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01.11.2009 | Original Article

Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts

verfasst von: Carla Andreotti Damante, Giorgio De Micheli, Sueli Patrícia Harumi Miyagi, Ilíria Salomão Feist, Márcia Martins Marques

Erschienen in: Lasers in Medical Science | Ausgabe 6/2009

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Abstract

The effects of laser phototherapy on the release of growth factors by human gingival fibroblasts were studied in vitro. Cells from a primary culture were irradiated twice (6 h interval), with continuous diode laser [gallium–aluminum–arsenium (GaAlAs), 780 nm, or indium–gallium–aluminum–phosphide (InGaAlP),_660 nm] in punctual and contact mode, 40 mW, spot size 0.042 cm², 3 J/cm² and 5 J/cm² (3 s and 5 s, respectively). Positive [10% fetal bovine serum (FBS)] and negative (1%FBS) controls were not irradiated. Production of keratinocyte growth factor (KGF) and basic fibroblast growth factor (bFGF) was quantified by enzyme-linked immunosorbent assay (ELISA). The data were statistically compared by analysis of variance (ANOVA) followed by Tukey’s test (P ≤ 0.05). The characterization of the cell line indicated a mesenchymal nature. KGF release was similar in all groups, while that of bFGF was significantly greater (1.49-times) in groups treated with infra-red laser. It was concluded that increased production of bFGF could be one of the mechanisms by which infra-red laser stimulates wound healing.

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Titel: Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts
verfasst von: Carla Andreotti Damante
Giorgio De Micheli
Sueli Patrícia Harumi Miyagi
Ilíria Salomão Feist
Márcia Martins Marques
Publikationsdatum: 01.11.2009
Verlag: Springer-Verlag
Erschienen in: Lasers in Medical Science / Ausgabe 6/2009
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-008-0582-y

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts

Abstract

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

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