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Comparative Clinical Pathology

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29.08.2018 | Original Article

Lethal effects of gold nanoparticles on protoscolices of hydatid cyst: in vitro study

verfasst von: Sara Napooni, Mohsen Arbabi, Mahdi Delavari, Hossein Hooshyar, Sima Rasti

Erschienen in: Comparative Clinical Pathology | Ausgabe 1/2019

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Abstract

Recurrence of hydatidosis may results from cyst fluid spillage during surgery. To inactivate the cyst content, different scolicidal agents have been introduced. Nevertheless, novel and more effective treatments are needed due to the associated complications. In the current study, we examined the scolicidal effects of gold nanoparticles on Echinococcus granulosus protoscoleces. Hydatid cysts of sheep liver were collected in this study. The cyst fluid containing protoscoleces was aspirated aseptically. The protoscoleces were exposed to gold nanoparticles at different concentrations (250, 500, 1000, 2000, and 4000 μg/mL). Eosin staining method was applied to determine the viability of protoscoleces at 5, 10, 20, 30, and 60 min. Moreover, cytotoxicity analysis, scanning electron microscopy (SEM) of ultrastructural changes, DNA fragmentation, were performed, and protoscoleces were studied following treatment. The significant scolicidal effects of gold nanoparticles were observed at all concentrations compared to the control group. A total of 4000 μg/mL of gold nanoparticles could destroy 76% of protoscoleces in 60 min. Cytotoxic effects of gold nanoparticle on J774 macrophage cell line, in minimum and maximum concentration (500 and 4000 μg/mL), were 1 and 11%, respectively. After treatment of protoscoleces with gold nanoparticles in different times and concentrations, considerable alteration in size, ultrastructure changes in tegument and shape of sucker, as well as DNA fragmentation of parasite were seen. Based on the results, gold nanoparticles could exert scolicidal effects on hydatid cyst protoscoleces; therefore, they can be applied in hydatid cyst treatment.

Abdel-Baki AAS, Almalki E, Mansour L, Al-Quarishy S (2016) In vitro Scolicidal effects of Salvadora persica root extract against Protoscolices of Echinococcus granulosus. Korean J Parasitol 54(1):61–66CrossRef

Adas G, Arikan S, Kemik O, Oner A, Sahip N, Karatepe O (2009) Use of albendazole sulfoxide, albendazole sulfone, and combined solutions as scolicidal agents on hydatid cysts (in vitro study). World J Gastroenterol 15(1):112–116CrossRef

Allahverdiyev AM, Abamor ES, Bagirova M, Ustundag CB, Kaya C, Kaya F, Rafailovich M (2011) Antileishmanial effect of silver nanoparticles and their enhanced antiparasitic activity under ultraviolet light. Int J Nanomedicine 6:2705–2714CrossRef

Alvarez I, Gutierrez G, Barrandeguy M, Trono K (2010) Immuno chromatographic lateral flow test for detection of antibodies to equine infectious anemia virus. J Virol Methods 167(2):152–157CrossRef

Barabadi H, Honary S, Mohammadi MA, Ahmadpour E, Rahimi MT, Alizadeh A, Naghibi F, Saravanan M (2017) Green chemical synthesis of gold nanoparticles by using Penicilliumaculeatum and their scolicidal activity against hydatid cyst protoscolices of Echinococcus granulosus. Environ Sci Pollut Res Int 24(6):5800–5810CrossRef

Bari SU, Arif SH, Malik AA, Khaja AR, Dass TA, Naikoo ZA (2011) Role of albendazole in the management of hydatid cyst liver. Saudi J Gastroenterol 17(5):343–347CrossRef

Barzinji AKR, Mothana RA, Nasher AK (2009) Effect of leaf extracts of Dendrosicyossocotrana and Jatropha unicostata on the viability of Echinococcusgranulosusprotoscoleces. EurAsian J BioSci 3:122–129CrossRef

Beheshti N, Soflaei S, Shakibaie M, Yazdi MH, Ghaffarifar F, Dalimi A, Shahverdi AR (2013) Efficacy of biogenic selenium nanoparticles against Leishmania major: in vitro and in vivo studies. J Trace Elem Med Biol 27(3):203–207CrossRef

Berners-Price SJ, Filipovska A (2011) Gold compounds as therapeutic agents for human diseases. Metallomics 3(9):863–873CrossRef

Budke CM, Casulli A, Kern P, Budke CM, Casulli A, Kern P, Vuitton DA (2017) Cystic and alveolar echinococcosis: successes and continuing challenges. PLoS Negl Trop Dis 11(4):e000 5477CrossRef

Caglar R, Yuzbasioglu MF, Bulbuloglu E, Gul M, Ezberci F, Kale IT (2008) In vitro effectiveness of different chemical agents on scolices of hydatid cyst. J Investig Surg 21(2):71–75CrossRef

da Silva AM (2010) Human echinococcosis: a neglected disease Gastroenterol Res Pract Article ID 583297

Das S, Roy P, Mondal S, Bera T, Mukherjee A (2013) One pot synthesis of gold nanoparticles and application in chemotherapy of wild and resistant type visceral leishmaniasis. Colloids Surf B: Biointerfaces 1(107):27–34CrossRef

Faa G, Gerosa C, Fanni D, Lachowicz JI, Nurchi VM (2017) Gold - old drug with new potentials. Curr Med Chem

Fakhar M, Chabra A, Rahimi-Esboei B, Rezaei F (2015) In vitro protoscolicidal effects of fungal chitosan isolated from Penicillium waksmanii and Penicillium citrinum. J Parasit Dis 39(2):162–167CrossRef

FasihiHarandi M, Budke CM, Rostami S (2012) The monetary burden of cystic echinococcosis in Iran. PLoS Negl Trop Dis 6(11):e1915CrossRef

Gholami SH, Rahimi-Esboei B, Ebrahimzadeh MA, Pourhajibagher M (2013) In vitro effect of Sambucusebulus on scolices of hydatid cysts. Eur Rev Med Pharmacol Sci 17(13):1760–1765PubMed

Haghani A, Roozitalab A, Safi SN (2014) Low scolicidal effect of Ocimumbacilicum and Allium cepa on protoccoleces of hydatid cyst: an in vitro study. Comp Clin Pathol 23:847–853CrossRef

Khan R, Zakir M, Afaq SH, Latif A, Khan AU (2010) Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strains. J Infect Dev Ctries 4(5):292–300CrossRef

Kilicoglu B, Kismet K, Kilicoglu SS, Erel S, Gencay O, Sorkun K, Erdemli E, Akhan O, Akkus MA, Sayek I (2006) Effects of honey as a scolicidal agent on the hepatobiliary system. World J Gastroenterol: WJG 149(13):2085–2088CrossRef

Kumar KP, Murmu S, Saha S, Tandon V, Acharya K (2014) Anthelmintic efficacy of gold nanoparticles derived from a Phytopathogenic fungus, Nigrospora oryzae. PLoS One 9(1):e84693CrossRef

Kumar RC, Ray P, Datta DP, Bansal G, Angov E, Kumar N (2015) Nanovaccines for malaria using plasmodium falciparum antigen Pfs25 attached gold nanoparticles. Vaccine 33(39):5064–5071CrossRef

Lam J, Herant M, Dembo M, Heinrich V (2009) Baseline mechanical characterization of J774 macrophages. Biophys J 96(1):248–254CrossRef

Lashkarizadeh MR, Asgaripour K, Dezaki ES, FasihiHarandi M (2015) Comparison of Scolicidal effects of Amphotricin B, silver nanoparticles, and Foeniculumvulgare mill on hydatid cysts Protoscoleces. Iran J Parasitol 10(2):206–212PubMedPubMedCentral

Lima E, Guerra R, Lara V, Guzmán A (2013) Gold nanoparticles as efficient antimicrobial agents for Escherichia coli and Salmonella typhi. Chem Cent J 7(11):1–7

Lv H, Jiang Y, Liao M, Sun H, Zhang S, Peng X (2013) In vitro and in vivo treatments of Echinococcus granulosus with Huaier aqueous extract and albendazole liposome. Parasitol Res 112(1):193–198CrossRef

Mahmoudvand H, FasihiHarandi M, Shakibaie M, Aflatoonian MR, Makki MS, Jahanbakhsh S (2014a) Scolicidal effects of biogenic selenium nanoparticles against protoscolices of hydatid cysts. Int J Surg 12(5):399–403CrossRef

Mahmoudvand H, Sharififar F, Dezaki ES, Ezatpour B, Jahanbakhsh S, FasihiHarandi M (2014b) Protoscolecidal effect of Berberis vulgaris root extract and its main compound, berberine in cystic echinococcosis. Iran J Parasitol 9(4):26–34

Malik AA, Bari SU, Amin R, Jan M (2010) Surgical management of complicated hydatid cysts of the liver. World J Gastrointest Surg 2:78–84CrossRef

Mayelifar K, Taheri K, Rajabi O, Sazgarnia A (2015) Ultraviolet B efficacy in improving antileishmanial effects of silver nanoparticles. Iran J Basic Med Sci 18(7):677–683PubMedPubMedCentral

Mikami Y, Dhakshinamoorthy A, Alvaro M, García H (2013) Catalytic activity of unsupported gold nanoparticles. Catal Sci Technol 3:58–69CrossRef

Moazeni M, Nazer A (2010) In vitro effectiveness of garlic (Allium sativum) extract on scolices of hydatid cyst. World J Surgy 34(11):2677–2681CrossRef

Moazeni M, Nazer A (2011) In vitro lethal effect of Zingiber officinale R. On protoscolices of hydatid cyst from sheep liver. Microbiol Res 2:91–94CrossRef

Moazeni M, Roozitalab A (2012) High scolicidal effect of Zatariamulti¬flora on protoccoleces of hydatid cyst: an in vitro study. Comp Clin Pathol 21:99–104CrossRef

Moazeni M, Saharkhiz MJ, Hosseinic AA (2012) In vitro lethal effect of ajowan (Trachyspermumammi L.) essential oil on hydatid cyst protoscoleces. Vet Parasitol 187(1–2):203–208CrossRef

Mohamed MM, Fouad SA, Elshoky HA, Mohammed GM, Salaheldin TA (2017) Antibacterial effect of gold nanoparticles against Corynebacterium pseudotuberculosis. Int J Vet Sci Med 5:23–29CrossRef

Moro PL, Schantz PM (2006) Echinococcosis: historical landmarks and progress in research and control. Ann Trop Med Parasitol 100(8):703–714CrossRef

Moro P, Schantz PM (2009) Echinococcosis: a review. Int J Infect Dis 13(2):125–133CrossRef

Naseri M, Akbarzadeh A, Spotin A, Akbari NA, Mahami-Oskouei M, Ahmadpour E (2016) Scolicidal and apoptotic activities of albendazole sulfoxide and albendazole sulfoxide-loaded PLGA-PEG as a novel nanopolymeric particle against Echinococcusgranulosusprotoscoleces. Parasitol Res 15(12):4595–4603CrossRef

Rahimi MT, Ahmadpour E, RahimiEsboei B, Spotin A, KohansalKoshki MH, Alizadeh A, Honary S, Barabadi H, Mohammadi AM (2015) Scolicidal activity of biosynthesized silver nanoparticles against Echinococcus granulosus protoscolices. Int J Surg 19:128–133CrossRef

Said DE, Elsamad LM, Gohar YM (2012) Validity of silver, chitosan, and curcumin nanoparticles as anti-Giardia agents. Parasitol Res 111(2):545–554CrossRef

Santhoshkumar T, Rahuman AA, Rajakumar G, Marimuthu S, Bagavan A, Jayaseelan C, Zahir AA, Elango G, Kamaraj C (2011) Synthesis of silver nanoparticles using Nelumbonucifera leaf extract and its larvicidal activity against malaria and filariasis vectors. Parasitol Res 108(3):693–702CrossRef

Shahnazi M, Badakhsh F, Azadmehr A, Saraei M, Alipour M, Shahnazi M, Jamshidi M (2014) Study of protoscolicidal effects of hypertonic glucose on protoscolices of hydatid cyst at different concentrations and exposure times. Int Sch Res Notices 314502:1–5

Shahnazi M, Azadmehr A, Latifi R, Hajiaghaee R, Saraei, Alipour M (2016) In vitro protoscolicidal effects of various concentrations of Ziziphoratenuior L. extract at different exposure times. Avicenna J Phytomed 6(4):376–382PubMedPubMedCentral

Shamaila S, Zafar N, Riaz S, Sharif R, Nazir J, Naseem S (2016) Gold nanoparticles: an efficient antimicrobial agent against enteric bacterial human pathogen. Nanomaterials 6(4):2–10CrossRef

Smyth JD, Barret NJ (1980) Procedures for testing the viability of human hydatid cyst following surgical removal, especially after chemotherapy. Trans R Soc Trop Med Hyg 74(5):649–652CrossRef

Spicher M, Roethlisberger C, Lany C, Stadelmann B, Keiser J, Ortega LM, Gottstein B, Hemphill A (2008) In vitro and in vivo treatments of Echinococcusprotoscoleces and metacestodes with artemisinin and artemisinin derivatives. Antimicrob Agents Chemother 52(9):3447–3450CrossRef

Sugimoto Y, Pou P, Abe M, Jelinek P, Pérez R, Morita S, Custance O (2007) Chemical identification of individual surface atoms by atomic force microscopy. Nature 446(7131):64–67CrossRef

Tamarozzi F, Vuitton L, Brunetti E, Vuitton DA, Koch S (2014) Non-surgical and non-chemical attempts to treat echinococcosis: do they work? Parasite 21(75):1–13

Topcu O, Aydin C, Arici S, Duman M, Koyuncu A, Sen M (2006) The effects of various scolicidal agents on the hepatopancreatic biliary system. Visc Med 22:185–190CrossRef

Tsai CY, Shiau AL, Chen SY, Chen YH, Cheng PC, Chang MY, Chen DH, Chou CH, Wang CR, Wu CL (2007) Amelioration of collagen induced arthritis in rats by nanogold. Arthritis Rheum 56(2):544–554CrossRef

Vinoj G, Pati R, Sonawane A, Vaseeharana B (2015) In vitro cytotoxic effects of gold nanoparticles coated with functional acyl Homoserine lactone lactonase protein from Bacillus licheniformis and their Antibiofilm activity against Proteus species. Antimicrob Agents Chemother 59(2):763–771CrossRef

Wang S, Yang T, Zhang X, Xia J, Guo J, Wang X, Hou J, Zhang H, Chen X, Wu X (2016) Construction of in vivo fluorescent imaging of Echinococcus granulosus in a mouse model. Korean J Parasitol 54(3):291–299CrossRef

Want MY, Islammudin M, Chouhan G, Ozbak HA, Hemeg HA, Chattopadhyay AP, Afrin A (2017) Nanoliposomal artemisinin for the treatment of murine visceral leishmaniasis. Int J Nanomedicine 20(12):2189–2204CrossRef

Zhang S, Zhou Y, Su L, Zhang X, Wang H, Liu B (2017) In vivo evaluation of the efficacy of combined Albedazole-IFN-α treatment for cystic echinococcosis in mice. Parasitol Res 116(2):735–742CrossRef

Titel: Lethal effects of gold nanoparticles on protoscolices of hydatid cyst: in vitro study
verfasst von: Sara Napooni
Mohsen Arbabi
Mahdi Delavari
Hossein Hooshyar
Sima Rasti
Publikationsdatum: 29.08.2018
Verlag: Springer London
Erschienen in: Comparative Clinical Pathology / Ausgabe 1/2019
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-018-2807-1

Springer Medizin

Lethal effects of gold nanoparticles on protoscolices of hydatid cyst: in vitro study

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

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