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Archives of Gynecology and Obstetrics

Erschienen in:

01.09.2014 | Gynecologic Oncology

MicroRNA-543 suppresses endometrial cancer oncogenicity via targeting FAK and TWIST1 expression

verfasst von: Li Bing, Chen Hong, Shang Li-Xin, Gao Wei

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 3/2014

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Abstract

Introduction

Focal adhesion kinase (FAK) is a critical mediator of extracellular matrix signaling, cell survival, proliferation and motility. Twist homolog 1 (TWIST1) is a transcription factor and serves as a powerful oncogene. Increased FAK and TWIST1 expression are observed in a variety of solid human tumors and correlate with metastasis and poor survival.

Materials and methods

Here, we identify miR-543 as a direct regulator of FAK and TWIST1 expression in endometrial cancer.

Results

Forced expression of miR-543 in endometrial cancer cell lines decreases both endogenous FAK and TWIST1 mRNA and protein levels. Forced expression of miR-543 in aggressive endometrial cancer cell lines also impairs tumor cell monolayer proliferation, anchorage-independent growth, migration and invasion. Endogenous miR-543 expression is decreased in malignant versus normal endometrium tissue and the levels of miR-543 inversely correlate with mRNA levels of FAK and TWIST1.

Conclusions

miR-543 expression is decreased in endometrial cancer and serves as a tumor suppressor by targeting FAK and TWIST1 expression.

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Titel: MicroRNA-543 suppresses endometrial cancer oncogenicity via targeting FAK and TWIST1 expression
verfasst von: Li Bing
Chen Hong
Shang Li-Xin
Gao Wei
Publikationsdatum: 01.09.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 3/2014
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-014-3219-3

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Springer Medizin

MicroRNA-543 suppresses endometrial cancer oncogenicity via targeting FAK and TWIST1 expression

Abstract

Introduction

Materials and methods

Results

Conclusions

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Springer Medizin

Abstract

Introduction

Materials and methods

Results

Conclusions

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