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Inflammation Research
Erschienen in: Inflammation Research 10/2009

01.10.2009 | Original Research Paper

Heme oxygenase-1 mediates the anti-allergic actions of quercetin in rodent mast cells

verfasst von: Miyoko Matsushima, Kenzo Takagi, Miyuki Ogawa, Etsuko Hirose, Yui Ota, Fumie Abe, Kenji Baba, Takaaki Hasegawa, Yoshinori Hasegawa, Tsutomu Kawabe

Erschienen in: Inflammation Research | Ausgabe 10/2009

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Abstract

Objective and design

We investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of quercetin against degranulation of rat basophilic leukemia (RBL-2H3) cells, rat peritoneal mast cells, and mouse bone marrow-derived mast cells.

Methods

The strength of allergic reaction was evaluated by the extent of degranulation in mast cells sensitized with various stimulants. The levels of HO-1, HO-2, and nuclear factor erythroid 2-related factor 2 (Nrf2) expressions were determined by quantitative RT-PCR, western blotting, or immunocytochemistry.

Results

Heme oxygenase activity was upregulated after short exposure to quercetin, followed by the induction of HO-1 expression after long exposure to quercetin. The inhibition of degranulation by quercetin was reversed using tin protoporphyrin IX (SnPP), an HO-1 inhibitor. HO-1 metabolites, bilirubin and CO, led to inhibit degranulation, and quercetin translocated Nrf2 from cytoplasm into nucleus in RBL-2H3 cells.

Conclusion

These results strongly suggest that quercetin exerted anti-allergic actions via activation of Nrf2-HO-1 pathway.
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Metadaten
Titel
Heme oxygenase-1 mediates the anti-allergic actions of quercetin in rodent mast cells
verfasst von
Miyoko Matsushima
Kenzo Takagi
Miyuki Ogawa
Etsuko Hirose
Yui Ota
Fumie Abe
Kenji Baba
Takaaki Hasegawa
Yoshinori Hasegawa
Tsutomu Kawabe
Publikationsdatum
01.10.2009
Verlag
SP Birkhäuser Verlag Basel
Erschienen in
Inflammation Research / Ausgabe 10/2009
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-009-0039-1

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