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26.08.2020 | Original Paper

Intrastromal implantation of chicken corneal grafts into the cornea of rabbits for corneal thickening: an experimental study

verfasst von: Flavia Motta Almodin, Juliana Motta Almodin, Edna Motta Almodin, Nilma Fernandes, Paulo Ferrara, Antonio Gonçalves

Erschienen in: International Ophthalmology | Ausgabe 1/2021

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Abstract

Purpose

To evaluate the feasibility and effects of the intrastromal implantation of chemically modified corneal stroma obtained from chicken into the corneas of rabbits for corneal thickening.

Methods

Chicken corneas were cut, debrided, treated with cross-linking and implanted in an intrastromal pouch created in the cornea of 10 white New Zealand rabbits with femtosecond laser. Slit-lamp biomicroscopy and optical coherence tomography were performed immediately, 7, 30 and 90 days postoperatively. Corneas were removed at 90 days and cut in two halves. One half was sent to histological analysis for the presence of necrosis, polymorphonuclear inflammatory cells, blood vessels and fibrosis, while the other half was evaluated with transmission electron microscopy to verify tissue organization and the presence of keratocytes and inflammatory cells. Corneal thicknesses were comparatively analyzed over time with Wilcoxon test (p ≤ 0.05).

Results

The chicken grafts were incorporated into the cornea of all animals over time. Mean rabbit cornea thickness increased from 338 µm preoperatively to 538 µm (p < 0.0077) at 90 days, while mean chicken graft thickness decreased from 350 to 215 µm (p < 0.0077). No clear signs of rejection attributable to the xenograft were observed in any of the implanted eyes. However, some macroscopic and histological events were observed in some of the eyes, probably due to procedural issues during implantation.

Conclusion

The intrastromal implantation of chicken grafts was shown to be feasible and predictable to thicken the recipient rabbit cornea without apparent rejection. However, before being considered in humans, further meticulous clinical trials are required to establish the clinical utility, safety and efficacy of xenografts for the treatment of patients with advanced keratoconus.

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Titel: Intrastromal implantation of chicken corneal grafts into the cornea of rabbits for corneal thickening: an experimental study
verfasst von: Flavia Motta Almodin
Juliana Motta Almodin
Edna Motta Almodin
Nilma Fernandes
Paulo Ferrara
Antonio Gonçalves
Publikationsdatum: 26.08.2020
Verlag: Springer Netherlands
Erschienen in: International Ophthalmology / Ausgabe 1/2021
Print ISSN: 0165-5701
Elektronische ISSN: 1573-2630
DOI: https://doi.org/10.1007/s10792-020-01573-4

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Springer Medizin

Intrastromal implantation of chicken corneal grafts into the cornea of rabbits for corneal thickening: an experimental study

Abstract

Purpose

Methods

Results

Conclusion

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Ophthalmika in der Schwangerschaft

Operative Therapie und Keimnachweis bei endogener Endophthalmitis

Bakterielle endogene Endophthalmitis

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Springer Medizin

Abstract

Purpose

Methods

Results

Conclusion

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