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01.01.2013 | Original Paper

Vasorelaxant activity of indole alkaloids from Tabernaemontana dichotoma

verfasst von: Kazumasa Zaima, Ikumi Koga, Nobuhide Iwasawa, Takahiro Hosoya, Yusuke Hirasawa, Toshio Kaneda, Intan Safinar Ismail, Nordin Hj Lajis, Hiroshi Morita

Erschienen in: Journal of Natural Medicines | Ausgabe 1/2013

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Abstract

The aim of this study was to search for bioactive natural products from medicinal plants targeting vasorelaxant activity and we found the methanol extract from bark of Tabernaemontana dichotoma showed vasorelaxant activity on rat aorta. We isolated eight indole alkaloids including 10-methoxyalstonerine (1), a new macroline type indole alkaloid, from bark of T. dichotoma. These were respectively identified as 10-methoxyaffinisine (2), lochnerine (3), cathafoline (4), (−)-alstonerine (5), 19,20-dehydro-10-methoxytalcarpine (6), alstonisine (7), and alstonal (8) based on spectroscopic analysis. Among them, sarpagine type (2 and 3), akuammiline type (4), and macroline oxindole type (7 and 8) showed potent vasorelaxant activity. Mechanism of action on vasorelaxant activity of 10-methoxyaffinisine (2), cathafoline (4), and alstonisine (7) was clarified. Effects of 10-methoxyaffinisine (2), cathafoline (4), and alstonisine (7) were partially mediated the NO release from endothelial cells. Furthermore, 10-methoxyaffinisine (2) and alstonisine (7) attribute to the inhibitory effect of VDC and ROC, and cathafoline (4) have inhibitory effect on Ca²⁺ influx via ROC. In addition, 10-methoxyaffinisine (2) as a major compound from bark of T. dichotoma showed hypotensive effect on normotensive rats in vivo.

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Titel: Vasorelaxant activity of indole alkaloids from Tabernaemontana dichotoma
verfasst von: Kazumasa Zaima
Ikumi Koga
Nobuhide Iwasawa
Takahiro Hosoya
Yusuke Hirasawa
Toshio Kaneda
Intan Safinar Ismail
Nordin Hj Lajis
Hiroshi Morita
Publikationsdatum: 01.01.2013
Verlag: Springer Japan
Erschienen in: Journal of Natural Medicines / Ausgabe 1/2013
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-012-0638-y

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Vasorelaxant activity of indole alkaloids from Tabernaemontana dichotoma

Abstract

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Abstract

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