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Journal of Assisted Reproduction and Genetics

Erschienen in:

05.02.2018 | Reproductive Physiology and Disease

In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration

verfasst von: Lusine Aghajanova, Sahar Houshdaran, Shaina Balayan, Evelina Manvelyan, Juan C. Irwin, Heather G. Huddleston, Linda C. Giudice

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 5/2018

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Abstract

Purpose

The study aims to test the hypothesis that platelet-rich plasma (PRP) stimulates cellular processes involved in endometrial regeneration relevant to clinical management of poor endometrial growth or intrauterine scarring.

Methods

Human endometrial stromal fibroblasts (eSF), endometrial mesenchymal stem cells (eMSC), bone marrow-derived mesenchymal stem cells (BM-MSC), and Ishikawa endometrial adenocarcinoma cells (IC) were cultured with/without 5% activated (a) PRP, non-activated (na) PRP, aPPP (platelet-poor-plasma), and naPPP. Treatment effects were evaluated with cell proliferation (WST-1), wound healing, and chemotaxis Transwell migration assays. Mesenchymal-to-epithelial transition (MET) was evaluated by cytokeratin and vimentin expression. Differential gene expression of various markers was analyzed by multiplex Q-PCR.

Results

Activated PRP enhanced migration of all cell types, compared to naPRP, aPPP, naPPP, and vehicle controls, in a time-dependent manner (p < 0.05). The WST-1 assay showed increased stromal and mesenchymal cell proliferation by aPRP vs. naPRP, aPPP, and naPPP (p < 0.05), while IC proliferation was enhanced by aPRP and aPPP (p < 0.05). There was no evidence of MET. Expressions of MMP1, MMP3, MMP7, and MMP26 were increased by aPRP (p < 0.05) in eMSC and eSF. Transcripts for inflammation markers/chemokines were upregulated by aPRP vs. aPPP (p < 0.05) in eMSC and eSF. No difference in estrogen or progesterone receptor mRNAs was observed.

Conclusions

This is the first study evaluating the effect of PRP on different human endometrial cells involved in tissue regeneration. These data provide an initial ex vivo proof of principle for autologous PRP to promote endometrial regeneration in clinical situations with compromised endometrial growth and scarring.

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Titel: In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration
verfasst von: Lusine Aghajanova
Sahar Houshdaran
Shaina Balayan
Evelina Manvelyan
Juan C. Irwin
Heather G. Huddleston
Linda C. Giudice
Publikationsdatum: 05.02.2018
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 5/2018
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-018-1130-8

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In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration