nach oben

International Journal of Legal Medicine

Erschienen in:

01.01.2006 | Short Communication

Y-chromosomal STR haplotypes in a Northeast Italian population sample using 17plex loci PCR assay

verfasst von: Stefania Turrina, Renzo Atzei, Domenico De Leo

Erschienen in: International Journal of Legal Medicine | Ausgabe 1/2006

Einloggen, um Zugang zu erhalten

Abstract

One hundred fifty-five unrelated, autochthonous healthy males from Northeast Italy were typed for the 17 Y-chromosome short tandem repeat (STR) (Y-STR) loci DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385, DYS393, DYS391, DYS439, DYS635, DYS392, Y GATA H4, DYS437, DYS438, DYS448 using the AmpFLSTR Yfiler polymerase chain reaction amplification kit. A total of 153 different haplotypes were observed, and among these, 151 were unique, while 2 were found two times. The overall haplotype diversity was 0.9997. Furthermore, 50 father–son pairs, previously confirmed by autosomal STR analysis, were typed using the same set of 17 Y-STR loci, and, among 850 allele transfers, three mutation events were identified, giving an average mutation rate of 3.53×10⁻³ per locus per generation (95% confidence interval 0.73–1.03).

Nur mit Berechtigung zugänglich

Berger B, Lindinger A, Niederstätter H, Grubwieser P, Parson W (2005) Y-STR typing of an Austrian population sample using a 17-loci multiplex PCR assay. Int J Legal Med. DOI 10.1007/s0041400505468

Bosch E, Lee AC, Calafell F, Arroyo E, Henneman P, De Knijff P, Jobling MA (2002) High resolution Y chromosome typing: 19 STRs amplified in three multiplex reactions. Forensic Sci Int 125:42–51CrossRefPubMed

Dupuy BM, Andreassen R, Flones AG et al (2001) Y chromosome variation in a Norwegian population sample. Forensic Sci Int 117:163–173CrossRefPubMed

Gusmão L, Butler ML, Carracedo A et al (2005) DNA Commission of the International Society of Forensic Genetics (ISFG): an update of the recommendations on the use of Y-STRs in forensic analysis. Int J Legal Med. DOI 10.1007/s0041400500261

Hall A, Ballantyne J (2003) Novel Y-STR typing strategies reveal the genetic profile of the semen donor in extended interval post-coital cervicovaginal samples. Forensic Sci Int 136:58–72CrossRefPubMed

Kayser M, Caglià A, Corache D et al (1997) Evaluation of Y-chromosomal STRs: a multicenter study. Int J Legal Med 110:125–133CrossRefPubMed

Kayser M, Roewer L, Hedman M et al (2000) Characteristics and frequency of germline mutations at microsatellite loci from human Y chromosome, as revealed by direct observation in father/son pairs. Am J Hum Genet 66:1580–1588CrossRefPubMed

Kurihara R, Yamamoto T, Uchihi R et al (2004) Mutations in 14 Y-STR loci among Japanese father–son haplotypes. Int J Legal Med 118:125–131CrossRefPubMed

Parson W, Niederstätter H, Kochl S, Steinlechner M, Berger B (2001) When autosomal tandem repeats fail: optimized primer and reaction design for Y-chromosome short tandem repeat analysis in forensic casework. Croat Med J 42:285–287PubMed

10.

Redd AJ, Agellon AB, Kearney VA, Contreras VA et al (2002) Forensic value of 14 novel STRs on the human Y chromosome. Forensic Sci Int 130:97–111CrossRefPubMed

11.

Schoske R, Vallone PM, Kline MC, Redman JW, Butler JM (2004) High-throughput Y-STR typing of U.S. populations with 27 regions of the Y chromosome using two multiplex PCR assays. Forensic Sci Int 139:107–121CrossRefPubMed

12.

Sibille I, Duverneuil C, De La Grandmaison GL, Guerrouache K, Teissière F, Durigon M, De Mazancourt P (2002) Y-STR DNA amplification as biological evidence in sexually assaulted female victims with no cytological detection of spermatozoa. Forensic Sci Int 125:212–216CrossRefPubMed

13.

Tang JP, Hou YP, Li YB, Wu J, Zhang J, Zhang HJ (2003) Characterization of eight Y-STR loci and haplotypes in a Chinese Han population. Int J Legal Med 117:263–270CrossRefPubMed

14.

Uchihi R, Yamamoto T, Usuda K et al (2003) Haplotype analysis with 14 Y-STR loci using 2 multiplex amplification and typing systems in 2 regional populations in Japan. Int J Legal Med 117:34-38PubMed

Titel: Y-chromosomal STR haplotypes in a Northeast Italian population sample using 17plex loci PCR assay
verfasst von: Stefania Turrina
Renzo Atzei
Domenico De Leo
Publikationsdatum: 01.01.2006
Verlag: Springer-Verlag
Erschienen in: International Journal of Legal Medicine / Ausgabe 1/2006
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-005-0054-x

Springer Medizin

Y-chromosomal STR haplotypes in a Northeast Italian population sample using 17plex loci PCR assay

Abstract

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 1/2006

Reference data for the Thiemann–Nitz method of assessing skeletal age for the purpose of forensic age estimation

An unusual case of homicidal near drowning followed by electrocution

Age estimation in children by measurement of open apices in teeth

The influence of slice thickness on assessment of clavicle ossification in forensic age diagnostics

Allele frequencies for 15 STR loci in a population from the Republic of Macedonia

Effective strategies for forensic analysis in the mitochondrial DNA coding region

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie