Immunohistochemical analysis on aquaporin-1 and aquaporin-3 in skin wounds from the aspects of wound age determination

Immunohistochemical investigation of aquaporin (AQP)1 and AQP3 was performed in human skin wounds obtained from forensic autopsy cases. A total of 55 human skin wounds of different postinfliction intervals were collected as follows: group I, 0–3 days (n = 16); II, 4–7 days (n = 11); III, 9–14 days (n = 16); and IV, 17–21 days (n = 12). In uninjured skin samples, AQP1 and AQP3 could be slightly detected in dermal vessels and keratinocytes, respectively. The percentage of AQP1⁺ vessels and the number of AQP3⁺ keratinocytes were apparently elevated in accordance with wound ages. The number of AQP3⁺ keratinocytes was distinctly evident in groups II and III. Morphometrically, both AQP1⁺ vessel area and AQP3⁺ cell number were markedly increased in group II, compared with other three groups. With regard to forensic safety, AQP1⁺ vessel area of over 5% would imply wound ages of 4–12 days. Moreover, the positive area of > 15% would suggest wound age of 7–10 days. Especially, most samples of skin wounds aged 5–10 days except for only one sample (a 10-day-old wound) showed AQP3⁺ cell number of > 300, and the remaining other samples had that of < 300. Thus, the AQP3⁺ cell number of > 300 would indicate wound ages of 5–10 days. Collectively, immunohistochemical analyses of AQP1 and AQP3 in human skin wounds would support the objective accuracy of wound age determination.