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International Journal of Legal Medicine

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01.03.2007 | Technical Note

Y-STR analysis of degraded DNA using reduced-size amplicons

verfasst von: Myung Jin Park, Hwan Young Lee, Ukhee Chung, Seung-Chul Kang, Kyoung-Jin Shin

Erschienen in: International Journal of Legal Medicine | Ausgabe 2/2007

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Abstract

To increase the success rate of Y-STR genotyping for degraded DNA, we have developed two multiplex PCR sets for 21 Y-STR loci. Besides the 17 Y-STR loci of DYS19, DYS385, DYS389-I, DYS389-II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, and GATA H4.1 contained in a commercial Y-STR kit, AmpFlSTR® Yfiler™, the other four loci of DYS388, DYS446, DYS447, and DYS449 were also included in the multiplexes to increase the discrimination capacity. Among a total of 21 Y-STR loci, the primers for eight loci (DYS385, DYS390, DYS438, DYS446, DYS448, DYS449, and DYS635) were newly designed in the present study and nine loci (DYS385, DYS390, DYS391, DYS392, DYS438, DYS439, DYS448, and DYS635) have PCR amplicons smaller than those of the AmpFlSTR® Yfiler™ kit. A sensitivity test using serially diluted standard 9948 male DNA showed that all the values of Y-STR loci in the Y-miniplexes are reliable at template concentrations as low as 30 pg. We compared the effectiveness of the two multiplexes with the AmpFlSTR® Yfiler™ kit by using both enzymatically degraded DNA and 30 samples of 50-year-old skeletal remains. This comparison demonstrated that the new Y-miniplex sets can produce a better signal from degraded DNA than the AmpFlSTR® Yfiler™ kit.

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Titel: Y-STR analysis of degraded DNA using reduced-size amplicons
verfasst von: Myung Jin Park
Hwan Young Lee
Ukhee Chung
Seung-Chul Kang
Kyoung-Jin Shin
Publikationsdatum: 01.03.2007
Verlag: Springer-Verlag
Erschienen in: International Journal of Legal Medicine / Ausgabe 2/2007
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-006-0133-7

Springer Medizin

Y-STR analysis of degraded DNA using reduced-size amplicons

Abstract

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

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