nach oben

Lasers in Medical Science

Erschienen in:

01.05.2012 | Original Article

The effect of noncoherent red light irradiation on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

verfasst von: Fei Peng, Hua Wu, Yadong Zheng, Xiqiang Xu, Jizhe Yu

Erschienen in: Lasers in Medical Science | Ausgabe 3/2012

Einloggen, um Zugang zu erhalten

Abstract

Mesenchymal stem cells (MSCs) are promising for use in regenerative medicine. Low-level light irradiation (LLLI) has been shown to modulate various processes in different biological systems. The aim of our study was to investigate the effect of red light emitted from a light-emitting diode (LED) on bone marrow MSCs with or without osteogenic supplements. MSCs both with and without osteogenic supplements were divided into four groups, and each group was irradiated at doses of 0, 1, 2 and 4 J/cm². Cellular proliferation was evaluated using WST-8 and 5-ethynyl-2′-deoxyuridine (EdU) fluorescence staining. The alkaline phosphatase activity, mineralization, and expression of osteoblast master genes (Col1α1, Alpl, Bglap and Runx2) were monitored as indicators of MSC differentiation towards osteoblasts. In groups without osteogenic supplements, red light at all doses significantly stimulated cellular proliferation, whereas the osteogenic phenotype of the MSCs was not enhanced. In groups with osteogenic supplements, red light increased alkaline phosphatase activity and mineralized nodule formation, and stimulated the expression of Bglap and Runx2, but decreased cellular proliferation. In conclusion, nonconherent red light can promote proliferation but cannot induce osteogenic differentiation of MSCs in normal media, while it enhances osteogenic differentiation and decreases proliferation of MSCs in media with osteogenic supplements.

Prockop DJ (1997) Marrow stromal cells as stem cells for nonhematopoietic tissues. Science 276:71–74PubMedCrossRef

Caplan AI, Bruder S (2001) Mesenchymal stem cells: building blocks for molecular medicine in the 21st Century. Trends Mol Med 7:259–264PubMedCrossRef

Dennis JE, Charbord P (2002) Origin and differentiation of human and murine stroma. Stem Cells 20:205–214PubMedCrossRef

Tondreau T, Lagneaux L, Dejeneffe M, Massy M, Mortier C, Delforge A, Bron D (2004) Bone marrow-derived mesenchymal stem cells already express specific neural proteins before any differentiation. Differentiation 72:319–326PubMedCrossRef

Caplan AI (2005) Review: mesenchymal stem cells: cell-based reconstructive therapy in orthopedics. Tissue Eng 11:1198–1211PubMedCrossRef

Engler AJ, Sen S, Sweeney HL, Discher DE (2006) Matrix elasticity directs stem cell lineage specification. Cell 126:677–689PubMedCrossRef

Liu ZJ, Zhuge Y, Velazquez OC (2009) Trafficking and differentiation of mesenchymal stem cells. J Cell Biochem 106:984–991PubMedCrossRef

Green DE, Longtin JP, Sitharaman B (2009) The effect of nanoparticle-enhanced photoacoustic stimulation on multipotent marrow stromal cells. ACS Nano 3:2065–2072PubMedCrossRef

Mester E, Nagylucskay S, Tisza S, Mester A (1978) Stimulation of wound healing by means of laser rays. Part III – Investigation of the effect on immune competent cells. Acta Chir Acad Sci Hung 19:163–170PubMed

10.

Mester E, Mester AF, Mester A (1985) The biomedical effects of laser application. Lasers Surg Med 5:31–39PubMedCrossRef

11.

Tuby H, Maltz L, Oron U (2007) Low-level laser irradiation (LLLI) promotes proliferation of mesenchymal and cardiac stem cells in culture. Lasers Surg Med 39:373–378PubMedCrossRef

12.

Hou JF, Zhang H, Yuan X, Li J, Wei YJ, Hu SS (2008) In vitro effects of low-level laser irradiation for bone marrow mesenchymal stem cells: proliferation, growth factors secretion and myogenic differentiation. Lasers Surg Med 40:726–733PubMedCrossRef

13.

Pereira AN, Eduardo Cde P, Matson E, Marques MM (2002) Effect of low-power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts. Lasers Surg Med 31:263–267PubMedCrossRef

14.

Kipshidze N, Nikolaychik V, Keelan MH, Shankar LR, Khanna A, Kornowski R, Leon M, Moses J (2001) Low-power helium:neon laser irradiation enhances production of vascular endothelial growth factor and promotes growth of endothelial cells in vitro. Lasers Surg Med 28:355–364PubMedCrossRef

15.

Byrnes KR, Wu X, Waynant RW, Ilev IK, Anders JJ (2005) Low power laser irradiation alters gene expression of olfactory ensheathing cells in vitro. Lasers Surg Med 37:161–171PubMedCrossRef

16.

Karu TI (2003) Low-power laser therapy. In: VoDinh T (ed) Biomedical photonics handbook. CRC Press, Boca Raton, pp 1–25

17.

Oliveira CF, Hebling J, Souza PPC, Sacono NT, Lessa FR, Lizarelli RFZ, Costa CAS (2008) Effect of low-level laser irradiation on odontoblast-like cells. Laser Phys Lett 5:680–685CrossRef

18.

Ozawa Y, Shimizu N, Kariya G, Abiko Y (1998) Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 22:347–354PubMedCrossRef

19.

Abramovitch-Gottlib L, Gross T, Naveh D, Geresh S, Rosenwaks S, Bar I, Vago R (2005) Low level laser irradiation stimulates osteogenic phenotype of mesenchymal stem cells seeded on a three-dimensional biomatrix. Lasers Med Sci 20:138–146PubMedCrossRef

20.

Kim HK, Kim JH, Abbas AA, Kim DO, Park SJ, Chung JY, Song EK, Yoon TR (2009) Red light of 647 nm enhances osteogenic differentiation in mesenchymal stem cells. Lasers Med Sci 24:214–222PubMedCrossRef

21.

Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR, Reyes M, Lenvik T, Lund T, Blackstad M, Du J, Aldrich S, Lisberg A, Low WC, Largaespada DA, Verfaillie CM (2002) Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418:41–49PubMedCrossRef

22.

Horvát-Karajz K, Balogh Z, Kovács V, Drrernat AH, Sréter L, Uher F (2009) In vitro effect of carboplatin, cytarabine, paclitaxel, vincristine, and low-power laser irradiation on murine mesenchymal stem cells. Lasers Surg Med 41:463–469PubMedCrossRef

23.

Peng F, Wu H (2009) Comments on: Red light of 647 nm enhances osteogenic differentiation in mesenchymal stem cells. Lasers Med Sci 24:985–986PubMedCrossRef

24.

Salic A, Mitchison TJ (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci U S A 105:2415–2420PubMedCrossRef

25.

Quarles LD, Yohay LD, Lever LW, Caton R, Wenstrup RJ (1992) Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: an in vitro model of osteoblast development. J Bone Miner Res 7:683–692PubMedCrossRef

26.

Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP (1997) Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem 64:295–312PubMedCrossRef

27.

Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K (2005) Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. Biomaterials 26:3503–3509PubMedCrossRef

28.

Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS (1990) Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 143:420–430PubMedCrossRef

29.

Komori T, Yagi H, Nomura S, Yamaguchi A, Sasaki K, Deguchi K, Shimizu Y, Bronson RT, Gao YH, Inada M, Sato M, Okamoto R, Kitamura Y, Yoshiki S, Kishimoto T (1997) Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 89:755–764PubMedCrossRef

30.

de Souza SC, Munin E, Alves LP, Salgado MA, Pacheco MT (2005) Low power laser radiation at 685 nm stimulates stem-cell proliferation rate in Dugesia tigrina during regeneration. J Photochem Photobiol B 80:203–207PubMedCrossRef

31.

Mvula B, Mathope T, Moore T, Abrahamse H (2008) The effect of low level laser irradiation on adult human adipose derived stem cells. Lasers Med Sci 23:277–282PubMedCrossRef

32.

Posten W, Wrone DA, Dover JS, Arndt KA, Silapunt S, Alam M (2005) Low-level laser therapy for wound healing: mechanism and efficacy. Dermatol Surg 31:334–340PubMedCrossRef

33.

Luo X, Chen J, Song WX, Tang N, Luo J, Deng ZL, Sharff KA, He G, Bi Y, He BC, Bennett E, Huang J, Kang Q, Jiang W, Su Y, Zhu GH, Yin H, He Y, Wang Y, Souris JS, Chen L, Zuo GW, Montag AG, Reid RR, Haydon RC, Luu HH, He TC (2008) Osteogenic BMPs promote tumor growth of human osteosarcomas that harbor differentiation defects. Lab Invest 88:1264–1277PubMedCrossRef

34.

Holleville N, Mateos S, Bontoux M, Bollerot K, Monsoro-Burq AH (2007) Dlx5 drives Runx2 expression and osteogenic differentiation in developing cranial suture mesenchyme. Dev Biol 304:860–874PubMedCrossRef

35.

Komaki M, Karakida T, Abe M, Oida S, Mimori K, Iwasaki K, Noguchi K, Oda S, Ishikawa I (2007) Twist negatively regulates osteoblastic differentiation in human periodontal ligament cells. J Cell Biochem 100:303–314PubMedCrossRef

36.

Komori T (2008) Regulation of bone development and maintenance by Runx2. Front Biosci 13:898–903PubMedCrossRef

37.

Hughes FJ, Collyer J, Stanfield M, Goodman SA (1995) The effects of bone morphogenetic protein-2, -4 and -6 on differentiation of rat osteoblast cells in vitro. Endocrinology 136:2671–2677PubMedCrossRef

38.

Klionsky DJ, Emr SD (1989) Membrane protein sorting: biosynthesis, transport and processing of yeast vacuolar alkaline phosphatase. EMBO J 8:2241–2250PubMed

Titel: The effect of noncoherent red light irradiation on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells
verfasst von: Fei Peng
Hua Wu
Yadong Zheng
Xiqiang Xu
Jizhe Yu
Publikationsdatum: 01.05.2012
Verlag: Springer-Verlag
Erschienen in: Lasers in Medical Science / Ausgabe 3/2012
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-011-1005-z

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

The effect of noncoherent red light irradiation on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

Abstract

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2012

Stimulatory effect of low-level laser therapy on root development of rat molars: a preliminary study

Narrowband ultraviolet B phototherapy and serum folic acid level

Low-level diode laser therapy reduces lipopolysaccharide (LPS)-induced bone cell inflammation

In vitro investigations of repulsion during laser lithotripsy using a pendulum set-up

Efficacy of low-level laser therapy in the management of pain, facial swelling, and postoperative trismus after a lower third molar extraction. A preliminary study

Laser treatment for verrucous hemangioma