nach oben

Erschienen in:

01.01.2014 | Original Article

Resveratrol supplementation preserves long bone mass, microstructure, and strength in hindlimb-suspended old male rats

verfasst von: Stephanie M. Durbin, Janna R. Jackson, Michael J. Ryan, Joseph C. Gigliotti, Stephan E. Alway, Janet C. Tou

Erschienen in: Journal of Bone and Mineral Metabolism | Ausgabe 1/2014

Einloggen, um Zugang zu erhalten

Abstract

Resveratrol has gained popularity as an “anti-aging” compound due to its antioxidant and anti-inflammatory properties. Few studies have investigated the role of resveratrol supplementation in the prevention of age-related bone loss and skeletal disuse despite increased inactivity and age-related bone loss in the elderly. The objective of the study was to investigate the effect of resveratrol supplementation on disuse and age-related bone loss. Old (age 33 months) Fischer 344 × Brown Norway male rats were provided either trans-resveratrol (12.5 mg/kg bw/day) or deionized distilled water by oral gavage for 21 days. Rats were hindlimb-suspended (HLS) or kept ambulatory (AMB) for 14 days. Both femora and tibiae were collected. Bone mass was measured by dual-energy X-ray absorptiometry and bone microstructure was determined by micro-computed tomography. HLS of old male rats accelerated loss of bone mineral content, decreased trabecular bone volume per unit of total volume, and increased trabecular separation. Resveratrol supplementation ameliorated bone demineralization and loss of bone microarchitecture in HLS old male rats. The peak force measured by the three-point bending test was reduced (P = 0.007) in HLS/control compared to AMB/control rats. Resveratrol supplementation ameliorated HLS-induced loss of femur strength. Plasma osteocalcin and alkaline phosphatase was higher (P < 0.04) and C-reactive protein was lower (P = 0.04) in old male rats given resveratrol. The bone protective effects of resveratrol appeared to be mediated through increased osteoblast bone formation, possibly due to reduced inflammation. Based on the results, resveratrol supplementation appeared to provide a feasible dietary therapy for preserving the skeletal system during disuse and age-related bone loss.

Duque G, Troen BR (2008) Understanding the mechanisms of senile osteoporosis: new facts for a major geriatric syndrome. J Am Geriatr Soc 56:935–941PubMedCrossRef

Kanis JA, Johnell O, Oden A (2001) Ten year probabilities of osteoporotic fractures according to BMD and diagnostic thresholds. Osteoporos Int 12:989–995PubMedCrossRef

Inderjeeth CA, Foo AC, Lai MM, Glendenning P (2009) Efficacy and safety of pharmacological agents in managing osteoporosis in the old: review of the evidence. Bone 44:744–751PubMedCrossRef

Ebeling PR (1998) Osteoporosis in men. New insights into aetiology, pathogenesis, prevention, and management. Drugs Aging 13:421–434PubMedCrossRef

Vidavalur R, Otani H, Singal PK, Maulik N (2006) Significance of wine and resveratrol in cardiovascular disease: French paradox revisited. Exp Clin Cardiol 11:217–225PubMedCentralPubMed

Cottart C, Nivet-Antoine V, Laguillier-Morizot, Beaudeux J (2010) Resveratrol bioavailability and toxicity in humans. Mol Nutr Food Res 54:7–16PubMedCrossRef

Williams LD, Burdock GA, Edwards JA, Beck M, Bausch J (2009) Safety studies conducted on high-purity trans-resveratrol in experimental animals. Food Chem Toxicol 47:2170–2182PubMedCrossRef

Chachay VS, Kirkpatrick CM, Hickman IJ, Ferguson M, Prins JB, Martin JH (2011) Resveratrol – pills to replace a healthy diet? Br J Clin Pharmacol 72:27–38PubMedCrossRef

Dehority W, Halloran BP, Bikle DD, Curren T, Kostenuik PJ, Wronski TJ, Shen Y, Rabkin B, Bouraoui A, Morey-Holton E (1999) Bone and hormonal changes induced by skeletal unloading in the mature male rat. Am J Physiol 276:E62–E69PubMed

10.

Habold C, Momken I, Ouadi A, Bekaert V, Brasse D (2011) Effect of prior treatment with RES on density and structure of rat long bones under tail-suspension. J Bone Miner Metab 29:15–22PubMedCrossRef

11.

Momken I, Stevens L, Bergouignan A, Desplanches D, Rudwill F, Chery I, Zahariev A, Zahn S, Stein TP, Sebedio JL, Pujos-Guillot E, Falempin M, Simon C, Coxam V, Andrianjafiniony T, Gauquelin-Koch G, Picquet F, Blanc S (2011) Resveratrol prevents the wasting disorders of mechanical unloading by acting as a physical exercise mimetic in the rat. FASEB J 25:3646–3660PubMedCrossRef

12.

Zhang YB, Zhong ZM, Hou G, Jiang H, Chen JT (2011) Involvement of oxidative stress in age-related bone loss. J Surg Res 169:37–42CrossRef

13.

Yun AJ, Lee PY (2004) Maldaptation of the link between inflammation and bone turnover may be a key determinant of osteoporosis. Med Hypotheses 63:532–537PubMedCrossRef

14.

Bar-Shai M, Carmeli E, Ljubuncic P, Reznick AZ (2008) Exercise and immobilization in aging animals: the involvement of oxidative stress and NF-kappaB activation. Free Radic Biol Med 44:202–214PubMedCrossRef

15.

Smith BJ, Lucas EA, Turner RT, Evans GL, Lerner MR, Brackett DJ, Stoecker BJ, Arjmandi BH (2005) Vitamin E provides production for bone in mature hindlimb unloaded male rats. Calcif Tissue Int 76:272–279PubMedCrossRef

16.

Boissy P, Andersen TL, Abdallah BM, Kassem M, Plesner T, Delaissé JM (2005) Resveratrol inhibits myeloma cell growth, prevents osteoclast formation, and promotes osteoblast differentiation. Cancer Res 65:9943–9952PubMedCrossRef

17.

Pervaiz S, Holme AL (2009) Resveratrol: its biologic targets and functional activity. Antioxid Redox Signal 11:2851–2880PubMedCrossRef

18.

Institute of Laboratory Animal Resources Commission on Life Sciences (1995) Guide for the care and use of laboratory animals. National Academy Press, Washington, pp 21–55

19.

Morey-Holton E, Globus RK (2002) The hindlimb unloading rodent model: technical aspects. J Appl Physiol 92:1367–1377PubMed

20.

Yuan YV, Kitts DD (1992) Estimation of dietary calcium utilization in rats using a biomechanical functional test. Food Chem 44:1–47CrossRef

21.

Ortoft G, Oxlund H, Jorgensen PH, Andreassen TT (1992) Glucocorticoid treatment of food deprivation counteracts the simulation effect of growth hormone on rat cortical bone strength. Acta Paediatr 81:912–917PubMedCrossRef

22.

Banu J, Wang L, Kalu DN (2002) Age-related changes in bone mineral content and density in intact male F344 rats. Bone 30:125–130PubMedCrossRef

23.

Wang L, Banu J, McMahan CA, Kalu DN (2001) Male rodent model of age-related bone loss in men. Bone 29:141–148PubMedCrossRef

24.

Iida H, Fukuda S (2002) Age-related changes in bone mineral density, cross-sectional area and strength at different skeletal sites in male rats. J Vet Med Sci 64:29–34PubMedCrossRef

25.

Chen H, Zhou X, Emura, Shoumura S (2009) Site-specific bone loss in senescence-accelerated mouse (SAMP6): a murine model for senile osteoporosis. Exp Gerontol 44:792–798PubMedCrossRef

26.

Pearson KJ, Baur JA, Lewis KN, Peshkin L, Price NL et al (2008) Resveratrol delays age-related deterioration and mimics transcriptional aspects of dietary restriction without extending lifespan. Cell Metab 8:157–168PubMedCentralPubMedCrossRef

27.

Jee WS, Ma Y (1999) Animal models of immobilization osteopenia. Morphologie 83:25–34PubMed

28.

Perrien DS, Akel NS, Dupont-Versteegden EE, Skinner RA, Siegel ER, Suva LJ, Gaddy D (2007) Aging alters the skeletal response to disuse in the rat. Am J Physiol Regul Integr Comp Physiol 292:988–996CrossRef

29.

Juan ME, González-Pons E, Munuera T, Ballester J, Rodríguez-Gil JE, Planas JM (2005) Trans-RES, a natural antioxidant from grapes, increases sperm output in healthy rats. J Nutr 135:757–760PubMed

30.

Vico L, Collet P, Guignandon A, Lafage-Proust MH, Thomas T, Rehaillia M, Alexandre C (2000) Effects of long-term microgravity exposure on cancellous and cortical weight-bearing bones of cosmonauts. Lancet 355:1607–1611PubMedCrossRef

31.

Li XJ, Jee WS, Ke HZ, Mori S, Akamine T (1991) Age-related changes of cancellous and cortical bone histomorphometry in female Sprague-Dawley rats. Cells Mater (Suppl) 1:25–37

32.

Bloomfield SA, Allen MR, Hogan HA, Delp MD (2003) Site and compartment specific changes in bone with hindlimb unloading in mature adult rats. Bone 31:149–157CrossRef

33.

Hayes DP (2011) Resveratrol and vitamin D: significant potential interpretive problems arising from their mutual processes, interactions and effects. Med Hypotheses 77:765–772PubMedCrossRef

34.

Gropper SS, Smith JL, Groff JL (2009) The fat-soluble vitamins. In: Gropper SS, Smith JL, Groff JL (eds) Advanced nutrition and human metabolism, 5th edn. Wadsworth, Belmont, pp 373–428

35.

Duque G (2008) Bone and fat connection in aging bone. Curr Opin Rheum 20:429–434CrossRef

36.

Morey E, Baylink DJ (1978) Inhibition of bone formation during space flight. Science 201:1138–1141PubMedCrossRef

37.

Pietschmann P, Skalicky M, Kneissel M, Rauner M, Hofbauer G, Stupphann D, Viidik A (2007) Bone structure and metabolism in a rodent model of male senile osteoporosis. Exp Gerontol 42:1099–1108PubMedCrossRef

38.

Almeida M, Han L, Martin-Millan M, Plotkin LI, Stewart SA, Roberson PK, Kousteni S, O’Brien CA, Bellido T, Parfitt AM, Weinstein RS, Jilka RL, Manolagas SC (2007) Skeletal involution by age-associated oxidative stress and its acceleration by loss of sex steroids. J Biol Chem 282:27285–27297PubMedCentralPubMedCrossRef

39.

Xu H, Watkins BA, Seifert MF (1995) Vitamin E stimulates trabecular bone formation and alters epiphyseal cartilage morphometry. Calcif Tissue Int 57:293–300PubMedCrossRef

40.

la Lastra De, Villegas I (2005) Resveratrol as an anti-inflammatory and anti-aging agent: mechanisms and clinical implications. Mol Nutr Food Res 49:405–430PubMedCrossRef

41.

Franceschi C, Valensin S, Bonafè M, Paolisso G, Yashin AI, Monti D, De Benedictis G (2000) The network and remodeling theories of aging: historical background and new perspectives. Exp Gerontol 35:879–896PubMedCrossRef

42.

Cauley JA, Danielson ME, Boudreau RM, Forrest KY, Zmuda JM, Pahor M, Tylaysky FA, Cummings SR, Harris TB, Newman AB (2007) Inflammatory markers and incident fracture risk in older men and women: the health aging and body composition study. J Bone Miner Res 222:1088–1095CrossRef

43.

Gilbert L, He X, Farmer P, Boden S, Kozlowski M, Rubin J, Nanes MS (2000) Inhibition of osteoblast differentiation by tumor necrosis factor-alpha. Endocrinology 141:3956–3964PubMed

44.

Lorenzo J (2000) Interactions between immune and bone cells: new insights with many remaining questions. J Clin Invest 106:749–752PubMedCentralPubMedCrossRef

45.

Teitelbaum SL (2000) Bone resorption by osteoclasts. Science 289:1504–1508PubMedCrossRef

46.

Weinreb M, Rodan GA, Thompson DD (1989) Osteopenia in the immobilized rat hind limb is associated with increased bone resorption and decreased bone formation. Bone 10:187–194PubMedCrossRef

Titel: Resveratrol supplementation preserves long bone mass, microstructure, and strength in hindlimb-suspended old male rats
verfasst von: Stephanie M. Durbin
Janna R. Jackson
Michael J. Ryan
Joseph C. Gigliotti
Stephan E. Alway
Janet C. Tou
Publikationsdatum: 01.01.2014
Verlag: Springer Japan
Erschienen in: Journal of Bone and Mineral Metabolism / Ausgabe 1/2014
Print ISSN: 0914-8779
Elektronische ISSN: 1435-5604
DOI: https://doi.org/10.1007/s00774-013-0469-2

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Resveratrol supplementation preserves long bone mass, microstructure, and strength in hindlimb-suspended old male rats

Abstract

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Costims – das nächste heiße Ding in der Krebstherapie?

Perioperative Checkpointhemmer-Therapie verbessert NSCLC-Prognose

Positiver FIT: Die Ursache liegt nicht immer im Dickdarm

GLP-1-Agonisten können Fortschreiten diabetischer Retinopathie begünstigen

Update Innere Medizin

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 1/2014

A secular increase in BMD in Chinese women

Erratum to: Bone turnover markers in patients with prostate carcinoma: influence of sex steroids levels

Markers of inflammation after zoledronic acid redosing

Proton concentrations can be a major contributor to the modification of osteoclast and osteoblast differentiation, working independently of extracellular bicarbonate ions

Bone turnover markers in patients with prostate carcinoma: influence of sex steroids levels

The correlation between mineralization degree and bone tissue stiffness in the porcine mandibular condyle

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Costims – das nächste heiße Ding in der Krebstherapie?

Perioperative Checkpointhemmer-Therapie verbessert NSCLC-Prognose

Positiver FIT: Die Ursache liegt nicht immer im Dickdarm

GLP-1-Agonisten können Fortschreiten diabetischer Retinopathie begünstigen

Update Innere Medizin