Introduction
Materials and methods
DNA extraction
DNA quantification
STR typing of nuclear DNA
MtDNA sequencing
Statistical analysis
Results and discussion
Bone sample | Maximum quantity (pg/µl) | Autosomal STR loci | Y-STR loci | mtDNA |
---|---|---|---|---|
Femur A ant | 221 | 18/18 | 17/17 | HVI, HVII |
Femur B ant | 32 | 18/18 | 7/17 | HVI, HVII |
Femur C ant | 60 | 17/18 | 17/17 | HVI, HVII |
Femur D ant | 37 | 18/18 | 17/17 | HVI, HVII |
Femur E ant | 70 | 18/18 | 17/17 | HVI, HVII |
Femur F ant | 50 | 18/18 | 14/17 | HVI, HVII |
Femur G ant | 40 | 17/18 | 12/17 | HVI, HVII |
Femur H ant | 106 | 18/18 | 17/17 | HVI, HVII |
Femur I ant | 26 | 17/18 | 17/17 | HVI, HVII |
R Femur 43 pat | 237 | 18/18 | 17/17 | HVI, HVII |
R Femur 45 pat | 46 | 17/18 | 14/17 | HVI, HVII |
R Femur 49 pat | 116 | 18/18 | 17/17 | HVI, HVII |
R Femur 51 pat | 56 | 18/18 | 17/17 | HVI, HVII |
R Femur 1 | 62 | 18/18 | 16/17 | HVI, HVII |
R Femur 2 | 10 | 13/18 | 12/17 | |
R Femur 3 | 43 | 18/18 | 16/17 | HVI, HVII |
R Femur 4 | 21 | 17/18 | 16/17 | HVI, HVII |
R Femur 5 | 18 | 16/18 | 14/17 | HVI, HVII |
R Femur 6 | 10 | 14/18 | 7/17 | HVII |
R Femur 7 | 24 | 18/18 | 17/17 | HVI, HVII |
R Femur 8 | 69 | 18/18 | 16/17 | HVI, HVII |
R Femur 9 | 17 | 18/18 | 16/17 | HVI, HVII |
R Femur 10 | 46 | 18/18 | 16/17 | HVI, HVII |
R Femur 11 | 114 | 18/18 | 17/17 | HVI, HVII |
R Femur 12 | 120 | 18/18 | 17/17 | HVI, HVII |
R Femur 13 | 35 | 18/18 | 17/17 | HVI, HVII |
R Femur 14 | 60 | 16/18 | 13/17 | HVI, HVII |
R Femur 15 | 100 | 18/18 | 17/17 | HVI, HVII |
R Femur 16 | 51 | 18/18 | 8/17 | HVI, HVII |
R Femur 17 | 110 | 18/18 | 17/17 | HVI, HVII |
R Femur 18 | 1,000 | 18/18 | 17/17 | HVI, HVII |
R Femur 19 | 270 | 18/18 | 17/17 | HVI, HVII |
R Femur 20 | 15 | 11/18 | 14/17 | HVI, HVII |
R Femur 21 | 142 | 18/18 | 17/17 | HVI, HVII |
R Femur 22 | 111 | 18/18 | 17/17 | HVI, HVII |
R Femur 23 | 130 | 18/18 | 17/17 | HVI, HVII |
R Femur 24 | 75 | 18/18 | 17/17 | HVI, HVII |
R Femur 25 | 36 | 18/18 | 17/17 | HVI, HVII |
R Femur 26 | 39 | 16/18 | 15/17 | HVI, HVII |
R Femur 27 | 150 | 18/18 | 16/17 | HVI, HVII |
R Femur 28 | 41 | 18/18 | 17/17 | HVI, HVII |
R Femur 29 | 48 | 15/18 | 6/17 | HVI, HVII |
R Femur 30 | 64 | 18/18 | 17/17 | HVI, HVII |
R Femur 31 | 17 | 16/18 | 13/17 | HVI, HVII |
R Femur 32 | 42 | 18/18 | 17/17 | HVI, HVII |
R Femur 33 | 100 | 18/18 | 17/17 | HVI, HVII |
R Femur 34 | 62 | 18/18 | 16/17 | HVI, HVII |
R Femur 35 | 64 | 18/18 | 10/17 | HVI, HVII |
R Femur 36 | 77 | 16/18 | 16/17 | HVI, HVII |
R Femur 37 | 280 | 18/18 | 17/17 | HVI, HVII |
R Femur 38 | 130 | 18/18 | 17/17 | HVI, HVII |
R Femur 39 | 150 | 18/18 | 17/17 | HVI, HVII |
R Femur 40 | 30 | 17/18 | 17/17 | |
R Femur 41 | 50 | 18/18 | 17/17 | HVI, HVII |
R Femur 42 | 43 | 17/18 | 12/17 | HVI, HVII |
R Femur 43 | 8 | 14/18 | 14/17 | HVI, HVII |
R Femur 44 | 26 | 17/18 | 17/17 | HVI, HVII |
R Femur 45 | 57 | 18/18 | 17/17 | HVI, HVII |
R Femur 46 | 47 | 24/24 | 7/17 | HVI, HVII |
R Femur 47 | 77 | 18/18 | 17/17 | HVII |
R Femur 48 | 100 | 18/18 | 17/17 | HVI, HVII |
R Femur 49 | 101 | 18/18 | 10/17 | HVI, HVII |
R Femur 50 | 90 | 18/18 | 9/17 | HVI, HVII |
R Femur 51 | 47 | 18/18 | 7/17 | HVI, HVII |
R Femur 52 | 18 | 18/18 | 17/17 | HVI, HVII |
R Femur 53 | 91 | 18/18 | 8/17 | HVI, HVII |
R Femur 54 | 14 | 14/18 | 16/17 | HVI, HVII |
R Femur 55 | 120 | 18/18 | 10/17 | HVI, HVII |
R Femur 56 | 109 | 18/18 | 17/17 | HVI, HVII |
R Femur 57 | 73 | 18/18 | 10/17 | HVI, HVII |
R Femur 58 | 48 | 17/18 | 17/17 | HVI, HVII |
R Femur 59 | 47 | 18/18 | 9/17 | HVI, HVII |
R Femur 60 | 24 | 18/18 | 16/17 | HVI, HVII |
R Femur 61 | 250 | 18/18 | 17/17 | HVI, HVII |
R Femur 62 | 33 | 18/18 | 14/17 | HVI, HVII |
R Femur 63 | 17 | 17/18 | 16/17 | HVI, HVII |
R Femur 64 | 18 | 18/18 | 15/17 | HVI, HVII |
R Femur 65 | 4 | 0/18 | 0/17 | HVI, HVII |
R Femur 66 | 36 | 18/18 | 17/17 | HVI, HVII |
R Femur 67 | 34 | 18/18 | 17/17 | HVI, HVII |
R Femur 68 | 5 | 0/18 | 0/17 | HVI, HVII |
R Femur 69 | 93 | 18/18 | 7/17 | HVI, HVII |
R Femur 70 | 15 | 22/24 | 11/17 | HVI, HVII |
R Femur 71 | 51 | 17/18 | 16/17 | HVI, HVII |
Bone | Reference | LR(n-STR)
| PP(n-STR)
| LR(mtDNA)
| LR(Y-STR)
|
---|---|---|---|---|---|
Femur D anta
| Sister | 4.1 × 109
| 99.999998% | 2.2 × 103
| |
R Femur 11a
| Sister | 4.8 × 106
| 99.998% | 2.2 × 103
| |
Femur E ant | Sister | 1.8 × 104
| 99.5% | 2.2 × 103
| |
R Femur 43 pat | Sister | 6.8 × 102
| 87.3% | 1.5 × 103
| |
R Femur 66 | Sister | 8.0 × 103
| 98.8% | 2.2 × 103
| |
R Femur 9 | Sister | 1.4 × 103
| 93.4% | 2.2 × 103
| |
Femur B ant | Sister | 3.9 × 104
| 99.7% | 1.7 × 102
| |
R Femur 20 | Sister | 1.9 × 102
| 65.0% | 2.2 × 103
| |
R Femur 26 | Sister | 2.6 × 103
| 96.3% | 2.2 × 103
| |
R Femur 30 | Sister | 6.7 × 103
| 98.7% | 7.5 × 101
| |
R Femur 42 | Sister | 3.7 × 103
| 97.4% | 2.2 × 103
| |
R Femur 44 | Sister | 1.6 × 105
| 99.94% | 2.2 × 103
| |
R Femur 52 | Sister | 5.7 × 105
| 99.98% | 1.5 × 102
| |
R Femur 61 | Sister | 1.9 × 104
| 99.5% | 2.2 × 103
| |
R Femur 34 | Cousin | 5.8 × 101
| 37.0% | 2.2 × 103
| |
R Femur 46 | Nephew | 4.0 × 101
| 28.8% | 2.2 × 103
| |
R Femur 49 pat | Brother | 2.3 × 104
| 99.6% | 2.2 × 103
| 2.7 × 103
|
Femur G ant | Brother | 2.6 × 103
| 96.3% | 2.2 × 103
| 5.9 × 102
|
R Femur 14 | Brother | 2.3 × 102
| 70.0% | 5.6 × 102
| 2.7 × 103
|
R Femur 18 | Brother | 2.7 × 105
| 99.96% | 9.0 × 102
| 2.7 × 103
|
R Femur 19 | Brother | 3.0 × 105
| 99.97% | 7.5 × 102
| 7.6 × 102
|
R Femur 43 | Brother | 3.8 × 103
| 97.5% | 1.5 × 103
| 2.7 × 103
|
R Femur 40 | Son | 9.2 × 104
| 99.9% | 2.7 × 103
| |
R Femur 23 | Daughter and wife | 3.7 × 106
| 99.997% | ||
R Femur 41 | Daughter | 5.9 × 105
| 99.98% | ||
R Femur 48 | Daughter | 3.8 × 105
| 99.97% | ||
R Femur 53 | Daughter | 2.5 × 105
| 99.96% | ||
R Femur 70 | Daughter | 4.6 × 105
| 99.98% |
Bone | Reference | LR(n-STR x mtDNA)
| PP(n-STR x mtDNA)
| LR(n-STR x Y-STR)
| PP(n-STR x Y-STR)
|
---|---|---|---|---|---|
Femur D anta
| Sister | 9.2 × 1012
| >99.999999% | ||
R Femur 11a
| Sister | 1.1 × 1010
| >99.999999% | ||
Femur E ant | Sister | 4.0 × 107
| 99.9998% | ||
R Femur 43 pat | Sister | 1.0 × 106
| 99.99% | ||
R Femur 66 | Sister | 1.8 × 107
| 99.9995% | ||
R Femur 9 | Sister | 3.2 × 106
| 99.997% | ||
Femur B ant | Sister | 6.7 × 106
| 99.999% | ||
R Femur 20 | Sister | 4.2 × 105
| 99.97% | ||
R Femur 26 | Sister | 5.9 × 106
| 99.998% | ||
R Femur 30 | Sister | 5.0 × 105
| 99.98% | ||
R Femur 42 | Sister | 8.4 × 106
| 99.999% | ||
R Femur 44 | Sister | 3.7 × 108
| 99.99997% | ||
R Femur 52 | Sister | 8.5 × 107
| 99.9999% | ||
R Femur 61 | Sister | 4.2 × 107
| 99.9998% | ||
R Femur 34 | Cousin | 1.3 × 105
| 99.92% | ||
R Femur 46 | Nephew | 8.9 × 104
| 99.9% | ||
R Femur 49 pat | Brother | 5.1 × 107
| 99.9998% | 6.2 × 107
| 99.9998% |
Femur G ant | Brother | 5.8 × 106
| 99.998% | 1.5 × 106
| 99.994% |
R Femur 14 | Brother | 1.3 × 105
| 99.92% | 6.2 × 105
| 99.98% |
R Femur 18 | Brother | 2.4 × 108
| 99.99996% | 7.3 × 108
| 99.99998% |
R Femur 19 | Brother | 2.2 × 108
| 99.99996% | 2.3 × 108
| 99.99996% |
R Femur 43 | Brother | 5.7 × 106
| 99.998% | 1.0 × 107
| 99.999% |
R Femur 40 | Son | 2.5 × 108
| 99.99996% |