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Pediatric Nephrology

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04.01.2019 | Review

Kidney and organoid single-cell transcriptomics: the end of the beginning

verfasst von: Parker C. Wilson, Benjamin D. Humphreys

Erschienen in: Pediatric Nephrology | Ausgabe 2/2020

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Abstract

Single-cell RNA sequencing (scRNA-seq) technologies are increasingly being applied to reveal cellular heterogeneity in kidney development and disease. In just the last year, multiple scRNA-seq datasets have been generated from kidney organoids, developing mouse and human kidney, adult kidney, and kidney cancer. The data generated enables a much deeper understanding of biological processes within and between cells. It has also elucidated unforeseen cell lineage relationships, defined the presence of off-target cell types in kidney organoids, and revealed a diverse inflammatory response in a human kidney allograft undergoing rejection. This review summarizes the recent rapid progress in scRNA-seq of the kidney and outlines future directions for single-cell technologies as applied to the kidney.

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Titel: Kidney and organoid single-cell transcriptomics: the end of the beginning
verfasst von: Parker C. Wilson
Benjamin D. Humphreys
Publikationsdatum: 04.01.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: Pediatric Nephrology / Ausgabe 2/2020
Print ISSN: 0931-041X
Elektronische ISSN: 1432-198X
DOI: https://doi.org/10.1007/s00467-018-4177-y

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Kidney and organoid single-cell transcriptomics: the end of the beginning

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