Erschienen in:
01.12.2015 | Research Article
Parameterization of hyperpolarized 13C-bicarbonate-dissolution dynamic nuclear polarization
verfasst von:
David Johannes Scholz, Angela M. Otto, Josef Hintermair, Franz Schilling, Annette Frank, Ulrich Köllisch, Martin A. Janich, Rolf F. Schulte, Markus Schwaiger, Axel Haase, Marion I. Menzel
Erschienen in:
Magnetic Resonance Materials in Physics, Biology and Medicine
|
Ausgabe 6/2015
Einloggen, um Zugang zu erhalten
Abstract
Objective
13C metabolic MRI using hyperpolarized 13C-bicarbonate enables preclinical detection of pH. To improve signal-to-noise ratio, experimental procedures were refined, and the influence of pH, buffer capacity, temperature, and field strength were investigated.
Materials and methods
Bicarbonate preparation was investigated. Bicarbonate was prepared and applied in spectroscopy at 1, 3, 14 T using pure dissolution, culture medium, and MCF-7 cell spheroids. Healthy rats were imaged by spectral–spatial spiral acquisition for spatial and temporal bicarbonate distribution, pH mapping, and signal decay analysis.
Results
An optimized preparation technique for maximum solubility of 6 mol/L and polarization levels of 19–21 % is presented; T1 and SNR dependency on field strength, buffer capacity, and pH was investigated. pH mapping in vivo is demonstrated.
Conclusion
An optimized bicarbonate preparation and experimental procedure provided improved T1 and SNR values, allowing in vitro and in vivo applications.