nach oben

European Journal of Nutrition

Erschienen in:

01.03.2009 | ORIGINAL CONTRIBUTION

Carnosic acid reduces cytokine-induced adhesion molecules expression and monocyte adhesion to endothelial cells

verfasst von: Ya-Mei Yu, PhD, MPH, Chin-Huei Lin, MS, Hsu-Chin Chan, PhD, Hong-Der Tsai, MD

Erschienen in: European Journal of Nutrition | Ausgabe 2/2009

Einloggen, um Zugang zu erhalten

Abstract

Background

Expression of cell adhesion molecules on the endothelium and the attachment of monocytes to endothelium may play a major role in the early atherogenic process.

Aim of the study

We investigated the effects of carnosic acid on the adhesion of U937 cells to IL-1β-treated human umbilical vein endothelial cells (HUVECs), as well as on the expression of adhesion molecules.

Results

Our data showed that pretreatment with 10 and 20 µmol/l carnosic acid significantly reduced the number of U937 cells adhering to IL-1β-treated HUVECs. In addition, we found that 20 µmol/l carnosic was more effective than 10 µmol/l carnosic acid at inhibiting expression of cell adhesion molecules (ICAM-1, VCAM-1, and E-selectin), the nuclear translocation of NF-κB subunits p65 and p50, and the production of ROS in IL-1β-stimulated HUVECs.

Conclusions

We conclude that carnosic acid inhibits IL-1β-induced ICAM-1, VCAM-1 and E-selectin expression in HUVECs through a mechanism that involves NFκB. We propose that the reduction in binding of human monocytic cell line U937 to IL-1β-treated HUVECs is due to the anti-inflammatory properties of carnosic acid.

Alexiou D, Karayiannakis AJ, Syrigos KN, Zbar A, Kremmyda A, Bramis I, Tsigris C (2001) Serum levels of E-selectin, ICAM-1 and VCAM-1 in colorectal cancer patients: correlations with clinicopathological features, patient survival and tumour surgery. Eur J Cancer 37:2392–2397CrossRef

Aruoma OI, Halliwell B, Aeschbach R, Löliger J (1992) Antioxidant and pro-oxidant properties of active rosemary constituents: carnosol and carnosic acid. Xenobiotica 22:257–268CrossRef

Baldwin A (1996) The NF-κB and IκB proteins: new discoveries and insights. Annu Rev Immunol 14:649–683CrossRef

Cybulsky MA, Gimbrone MA, Jr (1991) Endothelial expression of a mononuclear leukocyte adhesion molecule during atherogenesis. Science 251:788–791CrossRef

Dschietzig T, Richter C, Pfannenschmidt G, Bartsch C, Laule M, Baumann G, Stangl K (2001) Dexamethasone inhibits stimulation of pulmonary endothelins by proinflammatory cytokines: possible involvement of a nuclear factor kappa B dependent mechanism. Intensive Care Med 27:751–756CrossRef

Haraguchi H, Saito T, Okamura N, Yagi A (1995) Inhibition of lipid peroxidation and superoxide generation by diterpenoids from Rosmarinus officinalis. Plant Med 61:333–336CrossRef

Harrison D, Griendling KK, Landmesser U, Hornig B, Drexler H (2003) Role of oxidative stress in atherosclerosis. Am J Cardiol 91:7A–11ACrossRef

Ilja CW, Peter CH (2005) Polyphenols and disease risk in epidemiologic studies. Am J Clin Nutr 81:317S–325S

Islam KN, Devaraj S, Jialal I (1998) Alpha-tocopherol enrichment of monocytes decreases agonist-induced adhesion to human endothelial cells. Circulation 98:2255–2261

10.

Kiyofumi N, Hisashi M, Hirosh S, Norihisa N, Naokik T, Tomoe Y, Toshi M, Masayuki Y (2004) Carnosic acid, a new class of lipid absorption inhibitor from saga. Bioorg Med Chem Lett 14:1943–1946CrossRef

11.

Lenardo MJ, Baltimore D (1989) NF-κB: a pleiotropic mediator of inducible and tissue-specific gene control. Cell 58:227–229CrossRef

12.

Ludwig A, Lorenz M, Grimbo N, Steinle F, Meiners S, Bartsch C, Stangl K, Baumann G, Stangl V (2004) The tea flavonoid epigallocatechin-3-gallate reduces cytokine-induced VCAM-1 expression and monocyte adhesion to endothelial cells. Biochem Biophys Res Commun 316:659–665CrossRef

13.

Manna SK, Zhang HJ, Yan T, Oberley LW, Aggarwal BB (1998) Overexpression of manganese superoxide dismutase suppresses tumor necrosis factor-induced apoptosis and activation of nuclear transcription factor-kappa B and activated protein-1. J Biol Chem 273:13245–13254CrossRef

14.

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63CrossRef

15.

Murase T, Kume N, Hase T, Yusuke S, Yoshinori N, Ichiro T, Toru K (1999) Gallates inhibit cytokine-induced nuclear translocation of NF-κB and expression of leukocyte adhesion molecules in vascular endothelial cells. Arterioscler Thromb Vasc Biol 19:1412–1420

16.

Price DT, Loscalzo J (1999) Cellular adhesion molecules and atherogenesis. Am J Med 107:85–97CrossRef

17.

Ross R (1999) Atherosclerosis is an inflammatory disease. Am Heart J 138:419–420CrossRef

18.

Shishodia S, Potdar P, Gairola CG, Aggarwal BB (2003) Curcumin (diferuloylmethane) downregulates cigarette smoke-induced NF-kappaB activation through inhibition of IkappaBalpha kinase in human lung epithelial cells: correlation with suppression of COX-2, MMP-9 and cyclin D1. Carcinogenesis 24:1269–1279CrossRef

19.

Schreck R, Albermann K, Baeuerle PA (1992) Nuclear factor kappa B: an oxidative stress-responsive transcription factor of eukaryotic cells. Free Radic Res Com 17:221–237CrossRef

20.

Silvina BL, Balz F (2006) Dietary flavonoids attenuate tumor necrosis factor-α induced adhesion molecule expression in human aortic endothelial cells. J Biol Chem 281:37102–37110CrossRef

21.

Suzuki YJ, Packer L (1993) Inhibition of NF-κB activation by vitamin E derivatives. Biochem Biophys Res Commun 193:227–283CrossRef

22.

Tak PP, Firestein GS (2001) NF-κB: a key role in inflammatory diseases. J Clin Invest 107:7–11CrossRef

23.

True AL, Rahman A, Malik AB (2000) Activation of NF-kappa B induced by H₂O₂ and TNF-alpha and its effects on ICAM-1 expression in endothelial cells. Am J Physiol Lung Cell Mol Physiol 279:302–311

24.

Udalova IA, Richardson A, Denys A, Smith C, Ackerman H, Foxwell B, Kwiatkowski D (2000) Functional consequences of a polymorphism affecting NF-kappa B p50–p50 binding to the TNF promoter region. Mol Cell Biol 20:9113–9119CrossRef

25.

Valen G, Yan ZQ, Hansson GK (2001) Nuclear factor kappa-B and the heart. J Am Coll Cardiol 38:307–314CrossRef

26.

Wang H, Joseph JA (1999) Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic Biol Med 27:612–616CrossRef

27.

Yu YM, Wang ZH, Liu CH, Chen CS (2007) Ellagic acid inhibits IL-1β-induced cell adhesion molecule expression in HUVECs. Br J Nutr 7:692–698CrossRef

28.

Yu YM, Lin HC, Chang WC (2008) Carnosic acid prevents the migration of human aortic smooth muscle cells by inhibiting the activation and expression of MMP-9. Br J Nutr 100:731–738CrossRef

Titel: Carnosic acid reduces cytokine-induced adhesion molecules expression and monocyte adhesion to endothelial cells
verfasst von: Ya-Mei Yu, PhD, MPH
Chin-Huei Lin, MS
Hsu-Chin Chan, PhD
Hong-Der Tsai, MD
Publikationsdatum: 01.03.2009
Verlag: Steinkopff-Verlag
Erschienen in: European Journal of Nutrition / Ausgabe 2/2009
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-008-0768-x

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Notfall-TEP der Hüfte ist auch bei 90-Jährigen machbar

26.04.2024 Hüft-TEP Nachrichten

Ob bei einer Notfalloperation nach Schenkelhalsfraktur eine Hemiarthroplastik oder eine totale Endoprothese (TEP) eingebaut wird, sollte nicht allein vom Alter der Patientinnen und Patienten abhängen. Auch über 90-Jährige können von der TEP profitieren.

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 Hypotonie Nachrichten

Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.

Bei schweren Reaktionen auf Insektenstiche empfiehlt sich eine spezifische Immuntherapie

25.04.2024 Allergien und Intoleranzreaktionen Nachrichten

Insektenstiche sind bei Erwachsenen die häufigsten Auslöser einer Anaphylaxie. Einen wirksamen Schutz vor schweren anaphylaktischen Reaktionen bietet die allergenspezifische Immuntherapie. Jedoch kommt sie noch viel zu selten zum Einsatz.

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

25.04.2024 Hypertonie Nachrichten

Beginnen ältere Männer im Pflegeheim eine Antihypertensiva-Therapie, dann ist die Frakturrate in den folgenden 30 Tagen mehr als verdoppelt. Besonders häufig stürzen Demenzkranke und Männer, die erstmals Blutdrucksenker nehmen. Dafür spricht eine Analyse unter US-Veteranen.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Background

Aim of the study

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2009

Cholecystokinin secretion induced by β-conglycinin peptone depends on Gαq-mediated pathways in enteroendocrine cells

Effects of high and normal soyprotein breakfasts on satiety and subsequent energy intake, including amino acid and ‘satiety’ hormone responses

Allium vegetable intake and risk of acute myocardial infarction in Italy

Effects of seafood consumption and weight loss on fasting leptin and ghrelin concentrations in overweight and obese European young adults

Dietary calcium and vitamin D2 supplementation with enhanced Lentinula edodes improves osteoporosis-like symptoms and induces duodenal and renal active calcium transport gene expression in mice

Effects of soy isoflavone consumption on bone structure and milk mineral concentration in a rat model of lactation-associated bone loss