Erschienen in:
01.03.2016 | Original Paper
Presymptomatic activation of the PDGF-CC pathway accelerates onset of ALS neurodegeneration
verfasst von:
Sebastian A. Lewandowski, Ingrid Nilsson, Linda Fredriksson, Peter Lönnerberg, Lars Muhl, Manuel Zeitelhofer, Milena Z. Adzemovic, Susanne Nichterwitz, Daniel A. Lawrence, Eva Hedlund, Ulf Eriksson
Erschienen in:
Acta Neuropathologica
|
Ausgabe 3/2016
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Abstract
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease with unknown origins. Neurodegeneration in ALS mouse models occurs together with signs of disrupted blood–spinal cord barrier (BSCB) and regressed capillary network, but the molecular pathways contributing to these vascular pathologies remain unknown. We show that motor neurons of human sporadic ALS patients (n = 12) have increased gene expression of PDGFC and its activator PLAT and presymptomatic activation of the PDGF-CC pathway in SOD1
G93A
mice leads to BSCB dysfunction. Decrease of Pdgfc expression in SOD1
G93A
mice restored vascular barrier properties, reduced motor neuron loss and delayed symptom onset by up to 3 weeks. Similarly, lower expression levels of PDGFC or PLAT in motor neurons of sporadic ALS patients were correlated with older age at disease onset. PDGF-CC inhibition and restoration of BSCB integrity did not prevent capillary regression at disease end stage. Lower vessel density was found in spinal cords of sporadic ALS patients and the degree of regression in SOD1
G93A
mice correlated with more aggressive progression after onset regardless of BSCB status. We conclude that PDGF-CC-induced BSCB dysfunction can contribute to timing of ALS onset, allow insight into disease origins and development of targeted novel therapies.