nach oben

Erschienen in:

01.03.2004 | Original Article

Acute and severe hypobaric hypoxia-induced muscle oxidative stress in mice: the role of glutathione against oxidative damage

verfasst von: José Magalhães, António Ascensão, José M. C. Soares, Maria J. Neuparth, Rita Ferreira, José Oliveira, Francisco Amado, José A. Duarte

Erschienen in: European Journal of Applied Physiology | Ausgabe 2-3/2004

Einloggen, um Zugang zu erhalten

Abstract

This study intended to analyze: (1) the effects of acute and severe hypoxia exposure on skeletal muscle oxidative stress and oxidative damage markers; (2) the protective role of the antioxidant glutathione against oxidative damage; and (3) the expression of heat shock protein 70 kDa (HSP70) induced by this hypoxic insult. Forty mice were divided into four groups: control + placebo (C+P), hypoxia + placebo (H+P), control + l-buthionine-[S,R]-sulfoximine (BSO, a GSH-depleting compound) (C+BSO) and hypoxia + BSO (H+BSO). Hypoxia groups were continuously exposed for 24 h to a hypobaric hypoxic environment equivalent to an altitude of 7000 m and sacrificed immediately after. Control groups were maintained at sea level during the experimental protocol. Analyzed biochemical parameters were: reduced (GSH) and oxidized (GSSG) glutathione, thiobarbituric acid reactive substances (TBARS), sulfhydryl protein groups (SH), N-acetyl-β-d-glucosaminidase (NAG) and HSP70 protein. Hypoxia (H+P) per se, compared to C+P, induced a significant increase in %GSSG (5.68 vs. 1.14%), TBARS (436.7 vs. 227.9 nM), NAG (4.49 vs. 3.35 U/mg) and HSP70 (178.7 vs. 100%). Compared with H+P, H+BSO showed a significant decrease in total glutathione (19.30 vs. 6.13 nmol/mg) and an additional increase in %GSSG (5.68 vs. 11.33%) and in HSP70 expression (178.7 vs. 202.2%). However, no further oxidative damage was observed in H+BSO. These data suggest that acute hypoxia per se might enhance oxidative stress; however, the glutathione system seems to have a modest role in skeletal muscle protection against hypoxia-induced oxidative stress. Moreover, hypoxia and BSO treatment is a sufficient stimulus to promote HSP70 overexpression.

Askew EW (2002) Work at high altitude and oxidative stress: antioxidant nutrients. Toxicology 180:107–119CrossRefPubMed

Bailey D, Davies B, Davison G, Young I (2000) Oxidatively stressed out at high-altitude! Int Soc Mount Med Newsl 10:3–13

Bailey DM, Davies B, Young IS (2001) Intermittent hypoxic training: implications for lipid peroxidation induced by acute normoxic exercise in active men. Clin Sci (Lond) 101:465–475

Bertholf RL, Nicholson JR, Wills MR, Savory J (1987) Measurement of lipid peroxidation products in rabbit brain and organs (response to aluminum exposure). Ann Clin Lab Sci 17:418–423PubMed

Duranteau J, Chandel NS, Kulisz A, Shao Z, Schumacker PT (1998) Intracellular signaling by reactive oxygen species during hypoxia in cardiomyocytes. J Biol Chem 273:11619–11624PubMed

Feder ME, Hofmann GE (1999) Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology. Annu Rev Physiol 61:243–282PubMed

Griffith OW (1982) Mechanism of action, metabolism, and toxicity of buthionine sulfoximine and its higher homologs, potent inhibitors of glutathione synthesis. J Biol Chem 257:13704–13712PubMed

Griffith OW, Meister A (1979) Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butyl homocysteine sulfoximine). J Biol Chem 254:7558–7560PubMed

Halliwell B, Gutteridge JM (1999) Free radicals in biology and medicine. Oxford University Press, New York

Hamilton K, Powers S (2002) Heat shock proteins and reactive oxygen species. In: Noble E (ed) Exercise and stress response – the role of stress proteins. CRC, Boca Raton, Fla., pp 123–135

Hu M-L (1990) Measurement of protein thiol groups and GSH in plasma. In: Parker L (ed) Methods in Enzymology. Academic Press, San Diego, pp 380–385

Ji LL (1999) Antioxidants and oxidative stress in exercise. Proc Soc Exp Biol Med 222:283–292PubMed

Ji LL, Leeuwenburgh C (1996) Glutathione and exercise. In: Somani S (ed) Pharmacology in exercise and sports. CRC, Boca Raton, Fla., pp 97–123

Ji LL, Fu RG, Mitchell EW, Griffiths M, Waldrop TG, Swartz HM (1994) Cardiac hypertrophy alters myocardial response to ischaemia and reperfusion in vivo. Acta Physiol Scand 151:279–290PubMed

Joanny P, Steinberg J, Robach P, Richalet JP, Gortan C, Gardette B, Jammes Y (2001) Operation Everest III (Comex’97): the effect of simulated sever hypobaric hypoxia on lipid peroxidation and antioxidant defence systems in human blood at rest and after maximal exercise. Resuscitation 49:307–314CrossRefPubMed

Kehrer JP, Lund LG (1994) Cellular reducing equivalents and oxidative stress. Free Radic Biol Med 17:65–75PubMed

Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685PubMed

Leeuwenburgh C, Ji LL (1995) Glutathione depletion in rested and exercised mice: biochemical consequence and adaptation. Arch Biochem Biophys 316:941–949CrossRefPubMed

Leichtweis S, Ji LL (2001) Glutathione deficiency intensifies ischaemia-reperfusion induced cardiac dysfunction and oxidative stress. Acta Physiol Scand 172:1–10CrossRefPubMed

Locke M, Noble EG, Atkinson BG (1990) Exercising mammals synthesize stress proteins. Am J Physiol 258:C723–C729PubMed

Locke M, Noble EG, Tanguay RM, Feild MR, Ianuzzo SE, Ianuzzo CD (1995) Activation of heat-shock transcription factor in rat heart after heat shock and exercise. Am J Physiol 268:C1387–C1394PubMed

Lowry OH, Rosenbrough N, Farr AL, Radall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

Mohamed HE, El-Swefy SE, Hagar HH (2000) The protective effect of glutathione administration on adriamycin-induced acute cardiac toxicity in rats. Pharmacol Res 42:115–121CrossRefPubMed

Mohanraj P, Merola AJ, Wright VP, Clanton TL (1998) Antioxidants protect rat diaphragmatic muscle function under hypoxic conditions. J Appl Physiol 84:1960–1966

Moller P, Loft S, Lundby C, Olsen NV (2001) Acute hypoxia and hypoxic exercise induce DNA strand breaks and oxidative DNA damage in humans. FASEB J 15:1181–1186CrossRefPubMed

Noble E (2002) Heat shock proteins and their induction with exercise. In: Noble E (ed) Exercise and stress response. The role of stress proteins. CRC, Boca Raton, Fla., pp 43–78

Powers SK, Ji LL, Leeuwenburgh C (1999) Exercise training-induced alterations in skeletal muscle antioxidant capacity: a brief review. Med Sci Sports Exerc 31:987–997PubMed

Radak Z, Asano K, Lee KC, Ohno H, Nakamura A, Nakamoto H, Goto S (1997) High altitude training increases reactive carbonyl derivatives but not lipid peroxidation in skeletal muscle of rats. Free Radic Biol Med 22:1109–1114PubMed

Risby TH, Jiang L, Stoll S, Ingram D, Spangler E, Heim J, Cutler R, Roth GS, Rifkind JM (1999) Breath ethane as a marker of reactive oxygen species during manipulation of diet and oxygen tension in rats. J Appl Physiol 86:617–622PubMed

Salminen A, Marjomaki V (1985) Phosphomannosyl receptors of lysosomal enzymes in cardiac and skeletal muscles of young and old mice. Comp Biochem Physiol B 82:259–262CrossRefPubMed

Samaja M (1997) Blood gas transport at high altitude. Respiration 64:422–428

Sarada SK, Dipti P, Anju B, Pauline T, Kain AK, Sairam M, Sharma SK, Ilavazhagan G, Kumar D, Selvamurthy W (2002a) Antioxidant effect of beta-carotene on hypoxia induced oxidative stress in male albino rats. J Ethnopharmacol 79:149–153CrossRefPubMed

Sarada SK, Sairam M, Dipti P, Anju B, Pauline T, Kain AK, Sharma SK, Bagawat S, Ilavazhagan G, Kumar D (2002b) Role of selenium in reducing hypoxia-induced oxidative stress: an in vivo study. Biomed Pharmacother 56:173–178CrossRefPubMed

Sen CK (2001) Update on thiol status and supplements in physical exercise. Can J Appl Physiol Suppl 26:S4–S12

Sen CK, Rahkila P, Hanninen O (1993) Glutathione metabolism in skeletal muscle derived cells of the L6 line. Acta Physiol Scand 148:21–26PubMed

Sen CK, Atalay M, Hanninen O (1994) Exercise-induced oxidative stress: glutathione supplementation and deficiency. J Appl Physiol 77:2177–2187PubMed

Simon-Schnass I (2000) Risk of oxidative stress during exercise at high altitude. In: Hannienen O (ed) Handbook of oxidants and antioxidants in exercise. Elsevier, Amsterdam, pp 191–210

Singh SN, Vats P, Kumria MM, Ranganathan S, Shyam R, Arora MP, Jain CL, Sridharan K (2001) Effect of high altitude (7,620 m) exposure on glutathione and related metabolism in rats. Eur J Appl Physiol 84:233–237

Thannickal VJ, Fanburg BL (2000) Reactive oxygen species in cell signaling. Am J Physiol 279:L1005–L1028

Tietze F (1969) Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem 27:502–522PubMed

Vasankari TJ, Kujala UM, Rusko H, Sarna S, Ahotupa M (1997) The effect of endurance exercise at moderate altitude on serum lipid peroxidation and antioxidative functions in humans. Eur J Appl Physiol 75:396–399CrossRef

Venditti P, Di Meo S (1996) Antioxidants, tissue damage, and endurance in trained and untrained young male rats. Arch Biochem Biophys 331:63–68CrossRefPubMed

Titel: Acute and severe hypobaric hypoxia-induced muscle oxidative stress in mice: the role of glutathione against oxidative damage
verfasst von: José Magalhães
António Ascensão
José M. C. Soares
Maria J. Neuparth
Rita Ferreira
José Oliveira
Francisco Amado
José A. Duarte
Publikationsdatum: 01.03.2004
Verlag: Springer-Verlag
Erschienen in: European Journal of Applied Physiology / Ausgabe 2-3/2004
Print ISSN: 1439-6319
Elektronische ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-003-0972-6

Neu im Fachgebiet Arbeitsmedizin

Das Geschlechterparadoxon in der gesundheitlich beeinträchtigten Lebenszeit – Ende eines Mythos?

Open Access Hüftgelenksbewegungseinschränkung Leitthema

Beginnend mit den 1920er-Jahren hat sich eine Vorstellung über die Geschlechterdifferenzen in Gesundheit und Mortalität etabliert, die von Lorber und Moore in dem einprägsamen Satz: „Women get sicker, but men die quicker“, zusammengefasst wurde [ 1 …, S. 13]. Tatsächlich erscheinen vor dem Hintergrund der höheren Lebenserwartung der Frauen die Studienergebnisse zu den Geschlechterdifferenzen in der Morbidität überraschend, wonach Frauen im Durchschnitt einen schlechteren Gesundheitszustand aufweisen als Männer [

Gesunde Lebenserwartung: Ein kritischer Blick auf Nutzen und Potenziale des demographischen Gesundheitsindikators

Open Access Leitthema

Die demographische Alterung hat vielfältige gesellschaftliche Konsequenzen, deren Ausmaß wesentlich vom Gesundheitszustand der Bevölkerung abhängt. Um diesen analysieren und bewerten zu können, wurden spezielle Kennziffern entwickelt, die in …

Wie hat sich die Lebenserwartung ohne funktionelle Einschränkungen in Deutschland entwickelt? Eine Analyse mit Daten des Deutschen Alterssurveys (DEAS)

Open Access Hüftgelenksbewegungseinschränkung Leitthema

Deutschland erfährt, wie andere Hocheinkommensstaaten, aufgrund kontinuierlich rückläufiger Mortalität und niedriger Geburtenraten tiefgreifende demografische Veränderungen. Der demografische Wandel führt in Deutschland zu einem zunehmend höheren …

Hitzeschutz im Fokus der hessischen Betreuungs- und Pflegeaufsicht

Open Access Klimawandel Übersichtsartikel

Im Sommer 2023 kündigte das Bundesministerium für Gesundheit (BMG) einen nationalen Hitzeschutzplan an und forderte die Länder auf, zu prüfen, „ob die Warnstufen des [Deutschen Wetterdienstes] DWD mit der Durchführung von Akutmaßnahmen …

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Acute and severe hypobaric hypoxia-induced muscle oxidative stress in mice: the role of glutathione against oxidative damage

Abstract

Neu im Fachgebiet Arbeitsmedizin

Das Geschlechterparadoxon in der gesundheitlich beeinträchtigten Lebenszeit – Ende eines Mythos?

Gesunde Lebenserwartung: Ein kritischer Blick auf Nutzen und Potenziale des demographischen Gesundheitsindikators

Wie hat sich die Lebenserwartung ohne funktionelle Einschränkungen in Deutschland entwickelt? Eine Analyse mit Daten des Deutschen Alterssurveys (DEAS)

Hitzeschutz im Fokus der hessischen Betreuungs- und Pflegeaufsicht

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2-3/2004

Validity of uniaxial accelerometry during activities of daily living in children

Knee and ankle range of motion during stepping down in elderly compared to young men

Effects of power training on mechanical efficiency in jumping

Simple method for the identification of oxidative fibers in skeletal muscle

Relationship between double product break point, lactate threshold, and ventilatory threshold in cardiac patients

The critical power model for intermittent exercise

Neu im Fachgebiet Arbeitsmedizin

Das Geschlechterparadoxon in der gesundheitlich beeinträchtigten Lebenszeit – Ende eines Mythos?

Gesunde Lebenserwartung: Ein kritischer Blick auf Nutzen und Potenziale des demographischen Gesundheitsindikators

Wie hat sich die Lebenserwartung ohne funktionelle Einschränkungen in Deutschland entwickelt? Eine Analyse mit Daten des Deutschen Alterssurveys (DEAS)

Hitzeschutz im Fokus der hessischen Betreuungs- und Pflegeaufsicht