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Journal of Assisted Reproduction and Genetics

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01.10.2012 | ASSISTED REPRODUCTION TECHNOLOGIES

Follicular fluid protein content (FSH, LH, PG4, E2 and AMH) and polar body aneuploidy

verfasst von: I. Hammoud, F. Vialard, M. Bergere, M. Albert, D. Molina Gomes, M. Adler, L. Malagrida, M. Bailly, R. Wainer, J. Selva

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 10/2012

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Abstract

Purpose

Our objective was to identify a marker for oocyte aneuploidy in follicular fluid (FF) in women with an increased risk of oocyte aneuploidy after controlled ovarian hyperstimulation.

Materials and methods

Three groups of oocytes were constituted for polar body screening by FISH (chromosomes 13, 16, 18, 21 and 22): Group 1, advanced maternal age (n = 156); Group 2, implantation failure (i.e. no pregnancy after the transfer of more than 10 embryos; n = 101) and Group 3, implantation failure and advanced maternal age (n = 56). FSH and other proteins were assayed in the corresponding FF samples.

Results

Of the 313 oocytes assessed, 35.78 % were abnormal. We found a significant difference between the follicular FSH levels in normal oocytes and abnormal oocytes (4.85 ± 1.75 IU/L vs. 5.41 ± 2.47 IU/L, respectively; p = 0.021). We found that the greater the number of chromosomal abnormalities per oocyte (between 0 and 3), the higher the follicular FSH level.

Conclusion

High FF FSH levels were associated with oocyte aneuploidy in women having undergone controlled ovarian hyperstimulation.

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Titel: Follicular fluid protein content (FSH, LH, PG4, E2 and AMH) and polar body aneuploidy
verfasst von: I. Hammoud
F. Vialard
M. Bergere
M. Albert
D. Molina Gomes
M. Adler
L. Malagrida
M. Bailly
R. Wainer
J. Selva
Publikationsdatum: 01.10.2012
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 10/2012
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-012-9841-8

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Follicular fluid protein content (FSH, LH, PG4, E2 and AMH) and polar body aneuploidy