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Aesthetic Plastic Surgery

Erschienen in:

31.10.2022 | Original Article

Strategies to Improve AFT Volume Retention After Fat Grafting

verfasst von: Meiling Liu, Yujia Shang, Na Liu, Yonghuan Zhen, Youbai Chen, Yang An

Erschienen in: Aesthetic Plastic Surgery | Ausgabe 2/2023

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Abstract

Background

Autologous fat grafting has gained increasing popularity used in plastic surgery as a strategy to improve functional and aesthetic outcome. However, variable augmentation results have concerned surgeons in that volume loss of grafted fat reported fluctuates unsteadily.

Aim

An optimal technique that clinically maximizes the long-term survival rate of transplantation is in urgent need to be identified.

Method

The PubMed/MEDLINE database was queried to search for animal and human studies published through March of 2022 with search terms related to adipose grafting encompassing liposuction, adipose graft viability, processing technique, adipose-derived stem cell, SVF and others.

Results

45 in vivo studies met inclusion criteria. The principal of ideal processing technique is effective purification of fat and protection of tissue viability, such as gauze rolling and washing-filtration devices. Cell-assisted lipotransfer including SVF, SVF-gel and ADSCs significantly promotes graft retention via differentiation potential and paracrine manner. ADSCs induce polarization of macrophages to regulate inflammatory response, mediate extracellular matrix remodeling and promote endothelial cell migration and sprouting, and differentiate into adipocytes to replace necrotic cells, providing powerful evidence for the benefits and efficacy of cell-assisted lipotransfer.

Conclusion

Based on the current evidence, the best strategy can not be decided. Cell-assisted lipotransfer has great potential for use in regenerative medicine. But so far mechanically prepared SVF-gel is conducive to clinical promotion. PRP as endogenous growth factor sustained-release material shows great feasibility.

Level of Evidence IV

This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

Vyas KS, Vasconez HC, Morrison S, Mogni B, Linton S, Hockensmith L, Kabir T, Zielins E, Najor A, Bakri K, Mardini S (2020) Fat Graft enrichment strategies: a systematic review. Plast Reconstr Surg 145:827–841PubMedCrossRef

Li J, Gao J, Cha P, Chang Q, Liao Y, Liu C, Li K, Lu F (2013) Supplementing fat grafts with adipose stromal cells for cosmetic facial contouring. Dermatol Surg 39:449–456PubMedCrossRef

Missana MC, Laurent I, Barreau L, Balleyguier C (2007) Autologous fat transfer in reconstructive breast surgery: indications, technique and results. Eur J Surg Oncol (EJSO) 33(6):685–690. https://doi.org/10.1016/j.ejso.2006.12.002CrossRefPubMed

Simonacci F, Bertozzi N, Grieco MP, Grignaffini E, Raposio E (2017) Procedure, applications, and outcomes of autologous fat grafting. Ann Med Surg 20:49–60. https://doi.org/10.1016/j.amsu.2017.06.059CrossRef

Strong AL, Rubin JP, Kozlow JH, Cederna PS (2019) Fat grafting for the treatment of scleroderma. Plast Reconstr Surg 144:1498–1507PubMedCrossRef

Tabit CJ, Slack GC, Fan K, Wan DC, Bradley JP (2012) Fat grafting versus adipose-derived stem cell therapy: distinguishing indications, techniques, and outcomes. Aesth Plast Surg 36(3):704–713. https://doi.org/10.1007/s00266-011-9835-4CrossRef

Wang GH, Zhao JF, Xue HY, Li D (2019) Facial aesthetic fat graft retention rates after filtration, centrifugation, or sedimentation processing techniques measured using three-dimensional surface imaging devices. Chin Med J 132(01):69–77PubMedPubMedCentralCrossRef

Gontijo-de-Amorim NF, Charles-de-Sá L, Rigotti G (2017) Mechanical supplementation with the stromal vascular fraction yields improved volume retention in facial lipotransfer: a 1-year comparative study. Aesthet Surg J 37:975–985PubMedCrossRef

Zhang K, Liu F, Zhang Y, Huang X, Tang M, Hou Y, Lv Q, Jin D, Li Y, Kong L (2020) Mechanical vibration-extracted stromal vascular fraction improves volume retention after autologous fat grafting. Plast Reconstr Surg 146:1275–1284PubMedCrossRef

10.

Shridharani SM, Broyles JM, Matarasso A (2014) Liposuction devices: technology update. Med Dev (Auckland, NZ). 7:241

11.

Molitor M, Trávníčková M, Měšťák O, Christodoulou P, Sedlář A, Bačáková L, Lucchina S (2021) The influence of low- and high-negative-pressure liposuction and different harvesting sites on the viability and yield of adipocytes and other nucleated cells. Aesthetic Plast Surg 45:2952–2970PubMedCrossRef

12.

Lee JH, Kirkham JC, McCormack MC, Nicholls AM, Randolph MA, Austen WG Jr (2013) The effect of pressure and shear on autologous fat grafting. Plast Reconstr Surg 131(5):1125–1136PubMedCrossRef

13.

Mojallal A, Auxenfans C, Lequeux C, Braye F, Damour O (2008) Influence of negative pressure when harvesting adipose tissue on cell yield of the stromal–vascular fraction. BioMed Mater Eng 18(4–5):193–197PubMed

14.

Cucchiani R, Corrales L (2016) The effects of fat harvesting and preparation, air exposure, obesity, and stem cell enrichment on adipocyte viability prior to graft transplantation. Aesthet Surg J 36:1164–1173PubMedCrossRef

15.

Chen YW, Wang JR, Liao X, Li SH, Xiao LL, Cheng B, Xie GH, Song JX, Liu HW (2017) Effect of suction pressures on cell yield and functionality of the adipose-derived stromal vascular fraction. J Plast Reconstr Aesthetic Surg 70(2):257–66CrossRef

16.

Vazquez OA, Markowitz MI, Becker H (2020) Fat graft size: relationship between cannula and needle diameters. Cureus 12:e7598PubMedPubMedCentral

17.

Erdim M, Tezel E, Numanoglu A, Sav A (2009) The effects of the size of liposuction cannula on adipocyte survival and the optimum temperature for fat graft storage: an experimental study. J Plast Reconstr Aesthetic Surg 62(9):1210–1214CrossRef

18.

Kirkham JC, Lee JH, Medina MA, McCormack MC, Randolph MA, Austen WG (2012) The impact of liposuction cannula size on adipocyte viability. Ann Plast Surg 69(4):479–481. https://doi.org/10.1097/SAP.0b013e31824a459fCrossRefPubMed

19.

Shiffman MA, Mirrafati S (2001) Fat transfer techniques: the effect of harvest and transfer methods on adipocyte viability and review of the literature. Dermatol Surg 27(9):819–826PubMed

20.

Eto H, Kato H, Suga H, Aoi N, Doi K, Kuno S, Yoshimura K (2012) The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes. Plast Reconstr Surg 129(5):1081–1092. https://doi.org/10.1097/PRS.0b013e31824a2b19CrossRefPubMed

21.

Carpaneda CA, Ribeiro MT (1993) Study of the histologic alterations and viability of the adipose graft in humans. Aesthetic Plast Surg 17(1):43–47. https://doi.org/10.1007/BF00455048CrossRefPubMed

22.

Alharbi Z, Opländer C, Almakadi S, Fritz A, Vogt M, Pallua N (2013) Conventional vs. micro-fat harvesting: how fat harvesting technique affects tissue-engineering approaches using adipose tissue-derived stem/stromal cells. J Plast Reconstr Aesthetic Surg 66(9):1271–1278CrossRef

23.

Flynn TC, Coleman WP, Field LM, Klein JA, Hanke WC (2000) History of liposuction. Dermatol Surg 26(6):515–520. https://doi.org/10.1046/j.1524-4725.2000.00066.xCrossRefPubMed

24.

Lillis PJ (1990) The tumescent technique for liposuction surgery. Dermatol Clin 8(3):439–450. https://doi.org/10.1016/S0733-8635(18)30475-3CrossRefPubMed

25.

Coleman SR (1995) Long-term survival of fat transplants: controlled demonstrations. Aesthetic Plast Surg 19(5):421–425. https://doi.org/10.1007/BF00453875CrossRefPubMed

26.

Zhu M, Cohen SR, Hicok KC, Shanahan RK, Strem BM, Yu JC, Arm DM, Fraser JK (2013) Comparison of three different fat graft preparation methods: gravity separation, centrifugation, and simultaneous washing with filtration in a closed system. Plast Reconstr Surg 131:873–880PubMedCrossRef

27.

Bourne DA, Bliley J, James I, Donnenberg AD, Donnenberg VS (2021) Changing the paradigm of craniofacial reconstruction: a prospective clinical trial of autologous fat transfer for craniofacial deformities. Ann Surg 273:1004–1011PubMedCrossRef

28.

Rongwei W, Yang X, Jin X, Haibin L, Jia Z, Li B, Jiang H, Qi Z (2018) Three-dimensional volumetric analysis of 3 fat-processing techniques for facial fat grafting: a randomized clinical trial. JAMA Facial Plast Surg 20(3):222–229. https://doi.org/10.1001/jamafacial.2017.2002CrossRef

29.

Sarfati I (2017) A prospective randomized study comparing centrifugation and sedimentation for fat grafting in breast reconstruction. J Plast Reconstr Aesthet Surg 70:1218–1228PubMedCrossRef

30.

Mestak O, Sukop A, Hsueh YS, Molitor M, Mestak J, Matejovska J, Zarubova L (2014) Centrifugation versus puregraft for fatgrafting to the breast after breast-conserving therapy. World J Surg Oncol 12(1):178. https://doi.org/10.1186/1477-7819-12-178CrossRefPubMedPubMedCentral

31.

Asilian A, Siadat AH, Iraji R (2014) Comparison of fat maintenance in the face with centrifuge versus filtered and washed fat. J Res Med Sci Official J Isfahan Univ Med Sci 19(6):556–561

32.

Gerth DJ, King B, Rabach L, Glasgold RA, Glasgold MJ (2014) Long-term volumetric retention of autologous fat grafting processed with closed-membrane filtration. Aesthetic Surg J 34(7):985–994. https://doi.org/10.1177/1090820X14542649CrossRef

33.

Gentile P, Orlandi A, Scioli MG, Di Pasquali C, Bocchini I, Curcio CB, Floris M, Fiaschetti V, Floris R, Cervelli V (2012) A comparative translational study: the combined use of enhanced stromal vascular fraction and platelet-rich plasma improves fat grafting maintenance in breast reconstruction. Stem Cells Transl Med 1(4):341–351. https://doi.org/10.5966/sctm.2011-0065CrossRefPubMedPubMedCentral

34.

Botti G, Pascali M, Botti C, Bodog F, Cervelli V (2011) A Clinical Trial in facial fat grafting: filtered and washed versus centrifuged fat. Plast Reconstr Surg 127:2464–2473PubMedCrossRef

35.

Fan P, Fang M, Li J, Solari MG, Wu D, Tan W, Wang Y, Yang X, Lei S (2021) A novel fat making strategy with adipose-derived progenitor cell-enriched fat improves fat graft survival. Aesthetic Surg J 41(9):1228–1236CrossRef

36.

Canizares OJ, Thomson JE, Allen RJJ, Davidson EH, Tutela JP, Saadeh PB, Warren SM, Hazen A (2017) The effect of processing technique on fat graft survival. Plast Reconstr Surg 140:933–943PubMedCrossRef

37.

Yin S, Luan J, Fu S, Zhuang Q (2016) Is centrifugation necessary for processing lipoaspirate harvested via water-jet force assisted technique before grafting? Evidence of lipoaspirate concentration with enhanced fat graft survival. Ann Plast Surg 77:477–484PubMedCrossRef

38.

Qiu L, Su Y, Zhang D, Song Y, Liu B, Yu Z, Guo S, Yi C (2016) Identification of the centrifuged lipoaspirate fractions suitable for postgrafting survival. Plast Reconstr Surg 137:67e–76ePubMedCrossRef

39.

Salinas HM, Broelsch GF, Fernandes JR, McCormack MC, Meppelink AM, Randolph MA, Colwell AS, Austen WGJ (2014) Comparative analysis of processing methods in fat grafting. Plast Reconstr Surg 134:675–683PubMedCrossRef

40.

Ansorge H, Garza JR, McCormack MC, Leamy P, Roesch S, Barere A, Connor J (2014) Autologous fat processing via the revolve system: quality and quantity of fat retention evaluated in an animal model. Aesthetic Surg J 34(3):438–47CrossRef

41.

Fisher C, Grahovac TL, Schafer ME, Shippert RD, Marra KG, Rubin JP (2013) Comparison of harvest and processing techniques for fat grafting and adipose stem cell isolation. Plast Reconstr Surg 132:351–361PubMedCrossRef

42.

Minn KW, Min KH, Chang H, Kim S, Heo EJ (2010) Effects of fat preparation methods on the viabilities of autologous fat grafts. Aesthetic Plast Surg 34(5):626–631. https://doi.org/10.1007/s00266-010-9525-7CrossRefPubMed

43.

Condé-Green A, de Amorim NF, Pitanguy I (2010) Influence of decantation, washing and centrifugation on adipocyte and mesenchymal stem cell content of aspirated adipose tissue: a comparative study. J Plast Reconstr Aesthet Surg 63(8):1375–1381PubMedCrossRef

44.

Cao Z, Li H, Wang ZH, Liang XQ (2021) High-density fat grafting assisted stromal vascular fraction gel in facial deformities. J Craniofacial Surg 33(1):108–111. https://doi.org/10.1097/SCS.0000000000008038CrossRef

45.

Allen RJJ, Canizares O, Scharf CL, Paek GK, Nguyen PD, Thanik VD, Warren SM, Saadeh PB, Coleman SR, Hazen A (2009) Spinning into control: centrifugation creates an optimal density for fat grafting. Plast Reconstr Surg 124:35–36

46.

Condé-Green A, Iwen W, Graham I, Chae JJ, Drachenberg CB, Singh DP, Holton L, Slezak S, Elisseeff J (2013) Comparison of 3 techniques of fat grafting and cell-supplemented lipotransfer in athymic rats. Aesthetic Surg J 33(5):713–721. https://doi.org/10.1177/1090820X13487371CrossRef

47.

Hoareau L, Bencharif K, Girard AC, Gence L, Delarue P, Hulard O, Festy F, Roche R (2013) Effect of centrifugation and washing on adipose graft viability: a new method to improve graft efficiency. J Plast Reconstr Aesthet Surg 66(5):712–9PubMedCrossRef

48.

Ferraro GA, De Francesco F, Tirino V, Cataldo C, Rossano F, Nicoletti G, D’Andrea F (2011) Effects of a new centrifugation method on adipose cell viability for autologous fat grafting. Aesthetic Plast Surg 35(3):341–348PubMedCrossRef

49.

Pfaff M, Wu W, Zellner E, Steinbacher DM (2014) Processing technique for lipofilling influences adipose-derived stem cell concentration and cell viability in lipoaspirate. Aesthetic Plast Surg 38(1):224–229PubMedCrossRef

50.

Gonzalez AM, Lobocki C, Kelly CP, Jackson IT (2007) An alternative method for harvest and processing fat grafts: an in vitro study of cell viability and survival. Plast Reconstr Surg 120(1):285–294PubMedCrossRef

51.

Hu S, Zhang H, Feng Y, Yang Y, Han X, Han X, Zhong Y, Shi J (2007) Introduction of an easy technique for purification and injection of autogenous free fat parcels in correcting of facial contour deformities. Ann Plast Surg 58(6):602–607PubMedCrossRef

52.

Valmadrid AC, Kaoutzanis C, Wormer BA, Farinas AF, Wang L, Al Kassis S, Perdikis G, Braun SA, Higdon KK (2020) Comparison of telfa rolling and a closed washing system for autologous fat processing techniques in postmastectomy breast reconstruction. Plast Reconstr Surg 146:486–497PubMedCrossRef

53.

Fang C, Patel P, Li H, Huang LT, Wan H, Collins S, Connell TL, Xu H (2020) Physical, biochemical, and biologic properties of fat graft processed via different methods. Plast Reconstr Surg Glob Open 8:e3010PubMedPubMedCentral

54.

Pallua N, Pulsfort AK, Suschek C, Wolter TP (2009) Content of the growth factors bFGF, IGF-1, VEGF, and PDGF-BB in freshly harvested lipoaspirate after centrifugation and incubation. Plast Reconstr Surg 123:826–833PubMedCrossRef

55.

Smith P, Adams WPJ, Lipschitz AH, Chau B, Sorokin E, Rohrich RJ, Brown SA (2006) Autologous human fat grafting: effect of harvesting and preparation techniques on adipocyte graft survival. Plast Reconstr Surg 117:1836–1844PubMedCrossRef

56.

Vezzani B, Shaw I, Lesme H, Yong L, Khan N, Tremolada C, Péault B (2018) Higher pericyte content and secretory activity of microfragmented human adipose tissue compared to enzymatically derived stromal vascular fraction. Stem Cells Transl Med 7:876–886PubMedPubMedCentralCrossRef

57.

Vezzani B, Gomez-Salazar M, Casamitjana J, Tremolada C, Péault B (2019) Human adipose tissue micro-fragmentation for cell phenotyping and secretome characterization. JoVE 152:e60117

58.

Ceserani V, Ferri A, Berenzi A, Benetti A, Ciusani E, Pascucci L, Bazzucchi C, Coccè V, Bonomi A, Pessina A, Ghezzi E (2016) Angiogenic and anti-inflammatory properties of micro-fragmented fat tissue and its derived mesenchymal stromal cells. Vasc Cell 8(1):1–2CrossRef

59.

Guo B, Sawkulycz X, Heidari N, Rogers R, Liu D, Slevin M (2021) Characterisation of novel angiogenic and potent anti-inflammatory effects of micro-fragmented adipose tissue. Int J Mol Sci 22(6):3271PubMedPubMedCentralCrossRef

60.

Vinet-Jones H, Darr DK (2020) Clinical use of autologous micro-fragmented fat progressively restores pain and function in shoulder osteoarthritis. Regener Med 15(10):2153–2161CrossRef

61.

Malanga GA, Chirichella PS, Hogaboom NS, Capella T (2021) Clinical evaluation of micro-fragmented adipose tissue as a treatment option for patients with meniscus tears with osteoarthritis: a prospective pilot study. Int Orthopaed 45(2):473–480CrossRef

62.

Yoshimura K, Sato K, Aoi N, Kurita M, Hirohi T, Harii K (2020) Cell-Assisted lipotransfer for cosmetic breast augmentation: supportive use of adipose-derived stem/stromal cells. Aesthetic Plast Surg 44:1258–1265PubMedCrossRef

63.

Yoshimura K, Asano Y, Aoi N, Kurita M, Oshima Y, Sato K, Inoue K, Suga H, Eto H, Kato H, Harii K (2010) Progenitor-enriched adipose tissue transplantation as rescue for breast implant complications. Breast J 16:169–175PubMedCrossRef

64.

Yoshimura K, Sato K, Aoi N, Kurita M, Inoue K, Suga H, Eto H, Kato H, Hirohi T, Harii K (2008) Cell-assisted lipotransfer for facial lipoatrophy: efficacy of clinical use of adipose-derived stem cells. Dermatol Surg 34(9):1178–85PubMed

65.

Andia I, Maffulli N, Burgos-Alonso N (2019) Stromal vascular fraction technologies and clinical applications. Expert Opinion Biol Ther 19(12):1289–1305. https://doi.org/10.1080/14712598.2019.1671970CrossRef

66.

Gentile P, Casella D, Palma E, Calabrese C (2019) Engineered Fat graft enhanced with adipose-derived stromal vascular fraction cells for regenerative medicine: clinical, histological and instrumental evaluation in breast reconstruction. J Clin Med 8(4):504. https://doi.org/10.3390/jcm8040504CrossRefPubMedPubMedCentral

67.

Jeon HJ, Choi DH, Lee JH, Lee JS, Lee J, Park HY, Yang JD (2021) A prospective study of the efficacy of cell-assisted lipotransfer with stromal vascular fraction to correct contour deformities of the autologous reconstructed breast. Aesthetic Plast Surg 45:853–863PubMedCrossRef

68.

Prantl L, Brix E, Kempa S, Felthaus O, Eigenberger A, Brébant V, Anker A, Strauss C (2021) Facial rejuvenation with concentrated lipograft: a 12 month follow-up study. Cells 10(3):594. https://doi.org/10.3390/cells10030594CrossRefPubMedPubMedCentral

69.

Zhu H, Quan Y, Wang J, Jiang SL, Lu F, Cai J, Liao Y (2021) Improving low-density fat by condensing cellular and collagen content through a mechanical process: basic research and Clinical applications. Plast Reconstr Surg. https://doi.org/10.1097/PRS.0000000000008484CrossRefPubMed

70.

Kølle SF, Duscher D, Taudorf M, Fischer-Nielsen A, Svalgaard JD, Munthe-Fog L, Jønsson B, Selvig PB, Mamsen FP, Katz AJ (2020) Ex vivo-expanded autologous adipose tissue-derived stromal cells ensure enhanced fat graft retention in breast augmentation: a randomized controlled clinical trial. Stem Cells Transl Med 9(11):1277–1286PubMedPubMedCentralCrossRef

71.

Gentile P, Sterodimas A, Calabrese C, De Angelis B, Trivisonno A, Pizzicannella J, Dionisi L, De Fazio D, Garcovich S (2020) Regenerative application of stromal vascular fraction cells enhanced fat graft maintenance: clinical assessment in face rejuvenation. Expert Opinion Biol Ther 20(12):1503–1513CrossRef

72.

Jung HK, Kim CH, Song SY (2016) Prospective 1-year follow-up study of breast augmentation by cell-assisted lipotransfer. Aesthet Surg J 36:179–190PubMedCrossRef

73.

Sasaki GH (2015) The safety and efficacy of cell-assisted fat grafting to traditional fat grafting in the anterior mid-face: an indirect assessment by 3D imaging. Aesthetic Plast Surg 39:833–846PubMedCrossRef

74.

Wang L, Luo X, Lu Y, Fan ZH, Hu X (2015) Is the resorption of grafted fat reduced in cell-assisted lipotransfer for breast augmentation? Ann Plast Surg 75(2):128–34PubMedCrossRef

75.

Kølle SFT, Fischer-Nielsen A, Mathiasen AB, Elberg JJ, Oliveri RS, Glovinski PV, Kastrup J, Kirchhoff M, Rasmussen BS, Talman MLM, Thomsen C, Dickmeiss E, Drzewiecki KT (2013) Enrichment of autologous fat grafts with ex-vivo expanded adipose tissue-derived stem cells for graft survival: a randomised placebo-controlled trial. Lancet 382(9898):1113–1120. https://doi.org/10.1016/S0140-6736(13)61410-5CrossRefPubMed

76.

Keyhan SO, Hemmat S, Badri AA, Abdeshahzadeh A, Khiabani K (2013) Use of platelet-rich fibrin and platelet-rich plasma in combination with fat graft: which is more effective during facial lipostructure? J Oral Maxillofacial Surg 71(3):610–621. https://doi.org/10.1016/j.joms.2012.06.176CrossRef

77.

Geeroms M, Fujimura S, Aiba E, Orgun D, Arita K, Kitamura R, Senda D, Mizuno H, Hamdi M, Tanaka R (2021) Quality and quantity-cultured human mononuclear cells improve human fat graft vascularization and survival in an in vivo murine experimental model. Plast Reconstr Surg 147:373–385PubMedCrossRef

78.

Rasmussen BS, Sørensen CL, Kurbegovic S, Ørholt M, Talman M-LM, Herly M, Pipper CB, Kølle S-FT, Rangatchew F, Holmgaard R, Vester-Glowinski PV, Fischer-Nielsen A, Drzewiecki KT (2019) Cell-enriched fat grafting improves graft retention in a porcine model: a dose-response study of adipose-derived stem cells versus stromal vascular fraction. Plast Reconstr Surg 144:397e–408ePubMedCrossRef

79.

Harris WM, Plastini M, Kappy N, Ortiz T, Chang S, Brown S, Carpenter JP, Zhang P (2019) Endothelial differentiated adipose-derived stem cells improvement of survival and neovascularization in fat transplantation. Aesthet Surg J 39:220–232PubMedCrossRef

80.

Choi JW, Kim SC, Eun-Jung Park JA (2018) Positive effect of incubated adipose-derived mesenchymal stem cells on microfat graft survival. J Craniofacial Surg 29(1):243–247. https://doi.org/10.1097/SCS.0000000000004071CrossRef

81.

Cai J, Feng J, Liu K, Zhou S, Lu F (2018) Early macrophage infiltration improves fat graft survival by inducing angiogenesis and hematopoietic stem cell recruitment. Plast Reconstr Surg 141:376–386PubMedCrossRef

82.

Zhang Y, Cai J, Zhou T, Yao Y, Dong Z, Lu F (2018) Improved long-term volume retention of stromal vascular fraction gel grafting with enhanced angiogenesis and adipogenesis. Plast Reconstr Surg 141:676e–686ePubMedCrossRef

83.

Zielins ER, Brett EA, Blackshear CP, Flacco J, Ransom RC, Longaker MT, Wan DC (2017) Purified adipose-derived stromal cells provide superior fat graft retention compared with unenriched stromal vascular fraction. Plast Reconstr Surg 139:911–914PubMedPubMedCentralCrossRef

84.

Kakudo N, Morimoto N, Ogawa T, Hihara M, Lai F, Kusumoto K (2017) Adipose-derived stem cell (ASC)-enriched fat grafting: experiments using White rabbits and an automated cell processing apparatus. Med Mol Morphol 50(3):170–177PubMedCrossRef

85.

Li K, Li F, Li J, Wang H, Zheng X, Long J, Guo W, Tian W (2017) Increased survival of human free fat grafts with varying densities of human adipose-derived stem cells and platelet-rich plasma. J Tissue Eng Regen Med 11:209–219PubMedCrossRef

86.

Li F, Guo W, Li K, Mei Y, Tang W, Wang H, Tian W (2015) Improved fat graft survival by different volume fractions of platelet-rich plasma and adipose-derived stem cells. Aesthetic Surg J 35(3):319–333. https://doi.org/10.1093/asj/sju046CrossRef

87.

Phipps KD, Gebremeskel S, Gillis J, Hong P, Johnston B, Bezuhly M (2015) Alternatively Activated M2 macrophages improve autologous fat graft survival in a mouse model through induction of angiogenesis. Plast Reconstr Surg 135:140–149PubMedCrossRef

88.

Paik KJ, Zielins ER, Atashroo DA, Maan ZN, Duscher D, Luan A, Walmsley GG, Momeni A, Vistnes S, Gurtner GC, Longaker MT, Wan DC (2015) Studies in fat grafting: part V. cell-assisted lipotransfer to enhance fat graft retention is dose dependent. Plast Reconstr Surg 136:67–75PubMedPubMedCentralCrossRef

89.

Bae YC, Song JS, Bae SH, Kim JH (2015) Effects of human adipose-derived stem cells and stromal vascular fraction on cryopreserved fat transfer. Dermatol Surg 41(5):605–614. https://doi.org/10.1097/DSS.0000000000000342CrossRefPubMed

90.

Aronowitz JA, Lockhart RA, Hakakian CS, Hicok KC (2015) Clinical safety of stromal vascular fraction separation at the point of care. Ann Plast Surg 75:666–671PubMedCrossRef

91.

Bae YC, Kim KH, Yun HJ, Oh CH, Chang JH, Yi CR, Lee JW, Bae SH (2020) A study on the effective ratio of fat to stromal vascular fraction for cell-assisted lipotransfer. Aesthetic Plast Surg 44:162–167PubMedCrossRef

92.

Li FW, Wang HB, Fang JP, Zeng L, Chen CL, Luo SK (2019) Optimal use ratio of the stromal vascular fraction (SVF): an animal experiment based on micro-ct dynamic detection after large-volume fat grafting. Aesthet Surg J 39(6):NP213–NP24PubMedCrossRef

93.

Yao Y, Dong Z, Liao Y, Zhang P, Ma J, Gao J, Lu F (2017) Adipose extracellular matrix/stromal vascular fraction gel: a novel adipose tissue-derived injectable for stem cell therapy. Plast Reconstr Surg 139:867–879PubMedCrossRef

94.

Prantl L, Eigenberger A, Klein S, Limm K, Oefner PJ, Schratzenstaller T, Felthaus O (2020) Shear force processing of lipoaspirates for stem cell enrichment does not affect secretome of human cells detected by mass spectrometry in vitro. Plast Reconstr Surg 146:749e–758ePubMedCrossRef

95.

Sun M, He Y, Zhou T, Zhang P, Gao J, Lu F (2017) Adipose extracellular matrix/stromal vascular fraction gel secretes angiogenic factors and enhances skin wound healing in a murine model. Biomed Res Int 2017:3105780PubMedPubMedCentralCrossRef

96.

Zhu H, Ge J, Chen X, Feng L, Cai J (2019) Mechanical micronization of lipoaspirates for regenerative therapy. J Vis Exp. https://doi.org/10.3791/58765CrossRefPubMed

97.

Zhang P, Feng J, Liao Y, Cai J, Zhou T, Sun M, Gao J, Gao K (2018) Ischemic flap survival improvement by composition-selective fat grafting with novel adipose tissue derived product-stromal vascular fraction gel. Biochem Biophys Res Commun 495(3):2249–2256PubMedCrossRef

98.

Yao Y, Cai J, Zhang P, Liao Y, Yuan Y, Dong Z, Lu F (2018) Adipose stromal vascular fraction gel grafting: a new method for tissue volumization and rejuvenation. Dermatol Surg 44:1278–1286PubMedCrossRef

99.

van Joris A, Dongen AJ, Tuin MS, Jansma J, van der Lei B, Harmsen MC (2018) Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review: intraoperative procedures for stromal vascular fraction isolation. J Tissue Eng Regener Med 12(1):e261–e274. https://doi.org/10.1002/term.2407CrossRef

100.

Vilaboa SDA, Navarro-Palou M, Llull R (2014) Age influence on stromal vascular fraction cell yield obtained from human lipoaspirates. Cytotherapy 16(8):1092–1097. https://doi.org/10.1016/j.jcyt.2014.02.007CrossRef

101.

Philips BJ, Grahovac TL, Valentin JE, Chung CW, Bliley JM, Pfeifer ME, Roy SB, Dreifuss S, Kelmendi-Doko A, Kling RE, Ravuri SK, Marra KG, Donnenberg VS, Donnenberg AD, Rubin JP (2013) Prevalence of endogenous CD34+ adipose stem cells predicts human fat graft retention in a xenograft model. Plast Reconstr Surg 132:845–858PubMedPubMedCentralCrossRef

102.

Mazur S, Zołocińska A, Siennicka K, Janik-Kosacka K, Chrapusta A, Pojda Z (2018) Safety of adipose-derived cell (stromal vascular fraction–SVF) augmentation for surgical breast reconstruction in cancer patients. Adv Clin Exp Med 27(8):1085–1090PubMedCrossRef

103.

Borrelli MR, Patel RA, Blackshear C, Vistnes S, Diaz NM, Deleon SA, Shen AH, Sokol J, Momeni A, Nguyen D, Longaker MT, Wan DC (2020) CD34+CD146+ adipose-derived stromal cells enhance engraftment of transplanted fat. Stem Cells Transl Med 9(11):1389–1400. https://doi.org/10.1002/sctm.19-0195CrossRefPubMedPubMedCentral

104.

Deleon NM, Adem S, Lavin CV, Abbas DB, Griffin M, King ME, Borrelli MR, Patel RA, Fahy EJ, Lee D, Shen AH (2021) Angiogenic CD34+ CD146+ adipose-derived stromal cells augment recovery of soft tissue after radiotherapy. J Tissue Eng Regener Med 15(12):1105–1117CrossRef

105.

Lauvrud AT, Kelk P, Wiberg M, Kingham PJ (2017) Characterization of human adipose tissue-derived stem cells with enhanced angiogenic and adipogenic properties. J Tissue Eng Regener Med 11(9):2490–2502CrossRef

106.

Peer LA (1956) The neglected free fat graft. Plast Reconstr Surg 18(4):233–250. https://doi.org/10.1097/00006534-195610000-00001CrossRef

107.

Su F, Luan J, Xin M, Wang Q, Xiao R, Gao Y (2013) Fate of adipose-derived stromal vascular fraction cells after co-implantation with fat grafts: evidence of cell survival and differentiation in ischemic adipose tissue. Plast Reconstr Surg 132(2):363–373. https://doi.org/10.1097/PRS.0b013e31829588b3CrossRef

108.

Suga H, Eto H, Aoi N, Kato H, Araki J, Doi K, Higashino T, Yoshimura K (2010) Adipose tissue remodeling under ischemia: death of adipocytes and activation of stem/progenitor cells. Plast Reconstr Surg 126(6):1911–1923. https://doi.org/10.1097/PRS.0b013e3181f4468bCrossRefPubMed

109.

Mashiko T, Yoshimura K (2015) How does fat survive and remodel after grafting? Clin Plast Surg 42(2):181–190. https://doi.org/10.1016/j.cps.2014.12.008CrossRefPubMed

110.

Yoshimura K, Eto H, Kato H, Doi K, Aoi N (2011) In vivo manipulation of stem cells for adipose tissue repair/reconstruction. Regener Med 6(6s):33–41. https://doi.org/10.2217/rme.11.62CrossRef

111.

Zhu Y-Z, Zhang J, Hu X, Wang Z, Wu S, Yi Y (2020) Supplementation with extracellular vesicles derived from adipose-derived stem cells increases fat graft survival and browning in mice: a cell-free approach to construct beige fat from white fat grafting. Plast Reconstr Surg 145(8):1183–1195PubMedCrossRef

112.

Shapouri-Moghaddam A, Mohammadian S, Vazini H, Taghadosi M, Esmaeili SA, Mardani F, Seifi B, Mohammadi A, Afshari JT, Sahebkar A (2018) Macrophage plasticity, polarization, and function in health and disease. J Cell Physiol 233(9):6425–40PubMedCrossRef

113.

Gorgun C, Ceresa D, Lesage R, Villa F, Reverberi D, Balbi C, Santamaria S, Cortese K, Malatesta P, Geris L, Quarto R, Tasso R (2021) Dissecting the effects of preconditioning with inflammatory cytokines and hypoxia on the angiogenic potential of mesenchymal stromal cell (MSC)-derived soluble proteins and extracellular vesicles (EVs). Biomaterials 269:120633PubMedCrossRef

114.

Shang Q, Bai Y, Wang G, Song Q, Guo C, Zhang L, Wang Q (2015) Delivery of adipose-derived stem cells attenuates adipose tissue inflammation and insulin resistance in obese mice Through remodeling macrophage phenotypes. Stem Cells Dev 24:2052–2064PubMedCrossRef

115.

Hanson SE, Kim J, Hematti P (2013) Comparative analysis of adipose-derived mesenchymal stem cells isolated from abdominal and breast tissue. Aesthetic Surg J 33(6):888–898. https://doi.org/10.1177/1090820X13496115CrossRef

116.

Raposo G, Stoorvogel W (2013) Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol 200(4):373–383. https://doi.org/10.1083/jcb.201211138CrossRefPubMedPubMedCentral

117.

Lo Sicco C, Reverberi D, Balbi C, Ulivi V, Principi E, Pascucci L, Becherini P, Bosco MC, Varesio L, Franzin C, Pozzobon M, Cancedda R, Tasso R (2017) Mesenchymal stem cell-derived extracellular vesicles as mediators of anti-inflammatory effects: endorsement of macrophage polarization. Stem Cells Transl Med 6:1018–1028PubMedPubMedCentralCrossRef

118.

Zhao H, Shang Q, Pan Z, Bai Y, Li Z, Zhang H, Zhang Q, Guo C, Zhang L, Wang Q (2018) Exosomes from adipose-derived stem cells attenuate adipose inflammation and obesity through polarizing m2 macrophages and beiging in white adipose tissue. Diabetes 67:235–247PubMedCrossRef

119.

Vasquez-Dunddel D, Pan F, Zeng Q, Gorbounov M, Albesiano E, Fu J, Blosser RL, Tam AJ, Bruno T, Zhang H, Pardoll D (2013) STAT3 regulates arginase-I in myeloid-derived suppressor cells from cancer patients. J Clin Inv 123(4):1580–9CrossRef

120.

Monticelli LA, Buck MD, Flamar AL, Saenz SA, Tait ED, Wojno NA, Yudanin LC, Osborne MR, Hepworth SV, Tran HRR, Shah H, Cross JR, Diamond JM, Cantu E, Christie JD, Pearce EL, Artis D (2016) Arginase 1 is an innate lymphoid-cell-intrinsic metabolic checkpoint controlling type 2 inflammation. Nat Immunol 17(6):656–665. https://doi.org/10.1038/ni.3421CrossRefPubMedPubMedCentral

121.

Zhu M, Xue J, Lu S, Yuan Y, Liao Y, Qiu J, Liu C, Liao Q (2019) Anti-inflammatory effect of stromal vascular fraction cells in fat transplantation. Exp Therap Med 17(2):1435–9

122.

Kim H, Lee BK (2020) Anti-inflammatory effect of adipose-derived stromal vascular fraction on osteoarthritic temporomandibular joint synoviocytes. Tissue Eng Regen Med 17:351–362PubMedPubMedCentralCrossRef

123.

Bai Y, Yan XL, Ren J, Zeng Q, Li XD, Pei XT, Han Y (2018) Co-transplantation of exosomes derived from hypoxia-preconditioned adipose mesenchymal stem cells promotes neovascularization and graft survival in fat grafting. Biochem Biophys Res Commun 497(1):305–312PubMedCrossRef

124.

Sacilotto N, Chouliaras KM, Nikitenko LL, Lu YW, Fritzsche M, Wallace MD, Nornes S, García-Moreno F, Payne S, Bridges E, Liu K (2016) MEF2 transcription factors are key regulators of sprouting angiogenesis. Genes Dev 30(20):2297–2309PubMedPubMedCentralCrossRef

125.

Huang B, Huang LF, Zhao L, Zeng Z, Wang X, Cao D, Yang L, Ye Z, Chen X, Liu B, He TC (2020) Microvesicles (MIVs) secreted from adipose-derived stem cells (ADSCs) contain multiple microRNAs and promote the migration and invasion of endothelial cells. Genes Dis 7(2):225–234PubMedCrossRef

126.

Kang T, Jones TM, Naddell C, Bacanamwo M, Calvert JW, Thompson WE, Bond VC, Chen YE, Liu D (2016) Adipose-derived stem cells induce angiogenesis via microvesicle transport of miRNA-31. Stem Cells Transl Med 5(4):440–450PubMedPubMedCentralCrossRef

127.

Liang X, Zhang L, Wang S, Han Q, Zhao RC (2016) Exosomes secreted by mesenchymal stem cells promote endothelial cell angiogenesis by transferring miR-125a. J Cell Sci 129(11):2182–2189. https://doi.org/10.1242/jcs.170373CrossRefPubMed

128.

Bridge G, Monteiro R, Henderson S, Emuss V, Lagos D, Georgopoulou D, Patient R, Boshoff C (2012) The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis. Blood J Am Soc Hematol 120(25):5063–5072

129.

Zhu Y, Zhang J, Hu X, Wang Z, Wu S, Yi Y (2020) Extracellular vesicles derived from human adipose-derived stem cells promote the exogenous angiogenesis of fat grafts via the let-7/AGO1/VEGF signalling pathway. Sci Rep 10(1):1–4

130.

Lai RC, Yeo RW, Lim SK (2015) Mesenchymal stem cell exosomes. Semin Cell Dev Biol 40:82–88PubMedCrossRef

131.

Serocki M, Bartoszewska S, Janaszak-Jasiecka A, Ochocka RJ, Collawn JF, Bartoszewski R (2018) miRNAs regulate the HIF switch during hypoxia: a novel therapeutic target. Angiogenesis 21(2):183–202. https://doi.org/10.1007/s10456-018-9600-2CrossRefPubMedPubMedCentral

132.

Suga H, Glotzbach JP, Sorkin M, Longaker MT, Gurtner GC (2014) Paracrine mechanism of angiogenesis in adipose-derived stem cell transplantation. Ann Plast Surg 72(2):234–241. https://doi.org/10.1097/SAP.0b013e318264fd6aCrossRefPubMedPubMedCentral

133.

Hao X, Guo Y, Wang R, Xueyuan Y, He L, Shu M (2021) Exosomes from adipose-derived mesenchymal stem cells promote survival of fat grafts by regulating macrophage polarization via let-7c. Acta Biochim et Biophys Sinica 53(4):501–510. https://doi.org/10.1093/abbs/gmab018CrossRef

134.

Nakamura Y, Miyaki S, Ishitobi H, Matsuyama S, Nakasa T, Kamei N, Akimoto T, Higashi Y, Ochi M (2015) Mesenchymal-stem-cell-derived exosomes accelerate skeletal muscle regeneration. FEBS Lett 589(11):1257–1265. https://doi.org/10.1016/j.febslet.2015.03.031CrossRefPubMed

135.

Merino-González C, Zuñiga FA, Escudero C, Ormazabal V, Reyes C, Nova-Lamperti E, Salomón C, Aguayo C (2016) Mesenchymal stem cell-derived extracellular vesicles promote angiogenesis: potencial clinical application. Front Physiol 7:24PubMedPubMedCentralCrossRef

136.

Lee JK, Park SR, Jung BK, Jeon YK, Lee YS, Kim MK, Kim YG, Jang JY, Kim CW (2013) Exosomes derived from mesenchymal stem cells suppress angiogenesis by down-regulating VEGF expression in breast cancer cells. PLoS ONE 8(12):e84256. https://doi.org/10.1371/journal.pone.0084256CrossRefPubMedPubMedCentral

137.

Qorri B, Kalaydina RV, Velickovic A, Kaplya Y, Decarlo A, Szewczuk MR (2018) Agonist-biased signaling via matrix metalloproteinase-9 promotes extracellular matrix remodeling. Cells 7(9):117PubMedPubMedCentralCrossRef

138.

Wu W, Peng S, Shi Y, Li L, Song Z, Lin S (2021) NPY promotes macrophage migration by upregulating matrix metalloproteinase-8 expression. J Cell Physiol 236(3):1903–1912PubMedCrossRef

139.

Chen B, Cai J, Wei Y, Jiang Z, Desjardins HE, Adams AE, Li S, Kao H-K, Guo L (2019) Exosomes are comparable to source adipose stem cells in fat graft retention with up-regulating early inflammation and angiogenesis. Plast Reconstr Surg 144:816e–827ePubMedCrossRef

140.

Mou S, Zhou M, Li Y, Wang J, Yuan Q, Xiao P, Sun J, Wang Z (2019) Extracellular vesicles from human adipose-derived stem cells for the improvement of angiogenesis and fat-grafting application. Plast Reconstr Surg 144:869–880PubMedCrossRef

141.

Billings E, May JW (1989) Historical review and present status of free fat graft autotransplantation in plastic and reconstructive surgery. Plast Reconstr Surg 83(2):368–831PubMedCrossRef

142.

Doi K, Ogata F, Eto H, Kato H, Kuno S, Kinoshita K, Kanayama K, Feng J, Manabe I, Yoshimura K (2015) Differential contributions of graft-derived and host-derived cells in tissue regeneration/remodeling after fat grafting. Plast Reconstr Surg 135:1607–1617PubMedCrossRef

143.

Kato H, Mineda K, Eto H, Doi K, Kuno S, Kinoshita K, Kanayama K, Yoshimura K (2014) Degeneration, regeneration, and cicatrization after fat grafting: dynamic total tissue remodeling during the first 3 months. Plast Reconstr Surg 133:303e–313ePubMedCrossRef

144.

Liao HT, James IB, Marra KG, Rubin JP (2015) The effects of platelet-rich plasma on cell proliferation and adipogenic potential of adipose-derived stem cells. Tissue Eng Part A 21:2714–2722PubMedPubMedCentralCrossRef

145.

Lai F, Kakudo N, Morimoto N, Taketani S, Hara T, Ogawa T, Kusumoto K (2018) Platelet-rich plasma enhances the proliferation of human adipose stem cells through multiple signaling pathways. Stem Cell Res Ther. https://doi.org/10.1186/s13287-018-0851-zCrossRefPubMedPubMedCentral

146.

Loibl M, Lang S, Hanke A, Herrmann M, Huber M, Brockhoff G, Klein S, Nerlich M, Angele P, Prantl L, Gehmert S (2016) Leukocyte-reduced platelet-rich plasma alters protein expression of adipose tissue-derived mesenchymal stem cells. Plast Reconstr Surg 138:397–408PubMedCrossRef

147.

Lopatina T, Bruno S, Tetta C, Kalinina N, Porta M, Camussi G (2014) Platelet-derived growth factor regulates the secretion of extracellular vesicles by adipose mesenchymal stem cells and enhances their angiogenic potential. Cell Commun Signal 12:26PubMedPubMedCentralCrossRef

148.

Mammoto T, Jiang A, Jiang E, Mammoto A (2013) Platelet rich plasma extract promotes angiogenesis through the angiopoietin1-Tie2 pathway. Microvasc Res 89:15–24. https://doi.org/10.1016/j.mvr.2013.04.008CrossRefPubMed

149.

Ghanaati S, Booms P, Orlowska A, Kubesch A, Lorenz J, Rutkowski J, Landes C, Robert Sader CJ, Kirkpatrick JC (2014) Advanced platelet-rich fibrin: a new concept for cell-based tissue engineering by means of inflammatory cells. J Oral Implantol 40(6):679–689. https://doi.org/10.1563/aaid-joi-D-14-00138CrossRefPubMed

150.

Masuki H, Okudera T, Watanebe T, Suzuki M, Nishiyama K, Okudera H, Nakata K, Uematsu K, Su CY, Kawase T (2016) Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int J Implant Dent 2:19PubMedPubMedCentralCrossRef

151.

Aizawa H, Tsujino T, Watanabe T, Isobe K, Kitamura Y, Sato A, Yamaguchi S, Okudera H, Okuda K, Kawase T (2020) Quantitative Near-infrared imaging of platelets in platelet-rich fibrin (PRF) matrices: comparative analysis of bio-prf, leukocyte-rich prf, advanced-prf and concentrated growth factors. Int J Mol Sci 21(12):4426PubMedPubMedCentralCrossRef

152.

Kobayashi E, Flückiger L, Fujioka-Kobayashi M, Sawada K, Sculean A, Schaller B, Miron RJ (2016) Comparative release of growth factors from PRP, PRF, and advanced-PRF. Clin Oral Inves 20(9):2353–2360CrossRef

153.

Calabriso N, Stanca E, Rochira A, Damiano F, Giannotti L, Di Chiara SB, Massaro M, Scoditti E, Demitri C, Nitti P, Palermo A (2021) Angiogenic properties of concentrated growth factors (CGFs): The role of soluble factors and cellular components. Pharmaceutics 13(5):635PubMedPubMedCentralCrossRef

154.

Del Vecchio DA, Del Vecchio SJ (2014) The graft-to-capacity ratio: volumetric planning in large-volume fat transplantation. Plast Reconstr Surg. 133:561–569PubMedCrossRef

155.

Tonnard P, Verpaele A, Peeters G, Hamdi M, Cornelissen M, Declercq H (2013) Nanofat grafting: basic research and clinical applications. Plast Reconstr Surg 132:1017–1026PubMedCrossRef

156.

Rose JG, Lucarelli MJ, Lemke BN, Dortzbach RK, Boxrud CA, Obagi S, Patel S (2006) Histologic comparison of autologous fat processing methods. Ophthalmic Plast Reconstr Surg 22(3):195–200PubMedCrossRef

157.

Bianchi F, Maioli M, Leonardi E, Olivi E, Pasquinelli G, Valente S, Mendez AJ, Ricordi C, Raffaini M, Tremolada C, Ventura C (2013) A new nonenzymatic method and device to obtain a fat tissue derivative highly enriched in pericyte-like elements by mild mechanical forces from human lipoaspirates. Cell Transpl 22:2063–2077CrossRef

158.

Zhu M, Dong Z, Gao J, Liao Y, Xue J, Yuan Y, Liu L, Chang Q, Lu F (2015) Adipocyte regeneration after free fat transplantation: promotion by stromal vascular fraction cells. Cell Transpl 24:49–62CrossRef

159.

Jung DW, Kim YH, Kim TG, Lee JH, Chung KJ, Lim JO, Choi JY (2015) Improvement of fat transplantation: fat graft with adipose-derived stem cells and oxygen-generating microspheres. Ann Plast Surg 75:463–470PubMedCrossRef

160.

Hong KY, Yim S, Kim HJ, Jin US, Lim S, Eo S, Chang H, Minn KW (2018) The fate of the adipose-derived stromal cells during angiogenesis and adipogenesis after cell-assisted lipotransfer. Plast Reconstr Surg 141:365–375PubMedCrossRef

161.

Guillaume VGJ, Ruhl T, Boos AM, Beier JP (2022) The crosstalk between adipose-derived stem or stromal cells (ASC) and cancer cells and asc-mediated effects on cancer formation and progression—ASCs: safety hazard or harmless source of tropism? Stem Cells Transl Med 11(4):394–406. https://doi.org/10.1093/stcltm/szac002CrossRefPubMedPubMedCentral

162.

Almeria C, Weiss R, Roy M, Tripisciano C, Kasper C, Weber V, Egger D (2019) Hypoxia conditioned mesenchymal stem cell-derived extracellular vesicles induce increased vascular tube formation in vitro. Front Bioeng Biotechnol. https://doi.org/10.3389/fbioe.2019.00292CrossRefPubMedPubMedCentral

163.

Seyhan N, Alhan D, Ural AU, Gunal A, Avunduk MC, Savaci N (2015) The effect of combined use of platelet-rich plasma and adipose-derived stem cells on fat graft survival. Ann Plast Surg 74(5):615–620. https://doi.org/10.1097/SAP.0000000000000480CrossRefPubMed

164.

Liang Z, Huang D, Nong W, Mo J, Zhu D, Wang M, Chen M, Wei C, Li H (2021) Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro. Tissue Cell 71:101506. https://doi.org/10.1016/j.tice.2021.101506CrossRefPubMed

165.

An Y, Panayi AC, Mi B, Fu S, Orgill DP (2020) Comparative analysis of two automated fat-processing systems. Plast Reconstr Surg Glob Open 8:e2587PubMedPubMedCentralCrossRef

Titel: Strategies to Improve AFT Volume Retention After Fat Grafting
verfasst von: Meiling Liu
Yujia Shang
Na Liu
Yonghuan Zhen
Youbai Chen
Yang An
Publikationsdatum: 31.10.2022
Verlag: Springer US
Erschienen in: Aesthetic Plastic Surgery / Ausgabe 2/2023
Print ISSN: 0364-216X
Elektronische ISSN: 1432-5241
DOI: https://doi.org/10.1007/s00266-022-03088-y

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Strategies to Improve AFT Volume Retention After Fat Grafting

Abstract

Background

Aim

Method

Results

Conclusion

Level of Evidence IV

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Abstract

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Method

Results

Conclusion

Level of Evidence IV

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