nach oben

Medical Microbiology and Immunology

Erschienen in:

01.11.2011 | Original Investigation

Specific detection of antibodies to different flaviviruses using a new immune complex ELISA

verfasst von: Herbert Schmitz, Martin Gabriel, Petra Emmerich

Erschienen in: Medical Microbiology and Immunology | Ausgabe 4/2011

Einloggen, um Zugang zu erhalten

Abstract

Immune complex (IC) ELISAs for IgG antibodies to various virus antigens have turned out to be both highly specific and sensitive. During incubation of a labelled antigen with the serum samples, ICs are formed, which bind to microtiter plates coated with rheumatoid factor (RF) IgM. Here, we describe an improved coating of the solid-phase support comparing various Fc-receptor molecules. IC ELISAs were applied to detect human IgG antibodies to the highly virus-specific ED3 domain of West Nile- and tick-borne encephalitis virus envelopes. Compared with other Fc-receptor molecules like RF or C1q, FcγRIIA (CD32) turned out to bind the ICs composed of IgG antibodies and peroxidase-labelled ED3 antigens more efficiently. Due to low background reactions, sera could be tested at a dilution of 1:10. Moreover, using CD32 instead of RF coating, anti-flavivirus antibodies could be detected in various animal species

Sachers M, Emmerich P, Mohr H, Schmitz H (1985) Simple detection of antibodies to different viruses using rheumatoid factor and enzyme-labelled antigen (ELA). J Virol Methods 10(2):99–110PubMedCrossRef

Emmerich P, Thome-Bolduan C, Drosten C, Gunther S, Ban E, Sawinsky I, Schmitz H (2006) Reverse ELISA for IgG and IgM antibodies to detect Lassa virus infections in Africa. J Clin Virol 37(4):277–281PubMedCrossRef

Ludolfs D, Niedrig M, Paweska JT, Schmitz H (2007) Reverse ELISA for the detection of anti West Nile virus IgG antibodies in humans. Eur J Clin Microbiol Infect Dis 26(7):467–473PubMedCrossRef

Ludolfs D, Reinholz M, Schmitz H (2009) Highly specific detection of antibodies to Tick-borne encephalitis (TBE) virus in humans using a domain III antigen and a sensitive immune complex (IC) ELISA. J Clin Virol 45(2):125–128PubMedCrossRef

Cuzzubbo AJ, Vaughn DW, Nisalak A, Solomon T, Kalayanarooj S, Aaskov J, Dung NM, Devine PL (1999) Comparison of PanBio dengue duo enzyme-linked immunosorbent assay (ELISA) and MRL dengue fever virus immunoglobulin M capture ELISA for diagnosis of dengue virus infections in Southeast Asia. Clin Diagn Lab Immunol 6(5):705–712PubMed

Vazquez S, Hafner G, Ruiz D, Calzada N, Guzman MG (2007) Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections. J Clin Virol 39(3):194–198PubMedCrossRef

Jefferis R, Mageed RA (1989) The specificity and reactivity of rheumatoid factors with human IgG. Monogr Allergy 26:45–60PubMed

Duquerroy S, Stura EA, Bressanelli S, Fabiane SM, Vaney MC, Beale D, Hamon M, Casali P, Rey FA, Sutton BJ, Taussig MJ (2007) Crystal structure of a human autoimmune complex between IgM rheumatoid factor RF61 and IgG1 Fc reveals a novel epitope and evidence for affinity maturation. J Mol Biol 368(5):1321–1331PubMedCrossRef

Bohlson SS, Fraser DA, Tenner AJ (2007) Complement proteins C1q and MBL are pattern recognition molecules that signal immediate and long-term protective immune functions. Mol Immunol 44(1–3):33–43PubMedCrossRef

10.

Astier A, de la Salle H, de la Salle C, Bieber T, Esposito-Farese ME, Freund M, Cazenave JP, Fridman WH, Teillaud JL, Hanau D (1994) Human epidermal Langerhans cells secrete a soluble receptor for IgG (Fc gamma RII/CD32) that inhibits the binding of immune complexes to Fc gamma R+cells. J Immunol 152(1):201–212PubMed

11.

Ravetch JV, Bolland S (2001) IgG Fc receptors. Annu Rev Immunol 19:275–290PubMedCrossRef

12.

Nimmerjahn F, Ravetch JV (2006) Fcgamma receptors: old friends and new family members. Immunity 24(1):19–28PubMedCrossRef

13.

Throsby M, Geuijen C, Goudsmit J, Bakker AQ, Korimbocus J, Kramer RA, Clijsters-van der Horst M, de Jong M, Jongeneelen M, Thijsse S, Smit R, Visser TJ, Bijl N, Marissen WE, Loeb M, Kelvin DJ, Preiser W, ter Meulen J, de Kruif J (2006) Isolation and characterization of human monoclonal antibodies from individuals infected with West Nile Virus. J Virol 80(14):6982–6992PubMedCrossRef

14.

Vance BA, Huizinga TW, Wardwell K, Guyre PM (1993) Binding of monomeric human IgG defines an expression polymorphism of Fc gamma RIII on large granular lymphocyte/natural killer cells. J Immunol 151(11):6429–6439PubMed

15.

Teeling JL, Jansen-Hendriks T, Kuijpers TW, de Haas M, van de Winkel JG, Hack CE, Bleeker WK (2001) Therapeutic efficacy of intravenous immunoglobulin preparations depends on the immunoglobulin G dimers: studies in experimental immune thrombocytopenia. Blood 98(4):1095–1099PubMedCrossRef

16.

Kimura-Kuroda J, Yasui K (1986) Antigenic comparison of envelope protein E between Japanese encephalitis virus and some other flaviviruses using monoclonal antibodies. J Gen Virol 67(Pt 12):2663–2672PubMedCrossRef

17.

Heinz FX, Berger R, Tuma W, Kunz C (1983) A topological and functional model of epitopes on the structural glycoprotein of Tick-borne encephalitis virus defined by monoclonal antibodies. Virology 126(2):525–537PubMedCrossRef

18.

Bruhns P, Iannascoli B, England P, Mancardi DA, Fernandez N, Jorieux S, Daeron M (2009) Specificity and affinity of human Fcgamma receptors and their polymorphic variants for human IgG subclasses. Blood 113(16):3716–3725PubMedCrossRef

19.

Hack CE, Belmer AJ (1986) The IgG detected in the C1q solid-phase immune-complex assay is not always of immune-complex nature. Clin Immunol Immunopathol 38(1):120–128PubMedCrossRef

20.

Theofilopoulos AN, Balderas RS, Hang L, Dixon FJ (1983) Monoclonal IgM rheumatoid factors derived from arthritic MRL/Mp-lpr/lpr mice. J Exp Med 158(3):901–919PubMedCrossRef

21.

Anderson CL, Guyre PM, Whitin JC, Ryan DH, Looney RJ, Fanger MW (1986) Monoclonal antibodies to Fc receptors for IgG on human mononuclear phagocytes. Antibody characterization and induction of superoxide production in a monocyte cell line. J biol chem 261(27):12856–12864PubMed

22.

Sukupolvi-Petty S, Austin SK, Purtha WE, Oliphant T, Nybakken GE, Schlesinger JJ, Roehrig JT, Gromowski GD, Barrett AD, Fremont DH, Diamond MS (2007) Type- and subcomplex-specific neutralizing antibodies against domain III of dengue virus type 2 envelope protein recognize adjacent epitopes. J Virol 81(23):12816–12826PubMedCrossRef

Titel: Specific detection of antibodies to different flaviviruses using a new immune complex ELISA
verfasst von: Herbert Schmitz
Martin Gabriel
Petra Emmerich
Publikationsdatum: 01.11.2011
Verlag: Springer-Verlag
Erschienen in: Medical Microbiology and Immunology / Ausgabe 4/2011
Print ISSN: 0300-8584
Elektronische ISSN: 1432-1831
DOI: https://doi.org/10.1007/s00430-011-0195-0

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Specific detection of antibodies to different flaviviruses using a new immune complex ELISA

Abstract

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Ist Fasten vor Koronarinterventionen wirklich nötig?

Typ-2-Diabetes: Ernährungsunsicherheit vervierfacht Risiko für schwere Hypoglykämien

Mehr Brustkrebs, aber weniger andere gynäkologische Tumoren mit Levonorgestrel-IUS

GLP-1-Agonist Semaglutid wirkt kardio- und nephroprotektiv

Update Innere Medizin

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 4/2011

Immune response in symptomatic and asymptomatic neurocysticercosis

Trypanosoma cruzi antigen immunization induces a higher B cell survival in BALB/c mice, a susceptible strain, compared to C57BL/6 B lymphocytes, a resistant strain to cardiac autoimmunity

Seroprevalence study in forestry workers of a non-endemic region in eastern Germany reveals infections by Tula and Dobrava–Belgrade hantaviruses

HIV prevalence and route of transmission in Turkish immigrants living in North-Rhine Westphalia, Germany

The SnoB study: frequency of baseline raltegravir resistance mutations prevalence in different non-B subtypes

Treatment with IP-10 induces host-protective immune response by regulating the T regulatory cell functioning in Leishmania donovani-infected mice

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Ist Fasten vor Koronarinterventionen wirklich nötig?

Typ-2-Diabetes: Ernährungsunsicherheit vervierfacht Risiko für schwere Hypoglykämien

Mehr Brustkrebs, aber weniger andere gynäkologische Tumoren mit Levonorgestrel-IUS

GLP-1-Agonist Semaglutid wirkt kardio- und nephroprotektiv

Update Innere Medizin