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01.06.2011 | Original Article

Claudins and tricellulin in fibrolamellar hepatocellular carcinoma

verfasst von: Attila Patonai, Boglárka Erdélyi-Belle, Anna Korompay, Áron Somorácz, Beate K. Straub, Peter Schirmacher, Ilona Kovalszky, Gábor Lotz, András Kiss, Zsuzsa Schaff

Erschienen in: Virchows Archiv | Ausgabe 6/2011

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Abstract

Fibrolamellar hepatocellular carcinoma is a subtype of hepatocellular carcinoma occurring in non-cirrhotic liver at a younger age. The tumor expresses both hepatocellular and cholangiocellular markers. Previously, our group described overexpression of tight junction protein claudin 4 in cholangiocellular carcinoma in contrast to hepatocellular carcinoma. In the present study, tight junction protein expressions were studied to possibly clarify bipotential lineage of fibrolamellar hepatocellular carcinoma. Eleven fibrolamellar hepatocellular carcinomas were compared with seven “conventional” hepatocellular carcinomas, seven cholangiocellular carcinomas, and five normal liver samples. By immunohistochemistry, all fibrolamellar hepatocellular carcinomas were positive for HepPar1 and cytokeratins 7, 8, and 18, but negative for cytokeratin 19. Glypican-3 gave weak staining in two cases. Expression of claudin 1 was lower, while that of claudin 2 was higher in fibrolamellar hepatocellular carcinomas than in other tumors. Claudins 3, 4, and 7 were not detectable in fibrolamellar hepatocellular carcinomas as in the majority of “conventional” hepatocellular carcinomas, contrary to high expression observed in cholangiocellular carcinomas. Focal or diffuse claudin 5 expression was detected in nine of 11 fibrolamellar hepatocellular carcinomas contrary to other tumors. Tricellulin was significantly downregulated in all tumors compared with normal liver. Our findings showed claudins to exhibit specific expression patterns in fibrolamellar hepatocellular carcinomas not observed in other primary liver tumors, with unique claudin 5 expression and pattern features similar to common hepatocellular carcinoma, but different from cholangiocellular carcinoma. This is the first report describing the loss of tricellulin expression in human hepatic tumors.

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Titel: Claudins and tricellulin in fibrolamellar hepatocellular carcinoma
verfasst von: Attila Patonai
Boglárka Erdélyi-Belle
Anna Korompay
Áron Somorácz
Beate K. Straub
Peter Schirmacher
Ilona Kovalszky
Gábor Lotz
András Kiss
Zsuzsa Schaff
Publikationsdatum: 01.06.2011
Verlag: Springer-Verlag
Erschienen in: Virchows Archiv / Ausgabe 6/2011
Print ISSN: 0945-6317
Elektronische ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-011-1077-y

Springer Medizin

Claudins and tricellulin in fibrolamellar hepatocellular carcinoma

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

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