance and Aβ deposition [
166].
There is some experimental support for the assumption that brain insulin resistance may contribute to the development of AD independent of systemic failures in insulin signaling (as in type 2 diabetes) [e.g.,
92,
167‐
169]. Post-mortem analyses of the brains of patients with AD have indicated decreases in messenger RNA and protein expression of insulin and insulin receptors as well as insulin-like growth factor-1 and insulin-like growth factor-2 along with signs of reduced downstream insulin signaling mechanisms that were related to disease markers of AD [
167]. Such changes may trigger negative consequences for neuronal repair, dendritic sprouting, and differentiation [
170] and impair neuronal plasticity via detrimental effects on glutamatergic and cholinergic pathways [
137,
171]. In subsequent and very sophisticated analyses of post-mortem hippocampal tissues from elderly individuals with or without AD, without a history of diabetes, indicators of dysregulation of insulin signaling pathways were detected [
168]: in a novel ex vivo stimulation paradigm, insulin signaling cascades were strongly impaired in the hippocampal tissue of patients compared with controls matched for age and sex, and these impairments were negatively related to scores of cognition and memory. In further post-mortem analyses of insulin signaling in the middle frontal gyrus cortex in 150 individuals (mean age at death, 87 years, 48% women), there were no differences between individuals with or without diabetes in IRS1 phosphorylation (pS
307IRS1/total IRS1) and Akt phosphorylation (pT
308Akt1/total Akt1); the latter was highly significantly associated with composite scores of AD pathology [
172]. (In contrast to the previous findings from the same group [
168], IRS1 serine phosphorylation was not found to be associated with cognitive AD pathology in this sample.) The concentration of insulin in the CSF of patients with AD appears to be an unresolved issue as some reports have indicated increased [
173] or, on the contrary, reduced levels [
174,
175], whereas other findings point to normal concentrations [
176,
177]; the respective contribution of potential impairments in insulin production within the CNS is an intriguing, albeit debated issue [
9,
11,
86,
103]. Deteriorations in the clearance and degradation of Aβ due to insulin resistance are discussed as a mechanism that increases the risk of AD [
178] and may be improved by insulin administration [
179‐
181]. In 3×Tg-AD mice, a rodent model of AD, IN insulin compared with placebo administration for 2 months improved measures of short-term memory (spatial learning in the Morris water maze test and novel object recognition), ameliorated depressive-like behavior (assessed by the tail suspension and the forced swim test), and decreased markers of disease pathology, i.e., tau phosphorylation in the hippocampus and frontal cortex as well as hippocampal concentrations of Aβ oligomers and 3-nitrotyrosine [
182]. These findings extend previous observations in animal experiments (e.g., [
83,
183]). Brain insulin resistance has also been assumed to be influenced by genetic factors in addition to and beyond apoE ɛ4. For example, subjects with the FTO gene polymorphism rs8050136 as well as carriers of the Gly972Arg polymorphism of IRS1 exhibit a decreased cerebrocortical response to intravenous insulin [
184,
185].
4.3 Effectiveness and Safety of Intranasal Insulin for AD
Only one study so far has presented straightforward evidence for CSF uptake of insulin after IN delivery in humans [
54]. Although studies in animals conclusively support the assumption that IN administered substances (including insulin) are readily transported to the brain compartment [
59], further experimental corroboration of the bioavailability of IN insulin, not least in patients with AD and related disorders as well as elderly individuals, would be welcome evidence for the effectiveness of IN insulin delivery. Nevertheless, respective experiments on other peptides such as oxytocin [
186] corroborate the feasibility of IN peptide administration. Considering the lack of effects on primary outcome measures in the recent multi-site phase II/III clinical trial of IN insulin for MCI and AD [
145], the currently available devices for IN drug delivery may benefit from further optimization [
187]. The device used in that trial, which relies on a liquid hydrofluoroalkane propellant to eject a metered dose of insulin through a nose tip and achieved very high adherence rates, had not been previously tested in patients with AD but proved effective in animal experiments [
59]. In this context, it should be noted that CSF increases after IN delivery of insulin [
54] and a plethora of functional effects [
62‐
65,
69,
72,
81,
114,
128,
188] in humans were observed in experiments that used a simple spray atomizer to initiate nose-to-brain transport of insulin. (Pharmacokinetic considerations notwithstanding, the same can be said of IN oxytocin [
189]). Thus, it seems worthwhile to ponder if delivery devices that include more advanced, but maybe less robust or reliable, hardware or electronic components are essential to achieve successful brain uptake of IN administered hormones. While specifically targeting the upper third of the nasal cavity to optimally reach the olfactory epithelium is certainly a worthwhile idea [
59], functional MRI assessments of regional cerebral blood flow corroborate the effectiveness of basic nasal spray devices [
190]. However, considering that advanced age [
191] and cognitive impairments including AD [
192] are associated with olfactory impairments that may be exacerbated by nasal membrane atrophy and nasal obstructions, efforts to improve the bioavailability of intranasally administered drugs are warranted. Relying on, for example, the use of nanoparticle carriers [
193], cell-penetrating peptides [
194], focused ultrasound [
195], and other absorption enhancers [
196], they have yielded promising results and might be expected to enhance the nasal uptake of insulin while maintaining the safety profile and low systemic exposure associated with IN administration.
Insulin treatment did not increase CSF insulin concentrations regardless of the administration device in the phase II/III trial, but the measurements were made at single time-points during baseline and after 12 months of administration; the authors conclude that direct (CSF- or imaging-derived) proof of the ability of an IN device to target the CNS should best be collected before its use in clinical trials [
145]. As a side note, it is worth mentioning a peculiar feature of IN insulin. All experiments in healthy participants and clinical cohorts described herein used insulin formulations (e.g., Novolin R, Humulin R, Levemir) that contain
m-cresol (meta-cresol), an excipient with a distinct “coal tar” smell that is highly noticeable (and sometimes reported to be unpleasant) during IN use. In experiments with a crossover design [e.g.,
63‐
65,
114,
128], it seems therefore mandatory to administer a diluent/carrier solution in the placebo condition to prevent premature unblinding. Although this precaution might appear of lesser relevance for parallel studies that expose participants to only one treatment [e.g.,
70,
136,
138,
139,
143], it is conceivable that the intense smell of insulin solutions elicits stronger expectancy effects than a non-odorous placebo, with potential implications for cognitive outcomes (perhaps even in respective animal studies). In the recent phase II/III trial, this potential confounder was excluded by using a diluent for the placebo [
145].
The principal effectiveness to enhance memory function of boosting brain insulin signaling by IN insulin delivery in healthy participants, but also individuals with MCI or AD has been demonstrated in the studies discussed above. While signs of a modulating effect of apoE-ε4 on the neurofunctional impact of IN insulin in patients with AD have been repeatedly found ([
134,
135,
138,
139]; see above) and animal experiments hint at potentially underlying mechanisms [
197], systematic investigations in humans are needed to clarify the relevance of apoE-ε4 in the response to IN insulin [
198], also with regard to the role of brain glucose metabolism. Experiments relying on FDG-PET in middle-aged adults at risk of developing AD revealed an association between systemic insulin resistance and lower glucose metabolism in the left temporal medial lobe that predicted impaired immediate and delayed memory performance, but did not interact with apoE-ε4 status; however, carriers of one or two ε4 alleles displayed decreased global glucose metabolism [
199]. Mice carrying the apoE ɛ4 variant in comparison with controls carrying the ɛ2 allele, which is assumed to be protective, show reduced BBB glucose transport [
200], suggesting that the higher AD risk in carriers of apoE ɛ4 may in part derive from reduced glucose transport into the brain [
201]. Against the background of these and related reports of impaired brain glucose metabolism in AD ([e.g. [
202,
203]), it might be speculated that insulin-induced enhancements of cognitive function in memory-impaired patients that occur within minutes at least in part derive from increases in cerebral glucose metabolism. However, considering that the absence of apoE ɛ4 appears to be a prerequisite for the cognitive impact of IN insulin, additional glucose-independent mechanisms are likely; it has also been argued that enhanced glucose uptake may mediate the acute effects of IN insulin whereas prolonged treatment may be necessary to induce improvements in synaptic plasticity [
204]. In recent analyses of plasma samples obtained before and after 4 months of IN insulin vs saline administration to participants with MCI [
205], favorable cognitive outcomes (ADAS-Cog) in response to the 20-IU dose of IN insulin [
143] were mirrored by changes in neuronal extracellular vesicle biomarkers of insulin resistance (pS312-IRS-1, pY-IRS-1), which are known to be increased in patients with type 2 diabetes or AD and discussed as an easily accessible marker of brain insulin resistance [
25]. This outcome, which appeared to be restricted to apoE ε4 non-carriers, suggests the engagement of the neuronal insulin cascade.
A meta-analysis of the efficacy and acceptability of antidiabetic agents (IN insulin, pioglitazone, rosiglitazone, metformin, and liraglutide) for MCI and AD that comprised 19 studies published until January 2018 found that antidiabetic treatments overall improved cognitive performance [
206]. Thus, approaches to overcome CNS insulin resistance might for example make use of the insulin-sensitizing effects of glucagon-like peptide-1 [
207] or of metformin that is routinely prescribed for type 2 diabetes [
208]. Metformin enhanced memory and decreased the concentrations of Aβ, hyperphosphorylated tau, and activated microglia in AD mouse models along with signs of improved insulin signaling in the brain [
209,
210]. On the background of promising metformin effects on memory performance in individuals with MCI but without diabetes [
211], a phase II trial (NCT04098666) in patients with MCI or AD is ongoing. While initial studies also boded well for the use of the peroxisome proliferator-activated receptor-ƴ agonist rosiglitazone [
212], subsequent clinical trials did not indicate primary endpoint improvements in AD [
213]. Moreover, a recent multi-site trial of piaglitazone in healthy participants aged 65 years or older with a high genotype-determined risk of developing cognitive impairments due to AD was terminated early for a lack of efficacy (NCT01931566 [
214]). It should also be noted that lifestyle interventions to improve dietary habits [
215] and increase physical activity [
216] hold some promise to ameliorate cognitive impairments and AD, possibly via enhancements in brain insulin signaling.
The safety profile of IN insulin has been systematically reviewed [
58] (see [
132,
217] for further reports). In 38 studies on acute IN insulin administration that included 1092 participants, no adverse events or cases of hypoglycemia were reported. Eighteen studies used long-term administration, with durations between 21 days and 9.7 years and a combined number of 832 participants. The only symptomatic case of hypoglycemia in these studies was reported after administration of a placebo spray [
218]. It was concluded that irritation of the nasal mucosa is the most commonly reported side effect, and that the IN route for insulin administration is safe and well tolerated both during acute and chronic use. These findings were corroborated in related meta-analyses [
206] and the most recent trial on IN insulin [
145] that found no indicators of clinically relevant adverse events as a result of the daily administration of 40 IU of insulin with two different administration devices.