Skip to main content
Hauptrubriken
CME Facharzt-Training Webinare Zeitschriften Bücher e.Medpedia Podcast
Service
Jobbörse Info & Hilfe
Fachgebiete
Anästhesiologie Allgemeinmedizin Arbeitsmedizin Augenheilkunde Chirurgie Dermatologie Gynäkologie und Geburtshilfe HNO Innere Medizin Kardiologie Neurologie Onkologie und Hämatologie Orthopädie und Unfallchirurgie Pädiatrie Pathologie Psychiatrie Radiologie Rechtsmedizin Urologie Zahnmedizin
Themen
Typ-2-Diabetes und Adipositas Klimawandel und Gesundheit Neues aus dem Markt COVID-19 Praxis und Beruf Seltene Erkrankungen GOÄ & EBM Panorama
Sammlungen
Kasuistiken Algorithmen & Infografiken Blickdiagnosen Arthropedia FlapFinder (für Dermatochirurgen) Videos Kongressberichterstattung Medizin für Apothekerinnen und Apotheker Für Ärztinnen und Ärzte in Weiterbildung Für Medizinstudierende

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Häufige urologisch-sexualmedizinische Themen in der Praxis sind das Thema des MMW-Webinars am 5. Juni von 17.30 bis 19 Uhr. Konkret geht es um die Frage, wann bei Harnwegsinfektionen auf Antibiotika verzichtet werden kann, und um ein Update zur Therapie von Libido- und Potenzstörungen des Mannes. Der dritte Vortrag behandelt sexuell übertragbare Krankheiten. Stellen Sie Ihre Fragen an die Experten und sammeln Sie 2 CME-Punkte. Jetzt gleich anmelden!
Erweiterte Suche
Anmelden
nach oben
Journal of Assisted Reproduction and Genetics
Erschienen in: Journal of Assisted Reproduction and Genetics 8/2010

01.08.2010 | Assisted Reproduction

Laser assisted zona thinning technique has no beneficial effect on the ART outcomes of two different maternal age groups

verfasst von: Pelin Kutlu, Ozhan Atvar, Omer Faruk Vanlioglu

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 8/2010

Einloggen, um Zugang zu erhalten

Abstract

Purpose

Laser assisted zona thinning is a technique used for facilitating embryo’s hatching process and is commonly used for the embryos of poor prognosis patients with advanced maternal age, repeated implantation failures, poor embryo quality or thick zona pellucida. The aim of this study was to investigate the effect of zona thinning on both good or poor prognosis patients of two different age groups.

Methods

We investigated two different age groups (group 1: <35 years and group 2: ≥35 years ) and compared the effect of assisted zona thinning (groups 1A and 2A) versus not-thinned controls (groups 1B and 2B) in both groups.

Results

The clinical pregnancy rates were 57% and 56% in groups 1A and 1B (p = 0.86), 43% and 38% in groups 2A and 2B (p = 0.59) respectively.

Conclusions

Our results suggest that laser assisted zona thinning of day 3 embryos has no beneficial effect on clinical pregnancy and implantation outcomes.
Literatur
1.
Schiewe MC, Hazeleger NL, Sclimenti C, Balmaceda JP. Physiological characterization of blastocyst hatching mechanisms by use of a mouse antihatching model. Fertil Steril. 1995;63:288–94.PubMed
2.
Cohen J, Elsner C, Kort H, Malter H, Massey J, Mayer MP, et al. Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation. Hum Reprod. 1990;5:7–13.PubMed
3.
Tucker M, Graham J, Han T, Levy M, Sagoskin A, Stillman R. Enhanced implantation of day-6 blastocysts following assisted hatching with acidic tyrode’s medium. Fertil Steril. 1999;72:s20–1.
4.
Blake DA, Forsberg AS, Johansson BR, Wikland M. Laser zona pellucida thinning- an alternative approach to assisted hatching. Hum Reprod. 2001;16:1959–64.CrossRefPubMed
5.
Ali J, Rahbar S, Burjaq H, Sultan AM, Al Flamerzi M, Shahata MA. Routine laser assisted hatching results in significantly increased clinical pregnancies. J Assist Reprod Genet. 2003;20:177–81.CrossRefPubMed
6.
Magli MC, Gianaroli L, Ferraretti AP, Ortini D, Aicardi G, Montanaro N. Rescue of implantation potential in embryos with poor prognosis by assisted zona hatching. Hum Reprod. 1998;13:1331–5.CrossRefPubMed
7.
Cohen J, Alikani M, Trowbridge J, Rosenwaks Z. Implantation enhancement by selective assisted hatching using zona drilling of human embryos with poor prognosis. Hum Reprod. 1992;7:685–91.PubMed
8.
Stein A, Rufas O, Amit S, Avrech O, Pinkas H, Ovadia J, et al. Assisted hatching by partial zona dissection of human pre-embryos in patients with recurrent implantation failure after in vitro fertilization. Fertil Steril. 1995;63:838–41.PubMed
9.
Schoolcraft WB, Schlenker T, Jones GS, Jones Jr HW. In vitro fertilization in women age 40 and older: the impact of assisted hatching. J Assist Reprod Genet. 1995;12:581–4.CrossRefPubMed
10.
Montag M, van der Ven H. Laser-assisted hatching in assisted reproduction. Croat Med J. 1999;40:398–403.PubMed
11.
Meldrum DR, Wisot A, Yee B, Garzo G, Yeo L, Hamilton F. Assisted hatching reduces the age-related decline in IVF outcome in women younger than age 43 without increasing miscarriage or monozygotic twinning. J Assist Reprod Genet. 1998;15:418–21.CrossRefPubMed
12.
Balaban B, Urman B, Alatas C, Mercan R, Mumcu A, Isiklar A. A comparison of four different techniques of assisted hatching. Hum Reprod. 2002;17:1239–43.CrossRefPubMed
13.
Chatzimeletiou K, Picton HM, Handyside AH. Use of a non-contact, infrared laser for zona drilling of mouse embryos: assessment of immediate effects of blastomere viability. Reprod Biomed Online. 2001;2:178–87.PubMed
14.
Hsieh YY, Huang CC, Cheng TC, Chang CC, Tsai HD, Lee MS. Laser-assisted hatching of embryos is better than the chemical method for enhancing the pregnancy rate in women with advanced age. Fertil Steril. 2002;78:179–82.CrossRefPubMed
15.
Malter HE, Cohen J. Blastocyst formation and hatching in vitro following zona drilling of mouse and human embryos. Gamete Res. 1989;24:67–80.CrossRefPubMed
16.
Cohen J, Feldberg D. Effects of the size and number of ZP openings on hatching and trophoblast outgrowth in the Mouse embryo. Mol Reprod Dev. 1991;30:70–80.CrossRefPubMed
17.
Ziebe S, Petersen K, Lindenberg S, Andersen AG, Galrielsen A, Andersen AN. Embryo morphology or cleavage stage: how to select the best embryos for transfer after in-vitro fertilization. Hum Reprod. 1997;12:1545–9.CrossRefPubMed
18.
Sallam HN, Sadek S, Agameya A. Assisted hatching- a meta analysis of randomized controlled trials. J Assist Reprod Genet. 2003;20(8):332–42.CrossRefPubMed
19.
Demirel LC, Evirgen O, Al-Hasani S. The role of assited hatching in human IVF. Middle East Fertil Soc J. 2002;7:6–12.
20.
Edirisinghe WR, Ahnonkitpanit V, Promviengchai S, Suwanjanakorn S, Pruksananonda K, Chinpilas V, et al. A study failing to determine significant benefits from assisted hatching: patients selected for advanced age, zonal thickness of embryos, and previous failed attempts. J Assist Reprod Genet. 1999;16:294–301.CrossRefPubMed
21.
Horng SG, Chang CL, Wu HM, Wang CW, Cheng CK, Huang HY, et al. Laser-assisted hatching of embryos in women of advanced age after in vitro fertilization: a premilimary report. Chang Gung Med J. 2002;25:531–7.PubMed
22.
Hellebaut S, De Sutter P, Dozortsev D, Onghena A, Qian C, Dhont M. Does assisted hatching improve implantation rates after in vitro fertilization or intracytoplasmic sperm injection in all patients? A prospective randomized study. J Assist Reprod Genet. 1996;13:19–22.CrossRefPubMed
23.
Sagoskin A, Levy M, Tucker M, Richter K, Widra E. Laser assisted hatching in good prognosis patients undergoing in vitro fertilization-embryo transfer: a randomized controlled trial. Fertil Steril. 2007;87:283–7.CrossRefPubMed
24.
Silva CP, Silva V, Kommineni K, Keefe D. Effect of in vitro culture of mammalian embryos on the architecture of the zona pellucida. Biol Bull. 1997;193:235–6.PubMed
25.
Carroll J, Depypere H, Matthews CD. Freeze-thaw-induced changes of the zona pellucida explain decreased rates of fertilization in frozen-thawed mouse oocytes. J Reprod Fertil. 1990;90:547–53.CrossRefPubMed
26.
Ge HS, Zhou R, Zhang W, Lin JJ. Impact of assisted hatching on fresh and frozen-thawed embryos: a prospective, randomized study. Reprod Biomed Online. 2008;16(4):589–96.CrossRefPubMed
27.
Huisman GJ, Fauser BC, Eijkemans MJ, Pieters MH. Implantation rates after in vitro fertilization and transfer of a maximum of two embryos that have undergone three to five days in culture. Fertil Steril. 2000;73:117–22.CrossRefPubMed
28.
Fong CY, Bongso A, Sathananthan H, Ho J, Ng SC. Ultrastructural observations of enzymatically treated human blastocysts: zona-free blastocyst transfer and rescue of blastocysts with hatching difficulties. Hum Reprod. 2001;16:540–6.CrossRefPubMed
29.
30.
Cheon YP, Gye MC, Kim CH, Kang BM, Chang YS, Kim SR, et al. Role of actin filaments in the hatching process of mouse blastocysts. Zygote. 1999;7:123–9.CrossRefPubMed
31.
Tucker MJ, Lueeke NM, Wiker SR, Wright G. Chemical removal of the outside of the zona pellucida of day 3 human embryos has no impact on implantation rate. J Assist Reprod Genet. 1993;10:187–91.CrossRefPubMed
32.
Hurst B, Tucker M, Awoniyi A, Schlaff W. Assisted hatching does not enhance IVF success in good prognosis patients. J Assist Reprod Genet. 1998;15:62–4.CrossRefPubMed
33.
Balakier H, Mandel R, Sojecki A, Motamedi G, Zaver S, Librach C. Laser zona thinning in women aged ≤37 years: a randomized study. Fertil Steril. 2009;91:1479–82.CrossRefPubMed
34.
Hagemann A, Lawrence L, Jungheim E, Lanzendorf S, Ratts V, Odem R. Zona pellucida thickness does not predict pregnancy outcomes in patients less than 38 years of age undergoing in vitro fertilization: results from a prospective, randomized trial on assisted hatching. Fertil Steril. 2007;88:s132.CrossRef
35.
Hagemann A, Lanzendorf S, Jungheim E, Chang A, Ratts V, Odem R. A prospective, randomized, double-blinded study of assisted hatching in women younger than 38 years undergoing in vitro fertilization. Fertil Steril. 2010;93:586–91. 34.CrossRefPubMed
36.
Valojerdi M, Eftekhari-Yazdi P, Karimian L, Hassani F, Movaghar B. Effect of laser zona thinning on vitrified–warmed embryo transfer at the cleavage stage: a prospective, randomized study. Reprod Biomed Online. 2010;20:234–42.CrossRefPubMed
Metadaten
Titel
Laser assisted zona thinning technique has no beneficial effect on the ART outcomes of two different maternal age groups
verfasst von
Pelin Kutlu
Ozhan Atvar
Omer Faruk Vanlioglu
Publikationsdatum
01.08.2010
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 8/2010
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-010-9431-6

Weitere Artikel der Ausgabe 8/2010

Journal of Assisted Reproduction and Genetics 8/2010 Zur Ausgabe

fertility preservation

Oocyte cryopreservation: a feasible fertility preservation option for reproductive age cancer survivors

Gonadal Physiology and Disease

Lithium protects against toxic effects of cadmium in the rat testes

Assisted Reproduction

Recent cadmium exposure among male partners may affect oocyte fertilization during in vitro fertilization (IVF)

Gamete biology

Evaluation of Fas positive sperm and complement mediated lysis in subfertile individuals

Gamete biology

Effect of α-tocopherol supplementation on in vitro maturation of sheep oocytes and in vitro development of preimplantation sheep embryos to the blastocyst stage

Assisted Reproduction

Anti-mullerian hormone and cumulative pregnancy outcome in in-vitro fertilization

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Mammakarzinom: Brustdichte beeinflusst rezidivfreies Überleben

26.05.2024 Mammakarzinom Nachrichten

Frauen, die zum Zeitpunkt der Brustkrebsdiagnose eine hohe mammografische Brustdichte aufweisen, haben ein erhöhtes Risiko für ein baldiges Rezidiv, legen neue Daten nahe.

Mehr Lebenszeit mit Abemaciclib bei fortgeschrittenem Brustkrebs?

24.05.2024 Mammakarzinom Nachrichten

In der MONARCHE-3-Studie lebten Frauen mit fortgeschrittenem Hormonrezeptor-positivem, HER2-negativem Brustkrebs länger, wenn sie zusätzlich zu einem nicht steroidalen Aromatasehemmer mit Abemaciclib behandelt wurden; allerdings verfehlte der numerische Zugewinn die statistische Signifikanz.

Blutdrucksenkung könnte Uterusmyome verhindern

21.05.2024 Krebsvorsorge in der Gynäkologie Nachrichten

Frauen mit unbehandelter oder neu auftretender Hypertonie haben ein deutlich erhöhtes Risiko für Uterusmyome. Eine Therapie mit Antihypertensiva geht hingegen mit einer verringerten Inzidenz der gutartigen Tumoren einher.

„Übersichtlicher Wegweiser“: Lauterbachs umstrittener Klinik-Atlas ist online

17.05.2024 Klinik aktuell Nachrichten

Sie sei „ethisch geboten“, meint Gesundheitsminister Karl Lauterbach: mehr Transparenz über die Qualität von Klinikbehandlungen. Um sie abzubilden, lässt er gegen den Widerstand vieler Länder einen virtuellen Klinik-Atlas freischalten.

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen
Bildnachweise