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Journal of Cancer Research and Clinical Oncology
Erschienen in: Journal of Cancer Research and Clinical Oncology 4/2007

01.04.2007 | Original Paper

Transcription factor SOX2 up-regulates stomach-specific pepsinogen A gene expression

verfasst von: Yasuyo Tani, Yoshimitsu Akiyama, Hiroshi Fukamachi, Kazuyoshi Yanagihara, Yasuhito Yuasa

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 4/2007

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Abstract

Purpose

Transcription factor SOX2 is expressed in normal gastric mucosae but not in the normal colon. We aimed to clarify the role of SOX2 with reference to pepsinogen expression in the gastrointestinal epithelium.

Methods

We analyzed expression of SOX2 and pepsinogens, differentiation markers of the stomach, in ten gastric cancer (GC) and ten colorectal cancer (CRC) cell lines. The effects of over-expression and down-regulation of SOX2 on pepsinogen expression were also examined.

Results

Six GC and five CRC cell lines showed SOX2 expression on RT-PCR. Expression of pepsinogen A was detectable in eight GC and seven CRC cell lines, whereas the majority of the cell lines expressed pepsinogen C. Over-expression of SOX2 up-regulated expression of pepsinogen A but not that of pepsinogen C in 293T human embryonic kidney cells, and some GC and CRC cell lines. Moreover, pepsinogen A expression was significantly reduced by SOX2 RNA interference in two GC cell lines.

Conclusion

These data suggest that SOX2 plays an important role in regulation of pepsinogen A, and ectopic expression of SOX2 may be associated with abnormal differentiation of colorectal cancer cells.
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Metadaten
Titel
Transcription factor SOX2 up-regulates stomach-specific pepsinogen A gene expression
verfasst von
Yasuyo Tani
Yoshimitsu Akiyama
Hiroshi Fukamachi
Kazuyoshi Yanagihara
Yasuhito Yuasa
Publikationsdatum
01.04.2007
Verlag
Springer-Verlag
Erschienen in
Journal of Cancer Research and Clinical Oncology / Ausgabe 4/2007
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI
https://doi.org/10.1007/s00432-006-0165-x

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