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Knee Surgery, Sports Traumatology, Arthroscopy

Erschienen in:

01.12.2012 | Experimental Study

One-step osteochondral repair with cartilage fragments in a composite scaffold

verfasst von: A. Marmotti, M. Bruzzone, D. E. Bonasia, F. Castoldi, R. Rossi, L. Piras, A. Maiello, C. Realmuto, G. M. Peretti

Erschienen in: Knee Surgery, Sports Traumatology, Arthroscopy | Ausgabe 12/2012

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Abstract

Purpose

This study proposes a single-step therapeutic approach for osteochondral defects using autologous cartilage fragments loaded onto a scaffold composed of a hyaluronic acid (HA) derivative, human fibrin glue (FG) and autologous platelet-rich-plasma (PRP), in a rabbit model. The aim is to demonstrate the in vitro outgrowth of chondrocytes from cartilage fragments and the in vivo formation of a functional repair tissue.

Methods

In vitro: minced articular cartilage was loaded onto two different types of scaffold (paste or membrane) according to two different HA preparations (injectable HA-derivative or HA-derivative felt). In vivo: trochlear osteochondral defects were created in 50 adult rabbits, which were then assigned to 5 different treatment groups: cartilage fragments loaded onto membrane scaffolds with FG (Group 1) or without FG (Group 2); membrane scaffolds alone with FG (Group 3) or without FG (Group 4); empty defects (Group 5). Membrane scaffolds were used “in vivo” for simpler preparation and better adhesive properties. Repair processes were evaluated histologically and by immunohistochemistry at 1, 3, and 6 months.

Results

An in vitro time-dependent cell outgrowth from cartilage fragments was observed with both types of scaffolds. At 6 months, in vivo, cartilage fragment-loaded scaffolds induced significantly better repair tissue than the scaffold alone using histological scoring. Repair in Group 2 was superior to that in any of the control groups (p < 0.05).

Conclusion

Autologous cartilage fragments loaded onto an HA felt/FG/PRP-scaffold provided an efficient cell source, and allowed for an improvement of the repair process of ostechondral defects in a rabbit model. Human FG, however, hampered the rabbit healing process. These results may have clinical relevance as they show the potential of a novel one-stage repair technique for osteochondral defects.

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Titel: One-step osteochondral repair with cartilage fragments in a composite scaffold
verfasst von: A. Marmotti
M. Bruzzone
D. E. Bonasia
F. Castoldi
R. Rossi
L. Piras
A. Maiello
C. Realmuto
G. M. Peretti
Publikationsdatum: 01.12.2012
Verlag: Springer-Verlag
Erschienen in: Knee Surgery, Sports Traumatology, Arthroscopy / Ausgabe 12/2012
Print ISSN: 0942-2056
Elektronische ISSN: 1433-7347
DOI: https://doi.org/10.1007/s00167-012-1920-y

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One-step osteochondral repair with cartilage fragments in a composite scaffold