Swab transport systems have been used in the maintenance and management of specimen. In clinical settings, these STSs aid accuracy and timeliness of diagnosis, which highlights their importance. The type and quality of swab is also important in enabling efficient management of specimen, hence the development of CLSI M40-A2 standard. Following the protocols set out by this standard, we investigated and compared the recovery of
Candida spp. using the foam and flocked Sigma Transwab® swabs. The STS used in this study efficiently recovered and maintained the growth of
Candida spp. including the yeast
C. auris, which is of particular interest to clinicians due to misdiagnosis, link to nosocomial infections and drug resistance of this pathogen. While the recent study by Scansen et al. [
16] and Gandhi et al. [
17] demonstrated recovery of several
Candida spp. and other pathogenic fungi using other commercial STS based on the M40-A2 protocol, they did not investigate recovery of
C. auris. This study investigates the recovery and maintenance of different
Candida spp. including
C. auris using the M40-A2 protocol. The M40-A2 does not currently directly address the recovery of yeasts, with the exception of guidance for urine transport systems [
15]. In this study, we also found that when using the protocol for adjusting initial inocula, enumeration of the yeasts was lower than that typically determined from bacteria cells, which is possibly as a result of the characteristic differences in sizes of bacteria and yeast; bacteria are smaller in size 0.2 to 2.0 μm in diameter and 2 to 8 μm in length, while the yeasts range between 2 and 60 μm [
18,
19].
Of the
Candida spp. used within this study, only
C. auris is a known clinical isolate, and in future studies, we recommend using a panel of clinically pathogens which would better reflect the use of STS in clinical situations. However, using the reference strain cultures is in line with the M40-A2 protocol, which utilises quality control strains for testing STS. While the study by Scansen et al. [
16] suggested that foam swabs were superior to flocked swabs particularly when used in antigen-testing experiments, in our study, we did not notice any significant differences in the recovery of the
Candida spp. using both swab types. This study provides evidence of recovery of relevant yeasts including organisms of clinical importance such as the
C. albicans and
C. auris using the MWE Sigma Transwab® foam and flock swabs and offers clinical laboratories confidence to utilise these swabs in maintenance and transport of pathogenic and non-pathogenic yeasts.