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Inflammation Research
Erschienen in: Inflammation Research 11-12/2018

11.10.2018 | Original Research Paper

LncRNA MEG3 impacts proliferation, invasion, and migration of ovarian cancer cells through regulating PTEN

verfasst von: Juelan Wang, Wenqian Xu, Yangke He, Qi Xia, Siwei Liu

Erschienen in: Inflammation Research | Ausgabe 11-12/2018

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Abstract

Objective and design

We investigated the expressions of lncRNA MEG3 and PTEN in ovarian cancer tissues and their effects on cell proliferation, cycle and apoptosis of ovarian cancer.

Methods

Expression levels of MEG3 in ovarian cancer cell lines and normal ovarian cell lines were detected by qRT-PCR. Cell viability was detected by MTT assay. Cell apoptosis and cell cycle distribution were measured by flow cytometry. Cell invasion capability was tested by transwell assay. Cell migration capacity was tested by wound healing. The xenograft model was constructed to explore the effect of lncRNA MEG3 on ovarian cancer in vivo.

Result

Compared with normal ovarian cells, expression levels of MEG3 and PTEN were relatively lower in ovarian cancer cells. There was a positive correlation between the expression of PTEN and the expression of MEG3. Enhanced expression level of PTEN suppressed SKOV3 cell proliferation, increased cell apoptosis rate, and decreased cell invasion and migration.

Conclusion

LncRNA MEG3 and PTEN were down-regulated in ovarian cancer cells. LncRNA MEG3 regulated the downstream gene PTEN in ovarian cancer cells to prohibit cell proliferation, promote apoptosis and block cell cycle progression.
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Metadaten
Titel
LncRNA MEG3 impacts proliferation, invasion, and migration of ovarian cancer cells through regulating PTEN
verfasst von
Juelan Wang
Wenqian Xu
Yangke He
Qi Xia
Siwei Liu
Publikationsdatum
11.10.2018
Verlag
Springer International Publishing
Erschienen in
Inflammation Research / Ausgabe 11-12/2018
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-018-1186-z

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