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01.09.2010 | Original Article

Obliterative airway remodelling in transplanted and non-transplanted lungs

verfasst von: Danny Jonigk, Katharina Theophile, Kais Hussein, Oliver Bock, Ulrich Lehmann, Clemens L. Bockmeyer, Jens Gottlieb, Stefan Fischer, Andre Simon, Tobias Welte, Lavinia Maegel, Hans Kreipe, Florian Laenger

Erschienen in: Virchows Archiv | Ausgabe 3/2010

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Abstract

Obliterative airway remodelling is a morphological sequence in a variety of pulmonary diseases. Notably, bronchiolitis obliterans represents one of the key complications of lung transplantation, induced by (immigrating) myofibroblasts. A comparative expression analysis of obliterative airway remodelling in transplanted and non-transplanted patients has not been reported so far. Obliterated and unremodelled airways from explanted lungs (n = 19) from patients suffering from chronic allograft dysfunction, infection, graft-versus-host disease and toxic exposure were isolated by laser-assisted microdissection. Airways from lung allografts harvested shortly before and after transplantation (n = 4) as well as fibroblastic foci from lungs with interstitial pulmonary fibrosis (n = 4) served as references. Pre-amplified cDNA was analysed by quantitative real-time RT-PCR for expression of fibrosis, inflammation and apoptosis-associated genes. Composition of infiltrating cells and protein expression were assessed by conventional histology and immunohistochemistry. Bronchiolitis obliterans in transplanted patients showed a significant increase of BMP-7 expression (p = 0.0141 compared with controls), while TGF-β1 and FGF-2 as well as BMP-4 and BMP-7 were up-regulated in fibroblastic foci in interstitial pulmonary fibrosis (p < 0.0424 compared with controls). Regarding other fibrosis-associated genes (BMP-6, SMAD-3, CASP-3 and CASP-9, FASLG, NF-KB1, IL-1 and IL-2) as well as cellularity and cellular composition, no significant differences between obliterative airway remodelling in transplanted and non-transplanted patients could be shown. Obliterative airway remodelling in lung allografts and in non-transplanted patients share many morphological and genetic traits. BMPs, especially BMP-7, warrant further investigation as possible markers for the aggravation of airway remodelling.

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Titel: Obliterative airway remodelling in transplanted and non-transplanted lungs
verfasst von: Danny Jonigk
Katharina Theophile
Kais Hussein
Oliver Bock
Ulrich Lehmann
Clemens L. Bockmeyer
Jens Gottlieb
Stefan Fischer
Andre Simon
Tobias Welte
Lavinia Maegel
Hans Kreipe
Florian Laenger
Publikationsdatum: 01.09.2010
Verlag: Springer-Verlag
Erschienen in: Virchows Archiv / Ausgabe 3/2010
Print ISSN: 0945-6317
Elektronische ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-010-0949-x

Live-Webinar "Chronische Unterbauch- und Viszeralschmerzen in der Praxis managen"

Springer Medizin

Obliterative airway remodelling in transplanted and non-transplanted lungs

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Live-Webinar "Chronische Unterbauch- und Viszeralschmerzen in der Praxis managen"

Springer Medizin

Abstract

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