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Journal of Cancer Research and Clinical Oncology

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01.05.2010 | Original Paper

Chemopreventive effect of bacoside A on N-nitrosodiethylamine-induced hepatocarcinogenesis in rats

verfasst von: Panneerselvam Janani, Kanakarajan Sivakumari, Arumugam Geetha, Baskaran Ravisankar, Chandrakesan Parthasarathy

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 5/2010

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Abstract

Purpose

Chemoprevention is an effective approach to control hepatocarcinogenesis. Bacoside A, the active constituent of Bacopa monniera Linn., is anticipated to play a role in chemoprevention of liver cancer.

Methods

In the present study, we investigated the chemopreventive effect of bacoside A against N-nitrosodiethylamine-induced hepatocarcinogenesis in an animal model.

Results

Administration of carcinogen showed a significant elevation in the levels of lipid peroxidation, serum tumor marker enzymes and liver injury marker enzymes with subsequent decrease in the levels of both hemolysate and liver antioxidant status. Bacoside A co-treatment maintained the N-nitrosodiethylamine-induced alterations at near normal level. Histopathological and electron microscopic study of the liver tissue also supports the above biochemical observations.

Conclusions

From our findings we conclude that bacoside A is effective to prevent DEN-induced hepatocellular carcinoma by quenching lipid peroxidation and enhancing antioxidant status through free radical scavenging mechanism and having potential of protecting endogenous enzymatic and non-enzymatic antioxidant activity.

Abelev GI (1971) Alpha-fetoprotein in ontogenesis and its association with malignant tumors. Adv Cancer Res 14:295–358CrossRefPubMed

Anonymous (1998) Indian herbal pharmacopoeia, vol 1. RRL, Jammu Tawi/IDMA, Mumbai, pp 30–37

Banakar MC, Paramasivan SK, Chattopadhyay MB, Datta S, Chakraborty P, Chatterjee M, Kannan K, Thyagarajan E (2004) 1α, 25-dihydroxyvitamin D3 prevents DNA damage and restores antioxidant enzymes in rat hepatocarcinogenesis induced by diethylnitrosamine and promoted by phenobarbitol. World J Gastroenterol 10(9):1268–1275PubMed

Bannasch P, Zerban H (1990) Pathology of tumours in laboratory animals. Tumours of the rat. Tumours of the liver. IARC Sci Publ (99):199–240

Barretto OC, Zyngier SB (1984) Acquired disturbance of erythrocyte glutathione reductase in experimental tumors. J Cancer Res Clin Oncol 108:252–253CrossRefPubMed

Batko J, Warchol T, Karon H (1996) The activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase in erythrocytes of rats with experimental neoplastic diseases. Acta Biochim Pol 43:403–405PubMed

Beauchamp CO, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287CrossRefPubMed

Bergmeyer HV, Gowehn K, Grassel M (1974) Methods of enzymatic analysis. Academic Press, New york, p 438

Bhattacharya SK, Bhattacharya A, Kumar A, Ghosal S (2000) Antioxidant activity of Bacopa monniera in rat frontal cortex, striatum and hippocampus. Phytother Res 14:174–179CrossRefPubMed

Bulle F, Mavier P, Zafrani ES, Preaux AM, Lescs MC, Siegrist S, Dhumeaux D, Guellaen G (1990) Mechanism of γ-glutamyl transpeptidase release in serum during intrahepatic and extrahepatic cholestasis in the rat: a histochemical, biochemical and molecular approach. Hepatology 11:545–550CrossRefPubMed

Buzby GP, Mullen JH, Stein TP, Rosato EF (1980) Host tumor interactions and nutrient supply. Cancer 45:2940–2947CrossRefPubMed

Channa S, Dar A, Yaqoob M, Anjum S, Sultani Z, Rahman A (2003) Bronchovasodilatory activity of fractions of pure constituents isolated from Bacopa monnieri. J Ethnopharmacol 86:27–35CrossRefPubMed

Channa S, Dar A, Anjum S, Yaqoob M, Rahman A (2006) Anti inflammatory activity of Bacopa monniera in rodents. J Ethanopharmacol 104:286–289CrossRef

Chopra RN, Nayar SL, Chopra IC (1956) Glossary of Indian medicinal plants. CSIR, New Delhi

Clemens MR, Walker HD (1987) Lipid peroxidation in erythrocytes. Chem Phys Lipids 45:251–268CrossRefPubMed

D’souza P, Deepak M, Rani P, Kadamboor S, Mathew A, Chandrashekar AP, Agarwal A (2002) Brine shrimp lethality assay of Bacopa monnieri. Phytother Res 16:197–198CrossRefPubMed

Dagli MLZ (2002) Agentes antineoplasicos. In: Spinosa HS, Gorniak SL, Bernardi MM (eds) Farmacologia aplicada á medicina veterinaria, third ed. Editora Guanabara-Koogan, São Paulo, Brasil, pp 577–608

Daubeuf S, Leroy P, Paolicchi A, Pompella A, Wellman M, Galteau MM, Visvikis A (2002) Enhanced resistance of HeLa cells to cisplatin by overexpression of gamma-glutamyltransferase. Biochem Pharmacol 64(2):207–216CrossRefPubMed

Deepak M, Sangli GK, Arun PC, Amit A (2005) Quantitative determination of the major saponin mixture bacoside A in Bacopa monnieri by HPLC. Phytochem Anal 16:24–29CrossRefPubMed

Desai ID (1984) Vitamin E analysis method for animal tissues. Methods Enzymol 105:138–143CrossRefPubMed

Di Maio M, De Maio E, Perrone F, Pignata S, Daniele B (2002) Hepatocellular carcinoma: systemic treatments. J Clin Gastroenterol 35(5):S109–S114CrossRefPubMed

Dodge JT, Mitchell C, Hanahan DJ (1963) The preparations and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys 100:119–130CrossRefPubMed

Drabkin DL, Austin JH (1932) Spectrophotometric studies. Part I. Spectrophotometric constants for common hemoglobin derivatives in human, dog, and rabbit blood. J Biol Chem 98:719–733

Elangovan V, Govindasamy S, Ramamoorthy N, Balasubramanian K (1995) In vitro studies on the anticancer activity of Bacopa monnieri. Fitoterapia 66:211–215

Esterbauer H, Schaur RJ, Zollner (1991) Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Rad Biol Med 11:81–128CrossRefPubMed

Frederiks WM, Noorden CJ, Aronson DC, Marx F, Bosch KS, Jonges GN, Vogels IM, James J (1990) Quantitative changes in acid phosphatases, alkaline phosphatases and 5′-nucleotidase activity in rat liver after experimentally induced cholestasis. Liver 10(3):158–166PubMedCrossRef

Gey KF (1993) Prospects for the prevention of free radical disease, regarding cancer and cardiovascular disease. Br Med Bull 49:679–699PubMed

Goel RK, Sairam K, Babu MD, Tavares IA, Raman A (2003) In vitro evaluation of Bacopa monniera on anti-helicobacter pylori activity and accumulation of prostaglandins. Phytomedicine 10:523–527CrossRefPubMed

Gultekin F, Delibas N, Sulhodtin Y, Kilinic I (2001) In vivo changes in antioxidant systems and protective role of melatonin and combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chloropyrifos ethyl in rats. Arch Toxicol 75:88–96CrossRefPubMed

Ha WS, Kim CK, Sung SH, Kang CB (2001) Study on the mechanism of multistep hepatotumorigenesis in rat: development of hepatotumorigenesis. J Vet Sci 2(1):53–58PubMed

Halliwell B, Gutteridge JMC (1989) Free radicals in biology and medicine, 2nd edn. Clarendon Press, Oxford, p 543

Hong WK, Sporn MB (1997) Recent advance in chemoprevention of cancer. Science 278:1073–1077CrossRefPubMed

Horwitt MK (1976) Vitamin E: a re-examination. Am J Clin Nutr 29:569–578PubMed

Hou CC, Lin SJ, Cheng JT, Hsu FL (2002) Bacopaside III, bacopasaponin G and bacopaside A, B and C from Bacopa monnieri. J Nat Prod 65:1759–1763CrossRefPubMed

IP C, Daw T (1978) Alterations in serum glycosyl transferase and 5′-nucleotidase in breast cancer patients. Cancer Res 38:723–728PubMed

Janani P, Sivakumari K, Parthasarathy C (2009) Hepatoprotective activity of bacoside A against N-nitrosodiethylamine-induced liver toxicity in adult rats. Cell Biol Toxicol 25:425–434CrossRefPubMed

King J (1965a) The dehydrogenases or oxidoreductases-lactate dehydrogense. In: Van D (ed) Practical clinical Enzymology. Nostrand Co. Ltd, London, pp 83–93

King J (1965b) In: Van D (eds) Practical clinical enzymology, Nostrand. Company, London, p 363

Kiritikar KR, Basu BD (1994) Indian medicinal plants, vol I. Bishen Singh Mahendrapal Singh, Dehradun, p 1816

Kono Y, Fridovich I (1982) Superoxide radicals inhibit catalase. J Biol Chem 257:5751–5754PubMed

Koss B, Greengard O (1982) Effect of neoplasms on the content and activity of alkaline phosphatase and gamma-glutamyl transpeptidase in uninvolved host tissues. Cancer Res 42(6):2146–2158PubMed

Kwe MC (1996) Tumors of the liver. In: Zakim D, Boyer TD (eds) Hepatology—a text book of liver disease. Saunders, Philadelphia, pp 1513–1548

Lockich JJ (1983) Determination of response I treatment of hepatic neoplasia. Semin Oncol 10:228–237

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275PubMed

Luczaj W, Skrzydlewska E (2003) DNA damage caused by lipid peroxidation products. Cell Mol Biol Letters 8:391–413

Macnab GM, Urbanowicz JM, Kew MC (1978) Carcinoembryonic antigen in hepatocellular cancer. Br J Cancer 38:51–54PubMed

Mccord JM, Fridovich I (1969) The utility of superoxide dismutase in studying free radical reactions. I. Radicals generated by the interaction of sulfite, dimethyl sulfoxide and oxygen. J Biol Chem 244:6056–6063PubMed

Meister A, Anderson ME (1983) Glutathione. Annu Rev Biochem 52:711–760CrossRefPubMed

Metlin C (1997) Chemoprevention: will it work? Int J Cancer 10:18–21CrossRef

Misra HP, Fridovich J (1972) The role of superoxide anion in the autooxidation of epinehrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175PubMed

Nadkarni KM (1976) Indian Materia Medica. Popular Prakashan Pvt Ltd, Bombay, pp 624–625

Nahon JL, Tratner I, Poliard A, Presse F, Poriet M, Gai A, Jose M, Trepat S (1988) Albumin and alpha feto protein gene expression in various nonhepatic rat tissues. J Biol Chem 263:11436–11442PubMed

Niedernhofer LJ, Daniels JS, Rouzer CA, Greene RE, Marnett LJ (2003) Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cells. J Biol Chem 278:31426–31433CrossRefPubMed

Niki E (1987) Interaction of ascorbate and α-tocopherol. Ann New York Acad Sci 498:186–199CrossRef

Oberley LW, Oberley TD (1986) Free radicals, cancer and aging. In: Johnson JE Jr, Walford R, Harman D, Miques (eds) Free radicals, Aging and degenerative diseases. Alan R Liss Inc, New York, pp 325–371

Ohkawa H, Ohnishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358CrossRefPubMed

Omaye ST, Turnbull JD, Sauberlich HE (1979) Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. Methods Enzymol 62:3–11CrossRefPubMed

Orlowski K, Meister A (1965) Isolation of gamma glutamyl transpeptidase from hog kidney. J Biol Chem 240:338–347PubMed

Pawar R, Gopalakrishnan C, Bhutani KK (2001) Dammarane triterpene saponin from Bacopa monniera as the superoxide inhibitor in polymorphonuclear cells. Planta Med 67:752–754CrossRefPubMed

Peers FG, Linsell CA (1973) Dietary aflatoxins and primary liver cancer: a population based study in Kenya. Br J Cancer 27:473–484PubMed

Piot HC, Sirica AE (1980) The stage of initiation and promotion in hepato-carcinogenesis. Biochem Biophys Acta 605:191–215

Pitot HC, Campbell HA, Maronpot R, Bawa N, Rizvi TA, Xu YH, Sargent L, Dragan Y, Pyron M (1989) Critical parameters in the quantitation of the stages of initiation, promotion and progression in one model of hepatocarcinogenesis in the rat. Toxicol Pathol 17(4 Pt 1):594–611PubMed

Plaa GL, Hewitt WR (1989) Detection and evaluation of chemically induced liver injury. In: Wallace Hayes A (ed) Principles and methods of toxicology. Raven Press, New York, pp 399–628

Prasad SB, Giri A (1999) Effect of cisplatin on the lactate dehydrogenase activity and its isozyme pattern in Dalton’s lymphoma bearing mice. Cytologia 64:259–267

Quaglia A, Bhattacharya S, Dhillon AP (2001) Limitations of the histopathological diagnosis and prognostic assessment of hepatocellular carcinoma. Histopathology 38:167–174CrossRefPubMed

Ray G, Batra S, Shkula NK, Deo S, Raina V, Asok S, Husain SA (2000) Lipid peroxidatio, free radicals production and antioxidant status in breast cancer. Breast Cancer Res Treat 59:163–170CrossRefPubMed

Rohini G, Sabitha KE, Devi CSS (2004) Bacopa monniera Linn. Extract modulates antioxidant and marker enzymes status in fibrosarcoma bearing rats. Indian J Exp Biol 42:776–780PubMed

Rosi F, Tabucehi A, Carhici F, Galient P, Laukia F, Zanoni L, Guerronti R, Marinello G, Pagoni R (1998) 5′-nucleotidase activity in lymphocytes from patients affected by B-cell chronic lymphocytic leukemia. Clin Biochem 31:269–272CrossRefPubMed

Rotruck JT, Pope AL, Ganther HE (1973) Selenium: biochemical role as a component of glutathione peroxidase, purification and assay. Science 179:588–590CrossRefPubMed

Russo A, Borrelli F (2005) Bacopa monniera, a reputed nootropic plant: an overview. Phytomedicine 12:305–317CrossRefPubMed

Russo A, Borrelli F, Campisi A, Acquaviva R, Raciti G, Vanella A (2003a) Nitric oxide related toxicity in cultured astrocytes: effect of Bacopa monniera. Life Sci 73:1517–1526CrossRefPubMed

Russo A, Izzo AA, Borrelli F, Renis M, Vanella A (2003b) Free radical scavenging capacity and protective effect on DNA damage of Bacopa monniera Linn. Phytother Res 17:870–875CrossRefPubMed

Sairam K, Rao CV, Babu MD, Goel RK (2001) Prophylactic and curative effects of Bacopa monniera in gastric ulcer models. Phytomedicine 8:423–430CrossRefPubMed

Savitha S, Tamilselvan J, Anusuyadevi M, Panneerselvam C (2005) Oxidative Stress on mitochondrial antioxidant defense system in the aging process: role of dl-alpha-lipoic acid and l-carnitine. Clin Chem Acta 355:173–180CrossRef

Schwartz MK, Bodansky O (1965) Serum 5′-nucleotidase in patients with cancer. Cancer 18:886–892CrossRefPubMed

Schwartz MA, West M, Walsch WS, Zimmerman HJ (1962) Serum enzymes in disease: glycolytic and oxidative enzymes and transaminases in patients with gastrointestinal carcinoma. Cancer 15:346CrossRef

Sell S, Becker FF (1978) Alpha feto protein. J Natl Cancer Inst 60:19–26PubMed

Sell S, Becker FF, Leffert H, Watable H (1976) Expression of an oncodevelopmental gene product (α-fetoprotein) during fetal development and adult oncogenesis. Cancer Res 36:4239–4249PubMed

Sen CK (1995) Oxygen toxicity and antioxidants: state of the art. Indian J Physiol Pharmacol 39:177–196PubMed

Simonsen R, Uirji MA (1984) Interpreting the profile of liver function tests in pediatric liver transplants. Clin Chem 30:1607–1610PubMed

Singh HK, Dhawn BN (1982) Neuropsychopharmacological effects of the ayurvedic nootropic Bacopa monnieri Linn. J Ethnopharmacol 5:205–214CrossRefPubMed

Singh HK, Rastogi RP, Srimal RC, Dhawan BN (1988) Effect of bacosides A and B on avoidance responses in rats. Phytother Res 2:70–75CrossRef

Sporn MB, Suh N (2002) Chemoprevention: an essential approach to controlling cancer. Nat Rev Cancer 2:537–543CrossRefPubMed

Staal GEJ, Visser J, Veger C (1969) Purification and properties of glutathione reductase of human erythrocytes. Biochim Biophys Acta 185:39–48PubMed

Stefanini M (1985) Enzymes, isozymes, and enzyme variants in the diagnosis of cancer. A short review. Cancer 55(9):1931–1936CrossRefPubMed

Sumathy T, Subramanian S, Govindasamy S, Balakrishna K, Veluchamy G (2001) Protective role of Bacopa monniera on morphine induced hepatotoxicity in rats. Phytother Res 15:643–645CrossRefPubMed

Tarao K, Takemiya S, Tamai S, Sugimasa Y, Ohkawa S, Akaike M, Tanabe H, Shimizu A, Yoshida M, Kakita A (1997) Relationship between the recurrence of hepatocellular carcinoma (HCC) and serum alanine aminotransferase levels in hepatectomized patients with hepatitis C virus associated cirrhosis and HCC. Cancer 79(4):688–694CrossRefPubMed

Tarao K, Rino Y, Ohkawa S, Shimizu A, Tamai S, Miyakawa K, Aoki H, Imada T, Shindo K, Okamato N, Totsuka S (1999) Association between high serum alanine aminotransferase levels and more rapid development and high rate incidence of hepatocellular carcinoma in patients with hepatitis C virus-associated cirrhosis. Cancer 86(4):589–595CrossRefPubMed

Thirunavukkarasu C, Prince Vijeya Singh J, Selvendiran K, Sakthisekaran D (2002) Chemopreventive efficacy of selenium against N-nitrosodiethylamine induced hepatoma in albino rats. Cell Biochem Funct 19:265–271CrossRef

Thomas P, Toth CA, Saini KS, Jessup JM, Steele GD (1990) The structure, metabolism and function of the carcinoembryonic antigen gene family. Biochim Biophys Acta 1032:177–189PubMed

Thompson JA, Grunert F, Zimmerman W (1991) Carcinoembryonic antigen gene family: molecular biology and clinical perspectives. J Clin Lab Anal 15:344–366CrossRef

Torel J, Cillard J, Cillard P (1986) Antioxidant activity of flavanoids and reactivity with peroxyl radical. Phytochemistry 25:383–385CrossRef

Toth CA, Thomas P, Broitman SA, Zamcheck N (1982) A new kupferr cell receptor mediating plasma clearance of carcinoembryonic antigen by the rat. Biochem J 15:377–381

Tripathi YB, Chaurasia S, Tripathi E, Upadhyay A, Dubey GP (1996) Bacopa monniera Linn. As an antioxidant: mechanism of action. Indian J Exp Biol 34:523–526PubMed

Uchida K, Sakai K, Itakura K, Osawa T, Toyokuni S (1997) Protein modification by lipid peroxidation products: formation of malondialdehyde-derived Nε-(2-propenal)lysine in proteins. Arch Biochem Biophys 346:45–52CrossRefPubMed

Verna L, Whysner J, Williams GM (1996) N-nitrosodiethylamine mechanistic data and risk assessment: bioactivation, DNA-adduct formation, mutagenicity, and tumor initiation. Pharmacol Ther 71:57–81CrossRefPubMed

Yoshiki Y, Kim JH, Okubo K, Nagoya I, Sakabe T, Tamura N (1995) A saponin conjugated with, 3-dihydro-2, 5-dihydroxy-6-methyl-4H-pyran-4-one from dolichos lablab. Phytochemistry 38:229–231CrossRefPubMed

Yoshiki K, Kudou S, Okubo K (1998) Relationship between chemical structures and biological activities of triterpinoid saponins from soyabean. Biosci Biotech Biochem 62:2291–2299CrossRef

Yu BP (1994) Cellular defense against damage from reactive oxygen species. Physiol Rev 74:136–162

Yu AS, Keeffe EB (2003) Management of hepatocellular carcinoma. Rev Gastroenterol Disord 3(1):8–24PubMed

Zamcheck N, Steele GD, Thomas P, Meyer RJ (1986) Use of carcinoembryonic antigen in the monitoring of patients. In: Rose NR, Friedman H, Fahey JL (eds) The manual of clinical immunology. American Society for Microbiology, Washington, DC, pp 802–809

Titel: Chemopreventive effect of bacoside A on N-nitrosodiethylamine-induced hepatocarcinogenesis in rats
verfasst von: Panneerselvam Janani
Kanakarajan Sivakumari
Arumugam Geetha
Baskaran Ravisankar
Chandrakesan Parthasarathy
Publikationsdatum: 01.05.2010
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 5/2010
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-009-0715-0

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Chemopreventive effect of bacoside A on N-nitrosodiethylamine-induced hepatocarcinogenesis in rats