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Journal of Cancer Research and Clinical Oncology

Erschienen in:

20.03.2018 | Original Article – Cancer Research

Cartilage oligomeric matrix protein is a prognostic factor and biomarker of colon cancer and promotes cell proliferation by activating the Akt pathway

verfasst von: Ting-ting Liu, Xi-sheng Liu, Meng Zhang, Xue-ni Liu, Fu-xiang Zhu, Fang-ming Zhu, Si-wen Ouyang, Shan-bao Li, Chen-long Song, Hui-min Sun, Su Lu, Yu Zhang, Jun Lin, Hua-mei Tang, Zhi-hai Peng

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 6/2018

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Abstract

Purpose

Recent studies have determined that cartilage oligomeric matrix protein (COMP) plays a vital role in carcinogenesis. We sought to clarify the role of COMP in colon cancer.

Methods

We investigated gene expression data from The Cancer Genome Atlas (TCGA) dataset. Tissue microarrays (TMA) containing paired samples from 253 patients with colon cancer were subjected to immunostaining. COMP levels in serum of colon cancer patients and healthy donors were measured with ELISA. We established COMP-knockout cells using the CRISPR/Cas9 system and COMP-overexpressing cells using lentiviral vectors to detect the effects of COMP on colon cancer cells using Cell Counting Kit-8 (CCK8), colony formation, apoptosis detection kit, and tumorigenesis assays in nude mice.

Results

The analysis of TCGA dataset and the results of the TMA suggested that COMP expression levels were significantly higher in cancer tissues than in adjacent normal tissues. Moreover, high COMP expression was correlated with the poor outcome of colon cancer patients. COMP levels in the sera of preoperative patients with colon cancer were much higher than those in healthy donors and were significantly reduced after colectomy. Colon cancer cells without COMP were defective with respect to the ability to proliferate, colony formation, the ability to resist 5-Fluorouracil-induced apoptosis and the growth of xenograft tumors in mice. Contrasting results were observed in COMP overexpressed cells. COMP promoted colon cancer cell proliferation partially through the activation of PI3K/ Akt/ mTOR/ p70S6K pathway.

Conclusions

COMP may be a novel prognostic indicator and biomarker and also a potential therapeutic target for colon cancer.

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Titel: Cartilage oligomeric matrix protein is a prognostic factor and biomarker of colon cancer and promotes cell proliferation by activating the Akt pathway
verfasst von: Ting-ting Liu
Xi-sheng Liu
Meng Zhang
Xue-ni Liu
Fu-xiang Zhu
Fang-ming Zhu
Si-wen Ouyang
Shan-bao Li
Chen-long Song
Hui-min Sun
Su Lu
Yu Zhang
Jun Lin
Hua-mei Tang
Zhi-hai Peng
Publikationsdatum: 20.03.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 6/2018
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-018-2626-4

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Cartilage oligomeric matrix protein is a prognostic factor and biomarker of colon cancer and promotes cell proliferation by activating the Akt pathway