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Journal of Cancer Research and Clinical Oncology

Erschienen in:

01.06.2010 | Original Paper

Differential expression of miRNA patterns in renal cell carcinoma and nontumorous tissues

verfasst von: Zhengjun Yi, Yurong Fu, Shushu Zhao, Xuguang Zhang, Chuanxiang Ma

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 6/2010

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Abstract

Introduction

MiRNAs are short single stranded RNAs that are associated with gene regulation at the transcriptional and translational level. Changes in their expression were found in a variety of human cancers. In the present study, we aimed to investigate expression profiles of miRNA (miRNA) in the clear cell subtype of kidney cancer and to develop further understanding of the molecular mechanisms involved in the pathogenesis of renal cell carcinoma.

Materials and methods

We analyzed the miRNA expression profiles in 30 pairs of renal cell carcinoma and adjacent nontumorous tissue (NT), using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences. Real-time quantitative PCR was performed to confirm the array results.

Results

The miRNA microarray chip analysis identified 86 miRNAs differentially expressed in renal cell carcinoma tissues and a total of 38 miRNAs exhibited higher expression in the renal cell carcinoma samples than that in the NT samples, while 48 miRNA demonstrated lower expression in the renal cell carcinoma samples than that in the NT samples.

Conclusion

Quantitative real-time PCR analysis confirmed microarray data. The report supports that many miRNA expressions were altered in renal carcinoma, whose expression profiling may provide a useful clue for the pathophysiology research. However, further longer-term researches are required to investigate the relationship between miRNA and renal carcinoma as well as their role in carcinogenesis.

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Titel: Differential expression of miRNA patterns in renal cell carcinoma and nontumorous tissues
verfasst von: Zhengjun Yi
Yurong Fu
Shushu Zhao
Xuguang Zhang
Chuanxiang Ma
Publikationsdatum: 01.06.2010
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 6/2010
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-009-0726-x

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Differential expression of miRNA patterns in renal cell carcinoma and nontumorous tissues

Abstract

Introduction

Materials and methods

Results

Conclusion

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