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06.06.2020 | Original Research

Decrease of miR-125a-5p in Gastritis and Gastric Cancer and Its Possible Association with H. pylori

verfasst von: Mônica Pezenatto dos Santos, Jéssica Nunes Pereira, Roger Willian De Labio, Lilian Carla Carneiro, Jaqueline Correia Pontes, Mônica Santiago Barbosa, Marília De Arruda Cardoso Smith, Spencer Luíz Marques Payão, Lucas Trevizani Rasmussen

Erschienen in: Journal of Gastrointestinal Cancer | Ausgabe 2/2021

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Abstract

Purpose

The aim of this study was to evaluate the expression of miR-125a-5p in patients with dyspeptic symptoms and gastric cancer, correlating them with the development of this cancer and H. pylori.

Methods

Patients were divided in groups according to histopathological analysis (control, gastritis, and cancer groups). Polymerase chain reaction was performed to detect H. pylori and real-time quantitative PCR to determine miR-125a-5p expression.

Results

H. pylori was detected in 44% of the patients, with prevalence in the gastritis and cancer groups. A statistically significant decrease of miR-125a-5p expression was found in the control positive (p = 0.0183*), gastritis positive (p = 0.0380*), and cancer positive (p = 0.0288*) groups when compared with the control negative group.

Conclusion

We suggest that decreased expression of the miRNA-125a-5p associated with the presence of the H. pylori is an important mechanism in gastric diseases and could be a possible marker for early diagnosis of gastric cancer.

Strong VE. Progress in gastric cancer. Updat Surg. 2018;70:157–9. https://doi.org/10.1007/s13304-018-0543-3 [Internet]. Springer International Publishing.CrossRef

Cai M, Chen Q, Shen J, Lv C, Cai L. Epigenetic silenced miR-125a-5p could be self-activated through targeting Suv39h1 in gastric cancer. J Cell Mol Med. 2018;22:4721–31. https://doi.org/10.1111/jcmm.13716.CrossRefPubMedPubMedCentral

Pei Y, Tang Z, Cai M, Yao Q, Xie B, Zhang X. The E2F3/miR-125a/DKK3 regulatory axis promotes the development and progression of gastric cancer. Cancer Cell Int. 2019;19:1–12. https://doi.org/10.1186/s12935-019-0930-y [Internet]. BioMed Central.CrossRef

Zhang Z, Dong Y, Hua J, Xue H, Hu J, Jiang T, et al. A five-miRNA signature predicts survival in gastric cancer using bioinformatics analysis. Gene. 2019;699:125–34. https://doi.org/10.1016/j.gene.2019.02.058 [Internet]. Elsevier B.V.CrossRefPubMed

Sabbagh P, Javanian M, Koppolu V, Vasigala VKR, Ebrahimpour S. Helicobacter pylori infection in children: an overview of diagnostic methods. Eur J Clin Microbiol Infect Dis. 2019;38:1035–45. https://doi.org/10.1007/s10096-019-03502-5.CrossRefPubMed

Cao Y, Tan S, Tu Y, Zhang G, Liu Y, Li D, et al. MicroRNA-125a-5p inhibits invasion and metastasis of gastric cancer cells by targeting BRMS1 expression. Oncol Lett. 2018;15:5119–30. https://doi.org/10.3892/ol.2018.7983.CrossRefPubMedPubMedCentral

Sonkoly E, Pivarcsi A. Advances in microRNAs: implications for immunity and inflammatory diseases. J Cell Mol Med. 2009;13:24–38. https://doi.org/10.1111/j.1582-4934.2008.00534.x.

Rossi AFT, Cadamuro ACT, Biselli-Périco JM, Leite KRM, Severino FE, Reis PP, et al. Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection. Cell Microbiol. 2016;18:1444-58. https://doi.org/10.1111/cmi.12587.CrossRefPubMedPubMedCentral

Noto JM, Peek RM. The role of microRNAs in Helicobacter pylori pathogenesis and gastric carcinogenesis. Front Cell Infect Microbiol. 2012;1:1-19. https://doi.org/10.3389/fcimb.2011.00021.CrossRef

10.

Hashiguchi Y, Nishida N, Mimori K, Sudo T, Tanaka F, Shibata K, et al. Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance. Int J Oncol. 2012;40:1477-82. https://doi.org/10.3892/ijo.2012.1363.CrossRefPubMed

11.

Hou L, Luo P, Ma Y, Jia C, Yu F, Lv Z, et al. MicroRNA-125a-3p downregulation correlates with tumorigenesis and poor prognosis in patients with non-small cell lung cancer. Oncol Lett. 2017;14:4441-8. https://doi.org/10.3892/ol.2017.6809.CrossRefPubMedPubMedCentral

12.

Stolte M, Meining A. The updated Sydney system: classification and grading of gastritis as the basis of diagnosis and treatment. Can J Gastroenterol. 2001;15:591-8. https://doi.org/10.1155/2001/367832.CrossRefPubMed

13.

Scholte GH, van Doorn LJ, Quint WG, Lindeman J. Polymerase chain reaction for the detection of Helicobacter pylori in formaldehyde-sublimate fixed, paraffin-embedded gastric biopsies. Diagn Mol Pathol. 1997;6:238-43. https://doi.org/10.1097/00019606-199708000-00008.CrossRefPubMed

14.

Santos M, Sallas L, Zapparoli D, Orcini WA, Chen E, Smith M, et al. Lack of association between IL-6 polymorphisms and haplotypes with gastric cancer. 2018;120:9448-54. https://doi.org/10.1002/jcb.28220.

15.

Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods. 2001;25:402-8. https://doi.org/10.1006/met.2001.1262.CrossRefPubMed

16.

Ishimoto T, Baba H, Izumi D, Sugihara H, Kurashige J, Iwatsuki M, et al. Current perspectives toward the identification of key players in gastric cancer microRNA dysregulation. Int J Cancer. 2016;138:1337-49. https://doi.org/10.1002/ijc.29627.CrossRefPubMed

17.

Sallas ML, Melchiades JL, Zabaglia LM, do Prado Moreno JR, Orcini WA, Chen ES, et al. Prevalence of Helicobacter pylori vacA, cagA, dupA and oipA genotypes in patients with gastric disease. Adv Microbiol. 2017;07:1-9. https://doi.org/10.4236/aim.2017.71001.CrossRef

18.

Wang F, Meng W, Wang B, Qiao L. Helicobacter pylori-induced gastric inflammation and gastric cancer. Cancer Lett. 2014;345:196-202. https://doi.org/10.1016/j.canlet.2013.08.016 [Internet]. Elsevier Ireland Ltd.CrossRefPubMed

19.

Yamaoka Y. Roles of the plasticity regions of Helicobacter pylori in gastroduodenal pathogenesis. J Med Microbiol. 2008;57:545-53. https://doi.org/10.1099/jmm.0.2008/000570-0.CrossRefPubMedPubMedCentral

20.

Zheng Q, Chen C, Guan H, Kang W, Yu C. Prognostic role of microRNAs in human gastrointestinal cancer: a systematic review and meta-analysis. Oncotarget. 2017;8:46611-23. https://doi.org/10.18632/oncotarget.16679.CrossRefPubMedPubMedCentral

21.

Zhong L, Sun S, Shi J, Cao F, Han X, Chen Z. MicroRNA-125a-5p plays a role as a tumor suppressor in lung carcinoma cells by directly targeting STAT3. Tumor Biol. 2017;39:101042831769757. https://doi.org/10.1177/1010428317697579.CrossRef

22.

Park Y, Kim J. Regulation of IL-6 signaling by miR-125a and let-7e in endothelial cells controls vasculogenic mimicry formation of breast cancer cells. BMB Rep. 2019;52:214-9. https://doi.org/10.5483/BMBRep.2019.52.3.308.CrossRefPubMedPubMedCentral

23.

Wu WY, Xue XY, Chen ZJ, Han SL, Huang YP, Zhang LF, et al. Potentially predictive microRNAs of gastric cancer with metastasis to lymph node. World J Gastroenterol. 2011;17:3645-51. https://doi.org/10.3748/wjg.v17.i31.3645.CrossRefPubMedPubMedCentral

24.

Wang JK, Wang Z, Li G. MicroRNA-125 in immunity and cancer. Cancer Lett. 2019;454:134-45. https://doi.org/10.1016/j.canlet.2019.04.015 [Internet]. Elsevier.CrossRefPubMed

25.

Dai J, Wang J, Yang L, Xiao Y, Ruan Q. MIR-125a regulates angiogenesis of gastric cancer by targeting vascular endothelial growth factor A. Int J Oncol. 2015;47:1801-10. https://doi.org/10.3892/ijo.2015.3171.CrossRefPubMed

26.

Jo HN, Kang H, Lee A, Choi J, Chang W, Lee MS, et al. Endothelial miR-26a regulates VEGF-Nogo-B receptor-mediated angiogenesis. BMB Rep. 2017;50:384-9. https://doi.org/10.5483/bmbrep.2017.50.7.085.CrossRefPubMedPubMedCentral

27.

Lu G, Ma Y, Jia C, Yang H, Xie R, Luo P, et al. Reduced miR-125a levels associated with poor survival of patients with hepatocellular cancer. Oncol Lett. 2017;14:5952-8. https://doi.org/10.3892/ol.2017.6902.

28.

Sun M, Guo S, Yao J, Xiao Y, Sun R, Ma W, et al. MicroRNA-125a suppresses cell migration, invasion, and regulates hyaluronic acid synthase 1 expression by targeting signal transducers and activators of transcription 3 in renal cell carcinoma cells. J Cell Biochem. 2019;120:1894-902. https://doi.org/10.1002/jcb.27503.CrossRefPubMed

29.

Kiselev FL. MicroRNA and cancer. Mol Biol (Mosk). 2014;48:232-42. https://doi.org/10.1016/j.molonc.2012.09.006 [Internet]. Elsevier B.V.CrossRef

Titel: Decrease of miR-125a-5p in Gastritis and Gastric Cancer and Its Possible Association with H. pylori
verfasst von: Mônica Pezenatto dos Santos
Jéssica Nunes Pereira
Roger Willian De Labio
Lilian Carla Carneiro
Jaqueline Correia Pontes
Mônica Santiago Barbosa
Marília De Arruda Cardoso Smith
Spencer Luíz Marques Payão
Lucas Trevizani Rasmussen
Publikationsdatum: 06.06.2020
Verlag: Springer US
Erschienen in: Journal of Gastrointestinal Cancer / Ausgabe 2/2021
Print ISSN: 1941-6628
Elektronische ISSN: 1941-6636
DOI: https://doi.org/10.1007/s12029-020-00432-w

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Decrease of miR-125a-5p in Gastritis and Gastric Cancer and Its Possible Association with H. pylori