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Journal of Endocrinological Investigation

Erschienen in:

01.05.2017 | Original Article

Graves’ orbitopathy, idiopathic orbital inflammatory pseudotumor and Epstein–Barr virus infection: a serological and molecular study

verfasst von: M. Leo, F. Maggi, G. R. Dottore, G. Casini, P. Mazzetti, M. Pistello, S. Sellari-Franceschini, M. Nardi, P. Vitti, C. Marcocci, M. Marinò

Erschienen in: Journal of Endocrinological Investigation | Ausgabe 5/2017

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Abstract

Objective

One of the hypotheses on the pathogenesis of autoimmune diseases, including Graves’ disease (GD) and Graves’ orbitopathy (GO), involves bacterial or viral infections. Recently, Epstein–Barr virus (EBV) has been proposed to play a role in the pathogenesis of idiopathic orbital inflammatory pseudotumor (IOIP) in Asians. The aim of the present study was to investigate the possible association of GO with EBV infection/exposure, as compared with IOIP, using serum and tissue samples, as well as primary cultures of orbital fibroblasts.

Methods

Thirty-one patients were studied, including four with IOIP, ten with GO, nine with GD without GO and eight control patients without IOIP, GD and GO. All patients with IOIP and GO underwent orbital decompression. Control patients underwent palpebral surgery. Fibroadipose orbital tissue samples were collected. Serum anti-EBV antibodies were measured in all patients. EBV-DNA was measured in blood samples, orbital tissue samples and primary cultures of orbital fibroblasts.

Results

Serum assays showed that the vast majority of patients have had a previous exposure to EBV, but no one had an acute infection. EBV-DNA was detected in ~40% of blood samples from GO, GD and control patients, but in none of the IOIP samples. EBV-DNA was not detected in any of the orbital tissue samples tested or in primary cultures of orbital fibroblasts.

Conclusions

EBV infection does not seem to be associated with GD, GO and IOIP in Caucasians. Whether EBV is involved in IOIP in Asians or other populations remains to be confirmed.

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Titel: Graves’ orbitopathy, idiopathic orbital inflammatory pseudotumor and Epstein–Barr virus infection: a serological and molecular study
verfasst von: M. Leo
F. Maggi
G. R. Dottore
G. Casini
P. Mazzetti
M. Pistello
S. Sellari-Franceschini
M. Nardi
P. Vitti
C. Marcocci
M. Marinò
Publikationsdatum: 01.05.2017
Verlag: Springer International Publishing
Erschienen in: Journal of Endocrinological Investigation / Ausgabe 5/2017
Elektronische ISSN: 1720-8386
DOI: https://doi.org/10.1007/s40618-016-0587-5

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Graves’ orbitopathy, idiopathic orbital inflammatory pseudotumor and Epstein–Barr virus infection: a serological and molecular study