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01.05.2006 | Original Article

Subtyping mtDNA haplogroup H by SNaPshot minisequencing and its application in forensic individual identification

verfasst von: P. Grignani, G. Peloso, A. Achilli, C. Turchi, A. Tagliabracci, M. Alù, G. Beduschi, U. Ricci, L. Giunti, C. Robino, S. Gino, C. Previderè

Erschienen in: International Journal of Legal Medicine | Ausgabe 3/2006

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Abstract

Sequence variation of the hypervariable segments (HVS) I/II of mitochondrial DNA (mtDNA) and the haplogroup affiliation were determined in a sample of 271 Italian subjects. This analysis showed that 42% of the individuals could be ascribed to H, the most frequent haplogroup in European Caucasian populations. This fraction was then screened for specific single nucleotide polymorphisms located in the coding region to identify H subclades H1–H15. We set up two multiplex polymerase chain reactions and specific SNaPshot assays to investigate the frequency distribution of these subgroups in our population sample and to examine their usefulness in discriminating among commonly shared HVS I/II sequences. This allowed the assignment of a large portion of the mtDNAs (∼70%) to specific subhaplogroups, with H1 and H5 being the most represented. About two-thirds of the individuals sharing common HVS I/II sequences were subdivided and ascribed to specific H subhaplogroups with a significant reduction of the frequencies of the most common mtDNA haplotypes. Haplogroup H subtyping could thus be extremely useful in forensic identification when many samples have to be analysed and compared, avoiding excessive time-consuming and labor-intensive sequencing analysis.

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Titel: Subtyping mtDNA haplogroup H by SNaPshot minisequencing and its application in forensic individual identification
verfasst von: P. Grignani
G. Peloso
A. Achilli
C. Turchi
A. Tagliabracci
M. Alù
G. Beduschi
U. Ricci
L. Giunti
C. Robino
S. Gino
C. Previderè
Publikationsdatum: 01.05.2006
Verlag: Springer-Verlag
Erschienen in: International Journal of Legal Medicine / Ausgabe 3/2006
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-005-0059-5

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Subtyping mtDNA haplogroup H by SNaPshot minisequencing and its application in forensic individual identification

Abstract

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Live-Webinar "Chronische Unterbauch- und Viszeralschmerzen in der Praxis managen"

Springer Medizin

Abstract

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