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Journal of Cancer Research and Clinical Oncology

Erschienen in:

01.06.2009 | Original Paper

Inhibitor of growth 4 induces apoptosis in human lung adenocarcinoma cell line A549 via Bcl-2 family proteins and mitochondria apoptosis pathway

verfasst von: Xiaomei Li, Qingyuan Zhang, Limin Cai, Yanhua Wang, Qian Wang, Xiaoyi Huang, Songbin Fu, Jing Bai, Jinglei Liu, Guangmei Zhang, Jiping Qi

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 6/2009

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Abstract

Objective

Inhibitor of growth 4 (ING4) is considered to be a tumor suppressor implicated in several human malignancies by tumor growth inhibition and apoptosis enhancement. In present study, the effects of ING4 on apoptosis and its mechanisms were investigated through the transduction of ING4 cDNA into lung adenocarcinoma cell line A549.

Methods

The effects of ING4 on A549 apoptosis were observed by FCM analysis, TUNEL assay, and electron microscopy. Simultaneously, the effects of ING4 on the expression of several apoptosis-related proteins in cell line A549 were evaluated by Western blot analysis.

Results

Both Annexin-V FITC analysis by FCM and TUNEL assay revealed more apoptotic cells in A549 cells with exogenous ING4 gene. For electron microscopy, A549 cells with exogenous ING4 gene showed typical morphological changes of apoptosis. The deregulation of Bcl-2 family proteins (Bcl-2, Bcl-xl, Bax, Bak, Bid) and the major apoptotic executioners of mitochondria pathway (Cyt-c, caspase3, PARP) were also observed.

Conclusion

Our findings suggest that exogenous ING4 can enhance A549 apoptosis via regulating the expression of Bcl-2 family proteins and the activation of mitochondrial apoptotic pathway.

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Titel: Inhibitor of growth 4 induces apoptosis in human lung adenocarcinoma cell line A549 via Bcl-2 family proteins and mitochondria apoptosis pathway
verfasst von: Xiaomei Li
Qingyuan Zhang
Limin Cai
Yanhua Wang
Qian Wang
Xiaoyi Huang
Songbin Fu
Jing Bai
Jinglei Liu
Guangmei Zhang
Jiping Qi
Publikationsdatum: 01.06.2009
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 6/2009
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-008-0519-7

Springer Medizin

Inhibitor of growth 4 induces apoptosis in human lung adenocarcinoma cell line A549 via Bcl-2 family proteins and mitochondria apoptosis pathway